Effect of FGF-2 and sciatic nerve grafting on ChAT expression in dorsal root ganglia neurons of spinal cord transected rats

Guzen, Fausto Pierdoná; Araújo, Dayane Pessoa de; Lucena, Eudes Euler de Souza; Morais, Hécio Henrique Araújo de; Cavalcanti, José Rodolfo Lopes de Paiva; Nascimento Junior, Expedito Silva do; Costa, Miriam Stela Maris de Oliveira; Cavalcante, Jeferson de Souza

Effect of FGF-2 and sciatic nerve grafting on ChAT expression in dorsal root ganglia neurons of spinal cord transected rats

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dc.contributor.author	Guzen, Fausto Pierdoná
dc.contributor.author	Araújo, Dayane Pessoa de
dc.contributor.author	Lucena, Eudes Euler de Souza
dc.contributor.author	Morais, Hécio Henrique Araújo de
dc.contributor.author	Cavalcanti, José Rodolfo Lopes de Paiva
dc.contributor.author	Nascimento Junior, Expedito Silva do
dc.contributor.author	Costa, Miriam Stela Maris de Oliveira
dc.contributor.author	Cavalcante, Jeferson de Souza
dc.date.accessioned	2021-03-05T11:06:24Z
dc.date.available	2021-03-05T11:06:24Z
dc.date.issued	2016-03
dc.description.resumo	Neurotrophic factors and peripheral nerves are known to be good substrates for bridging CNS trauma. The involvement of fibroblast growth factor-2 (FGF-2) activation in the dorsal root ganglion (DRG) was examined following spinal cord injury in the rat. We evaluated whether FGF-2 increases the ability of a sciatic nerve graft to enhance neuronal plasticity, in a gap promoted by complete transection of the spinal cord. The rats were subjected to a 4 mm-long gap at low thoracic level and were repaired with saline (Saline or control group, n = 10), or fragment of the sciatic nerve (Nerve group, n = 10), or fragment of the sciatic nerve to which FGF-2 (Nerve + FGF-2 group, n = 10) had been added immediately after lesion. The effects of the FGF-2 and fragment of the sciatic nerve grafts on neuronal plasticity were investigated using choline acetyl transferase (ChAT)-immunoreactivity of neurons in the dorsal root ganglion after 8 weeks. Preservation of the area and diameter of neuronal cell bodies in dorsal root ganglion (DRG) was seen in animals treated with the sciatic nerve, an effect enhanced by the addition of FGF-2. Thus, the addition of exogenous FGF-2 to a sciatic nerve fragment grafted in a gap of the rat spinal cord submitted to complete transection was able to improve neuroprotection in the DRG. The results emphasized that the manipulation of the microenvironment in the wound might amplify the regenerative capacity of peripheral neurons	pt_BR
dc.identifier.citation	GUZEN, Fausto Pierdoná; ARAÚJO, Dayane Pessoa de; LUCENA, Eudes Euler de Souza; MORAIS, Hécio Henrique Araújo de; CAVALCANTI, José Rodolfo Lopes de Paiva; NASCIMENTO JUNIOR, Expedito Silva do; COSTA, Miriam Stela Maris de Oliveira; CAVALCANTE, Jeferson de Souza. Effect of FGF-2 and sciatic nerve grafting on ChAT expression in dorsal root ganglia neurons of spinal cord transected rats. Neuroscience Letters, [s. l.], v. 616, p. 43-48, mar. 2016. Elsevier BV. Disponível em: https://www.sciencedirect.com/science/article/abs/pii/S0304394015301130?via%3Dihub#!. Acesso em: 23 jul. 2020. http://dx.doi.org/10.1016/j.neulet.2015.08.043	pt_BR
dc.identifier.doi	10.1016/j.neulet.2015.08.043
dc.identifier.issn	0304-3940 (print)
dc.identifier.uri	https://repositorio.ufrn.br/handle/123456789/31695
dc.language	en	pt_BR
dc.publisher	Elsevier	pt_BR
dc.rights	Attribution 3.0 Brazil	*
dc.rights.uri	http://creativecommons.org/licenses/by/3.0/br/	*
dc.subject	Dorsal root ganglia	pt_BR
dc.subject	Fibroblastic growth factor-2	pt_BR
dc.subject	Neuroprotection	pt_BR
dc.subject	Sciatic nerve graft	pt_BR
dc.subject	Spinal cord	pt_BR
dc.title	Effect of FGF-2 and sciatic nerve grafting on ChAT expression in dorsal root ganglia neurons of spinal cord transected rats	pt_BR
dc.type	article	pt_BR