CCS - DFAR - Artigos publicados em periódicos

URI Permanente para esta coleçãohttps://repositorio.ufrn.br/handle/1/46

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Chitooligosaccharides enzymatic production by Metarhizium anisopliae
(Springer Science and Business Media LLC, 2010-02-18) Santos, Everaldo Silvino dos; Assis, Cristiane Fernandes de; Araújo, Nathália Kelly; Pagnoncelli, Maria Giovana Binder; Pedrini, Márcia Regina da Silva; Macedo, Gorete Ribeiro de
The products of chitosan hydrolysis are chitooligosaccharides and are used mainly for medical applications due to their specific biological activities. The objective of this study was to detect and identify the products of enzymatic hydrolysis of chitosan (dimers to hexamers) using a crude extract of chitosanolytic enzymes produced by the fungus Metarhizium anisopliae. These fungus was able to produce, during 48 h cultivation in a medium containing chitosan, chitooligosaccharides ranging from dimers, trimers, tetramers and pentamers at concentrations 0.2, 0.19, 0.06, 0.04 mg/mL, respectively, and the enzymatic activity was 2.5 U/L. Using the crude enzyme extract for chitosan hydrolysis, we detected the presence of dimers to hexamers at hydrolysis times of 10, 20, 30, 40, 50 and 60 min of enzymatic reaction, but the yields were higher at 10 min (54%). The hexamers was obtained only with 30 min of reaction with concentration of 0.004 mg/mL
Gelatin nanoparticles enable water dispersibility and potentialize the antimicrobial activity of Buriti (Mauritia flexuosa) oil
(BMC Biotechnology, 2020-10-16) Passos, Thais Souza; Castro, Gabrielle Mahara Martins Azevedo; Nascimento, Sara Sayonara da Cruz; Medeiros, Isaiane; Araújo, Nathália Kelly; Maciel, Bruna Leal Lima; Padilha, Carlos Eduardo; Ramalho, Adriana Margarida Zanbotto; Sousa Júnior, Francisco Canidé; Assis, Cristiane Fernandes de; https://orcid.org/0000-0003-2054-1544; https://orcid.org/0000-0003-4541-3952; https://orcid.org/0000-0001-6429-1538; https://orcid.org/0000-0003-2042-4348; https://orcid.org/0000-0001-7595-5395
Background: Buriti oil presents numerous health benefits, but due to its lipophilic nature and high oxidation, it is impossible to incorporate it into aqueous food matrices. Thus, the present study evaluated whether powder nanoparticles based on porcine gelatin (OPG) and in combination with sodium alginate (OAG) containing buriti oil obtained by O/W emulsification followed by freeze-drying enabled water dispersibility and preserved or increased the antimicrobial activity of the oil. Results: OPG presented spherical shape, smooth surface, smaller particle size and polydispersity index [51.0 (6.07) nm and 0.40 (0.05)], and better chemical interaction between the nonpolar amino acids and the hydrophobic oil chain. OPG also presented a higher dispersibility percentage [85.62% (7.82)] than OAG [50.19% (7.24)] (p < 0.05), and significantly increased the antimicrobial activity of the oil by 59, 62, and 43% for Pseudomonas aeruginosa, Klebsiellapneumonia, and Staphylococcus aureus, respectively. Conclusions: Thus, nanoencapsulation in gelatin is a promising strategy to increase the potential to use buriti oil in foods

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