CB - DFIS - Artigos publicados em periódicos

URI Permanente para esta coleçãohttps://repositorio.ufrn.br/handle/1/16

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Development of new techniques for behavioral evaluation In animals submitted to facial nerve damage
(Research Journal of Pharmaceutical, Biological and Chemical Sciences, 2019) Oliveira, Lucidio Clebeson de; Oliveira, Eligleidson José Vidal de; Lucena, Eudes Euler de Souza; Cavalcanti, José Rodolfo Lopes de Paiva; Fernandes, João Paulo Costa; Souza Júnior, José Edvan de; Cavalcante, Jeferson de Souza; Azevedo, Eduardo Pereira de; Rêgo, Amália Cinthia Meneses do; Araújo Filho, Irami; Castro, Aline Brito Ferreira de; Guzen, Fausto Pierdoná
Three new methods for evaluating facial nerve injury were developed: Spontaneous ocular opening, vibrissae reflex and resistance to upper eyelid lifting. Nerve crush injury was performed by pressing the mice’s facial nerve with forceps for 30 seconds. Scores were given based on the comparison between the behavioral assessment of the injured and non-injured sides. Facial nerve injury is currently evaluated by observing the movement of the mice’s vibrissae and the ocular closure. In this study, three additional tests are proposed. Therefore, a wider evaluation can be accomplished where all the aspects of the facial mimicry can be assessed. The use of the 3 new methods described in this study, in addition to the 2 methods currently used, allows a complete behavioral assessment of facial nerve lesions as several behavioral aspects related to these injuries can be evaluated
Expansion and phenotypic changes of mouse bone marrow mesenchymal cells cultured with FGF-2 and facial nerve-conditioned medium
(Sociedade Chilena de Anatomía, 2018-09) Lucena, Eudes Euler de Souza; Morais, Hécio Henrique Araújo de; Araújo, Dayane Pessoa de; Cavalcanti, José Rodolfo Lopes de Paiva; Azevedo, Eduardo Pereira de; Queiroz, Dinalva Brito de; Botelho, Marco Antônio; Rêgo, Amália Cinthia Meneses do; Araújo Filho, Irami; Barboza, Carlos Augusto Galvão; Nascimento Júnior, Expedito Silva do; Costa, Miriam Stela Maris de Oliveira; Cavalcante, Jeferson de Sousa; Guzen, Fausto Pierdoná
Mesenchymal cells (MCs) exhibit great regenerative potential due to their intrinsic properties and ability to restore tissue function, either directly through transdifferentiation or indirectly through paracrine effects. This study aimed to evaluate morphometric and phenotypic changes in MCs grown with facial nerve-conditioned medium in the presence or absence of fibroblast growth factor 2 (FGF-2). For quantitative phenotypic analysis, the expression of GFAP, OX-42, MAP-2, β-tubulin III, NeuN, and NF-200 was analyzed by immunocytochemistry. Cells cultured with facial nerve-conditioned medium in the presence of FGF-2 expressed GFAP, OX-42, MAP-2, β-tubulin III, NeuN, and NF-200. On average, the area and perimeter of GFAP-positive cells were higher in the group cultured with facial nerve-conditioned medium compared to the group cultured with conditioned medium and FGF-2 (p=0.0001). This study demonstrated the plasticity of MCs for neuronal and glial lineages and opens up new research perspectives in cell therapy and trans.differentiation

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