Activity toward bruchid pest of a kunitz-type inhibitor from seeds of the algaroba tree (prosopis juliflora D.C.)

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dc.contributor.authorMorais, Ana Heloneida de Araújo
dc.contributor.authorOliveira, Adeliana
dc.contributor.authorPereira, Railene
dc.contributor.authorLima, Liziane
dc.contributor.authorFranco, Octávio Luiz
dc.contributor.authorBloch Júnior, Carlos
dc.contributor.authorSá, Maria Fátima Grossi de
dc.contributor.authorSales, Maurício
dc.date.accessioned2024-04-12T22:33:40Z
dc.date.available2024-04-12T22:33:40Z
dc.date.issued2002
dc.description.resumoA proteinaceous inhibitor with high activity against papain was found in seeds of the xerophytic algaroba tree (Prosopis julifora). The proteinase inhibitor Pj was purified using Sephacryl S-200 gel filtration followed by reverse-phase high-performance liquid chromatography on a Vidac 18 TP. Inhibitor Pj showed a Mr of 20,000 on sodium dodecyl sulfate–polyacrylamide gel electrophoresis and a Mr of 19.2K by mass spectrometry. The inhibition of papain by the Pj inhibitor was the noncompetitive type, with a Ki value of 0.59 3 1029 M. The gelatinase activity of papain was strongly inhibited by Pj too. The N-terminal amino acid sequence of the Pj inhibitor showed homology with the N-terminal amino acid sequence of the Kunitzproteinase inhibitor family. Pj was strongly effective against digestive proteinases from bean weevil Acanthoscelides obtectus and cowpea weevil Callosobruchus maculatus and was moderately active toward midgut proteinases from pod weevil Mimosestes mimosae and Mexican bean weevil Zabrotes subfasciatus. The data shown here suggest that the protein present in algaroba seeds is involved with defense responses to insects and may be an important tool to be used in engineering plants resistant to bean weevilpt_BR
dc.identifier.citationOLIVEIRA, Adeliana; PEREIRA, Railene; LIMA, Liziane; MORAIS, Ana Heloneida Araújo; FRANCO, Octávio Luiz; BLOCH JÚNIOR, Carlos; SÁ, Maria Fátima Grossi de; SALES, Maurício. Activity toward bruchid pest of a kunitz-type inhibitor from seeds of the algaroba tree (prosopis juliflora D.C.). Pesticide Biochemistry and Physiology, [S.l.], , v. 72, p. 122-132, 2002. Disponível em: https://www.sciencedirect.com/science/article/pii/S004835750192591X. Acesso em: 12 abr. 2024.pt_BR
dc.identifier.doihttps://doi.org/10.1006/pest.2001.2591
dc.identifier.urihttps://repositorio.ufrn.br/handle/123456789/58146
dc.languageenpt_BR
dc.publisherPesticide Biochemistry and Physiologypt_BR
dc.rightsAttribution 3.0 Brazil*
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/br/*
dc.subjectProsopis juliflorapt_BR
dc.subjectPlant defensept_BR
dc.subjectBean weevilspt_BR
dc.subjectPhytocystatinpt_BR
dc.subjectBruchidspt_BR
dc.titleActivity toward bruchid pest of a kunitz-type inhibitor from seeds of the algaroba tree (prosopis juliflora D.C.)pt_BR
dc.typearticlept_BR

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