Nanopartículas de poli-hidroxibutirato-co-valerato como suporte para a imobilização da lipase de Candida antarctica fração B

Fernandes, Ilizandra A.; Nyari, Nádia L. D.; Oliveira, José Vladimir de; Rigo, Elisandra; Souza, Maria Cristiane M. de; Gonçalves, Luciana R. B.; Pergher, Sibele Berenice Castellã; Oliveira, Débora de

Nanopartículas de poli-hidroxibutirato-co-valerato como suporte para a imobilização da lipase de Candida antarctica fração B

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dc.contributor.author	Fernandes, Ilizandra A.
dc.contributor.author	Nyari, Nádia L. D.
dc.contributor.author	Oliveira, José Vladimir de
dc.contributor.author	Rigo, Elisandra
dc.contributor.author	Souza, Maria Cristiane M. de
dc.contributor.author	Gonçalves, Luciana R. B.
dc.contributor.author	Pergher, Sibele Berenice Castellã
dc.contributor.author	Oliveira, Débora de
dc.date.accessioned	2020-07-15T01:00:52Z
dc.date.available	2020-07-15T01:00:52Z
dc.date.issued	2014-04
dc.description.resumo	This work evaluates the immobilization of Candida antarctica lipase (Fraction B) using poly(hydroxybutyrate-co-hydroxyvalerate) (PHBV) nanoparticles as support. The effects of immobilization time (30-150 min) and pH (5-10) on lipase loading were evaluated. The stability of the immobilized enzyme towards temperature (40, 60, and 80 ºC), reuse and storage (at 4 ºC) were also determined. Furthermore, to assess its potential application in a system of interest, the immobilized lipase was used as a catalyst in the esterification of geraniol with oleic acid. The results indicated a time of 120 minutes and pH of 7 as optimal for immobilization. A 21 hour exposure of the PHBV-lipase derivative to 60 ºC showed a 33% reduction of the initial activity while storage at 4 ºC led to a residual activity (5% of the original activity). The derivative was used without significant loss of activity for 4 successive cycles. The use of the immobilized lipase as a catalyst in the production of geranyl oleate led to about 88% conversion of the initial reactants to products	pt_BR
dc.identifier.citation	FERNANDES, I. A.; NYARI, N. L. D.; OLIVEIRA, J. V. de; RIGO, E.; SOUZA, M. C. M. de; GONÇALVES, L. R. B.; PERGHER, Sibele Berenice Castellã; OLIVEIRA, D. de..Nanopartículas de poli-hidroxibutirato-co-valerato como suporte para a imobilização da lipase de Candida antarctica fração B. Química Nova (Impresso), v. 37, n. 2, p. 331-336, abr. 2014. ISSN 0100-4042. Disponível em: http://static.sites.sbq.org.br/quimicanova.sbq.org.br/pdf/v37n2a22.pdf. Acesso em 25 maio 2020. http://dx.doi.org/10.5935/0100-4042.20140055	pt_BR
dc.identifier.doi	10.5935/0100-4042.20140055
dc.identifier.issn	0100-4042
dc.identifier.uri	https://repositorio.ufrn.br/jspui/handle/123456789/29631
dc.language	pt_BR	pt_BR
dc.publisher	Sociedade Brasileira de Química	pt_BR
dc.rights	Attribution-NonCommercial 3.0 Brazil	*
dc.rights.uri	http://creativecommons.org/licenses/by-nc/3.0/br/	*
dc.subject	Nanoparticles	pt_BR
dc.subject	Immobilization	pt_BR
dc.subject	Lipases	pt_BR
dc.title	Nanopartículas de poli-hidroxibutirato-co-valerato como suporte para a imobilização da lipase de Candida antarctica fração B	pt_BR
dc.title.alternative	Nanoparticles of poly(hydroxybutyrate-co-hydroxyvalerate) as support for the immobilization of Candida antarctica lipase (fraction B)	pt_BR
dc.type	article	pt_BR