Assessment of two immobilized lipases activity and stability to low temperatures in organic solvents under ultrasound-assisted irradiation

Batistella, Luciane; Ustra, Mara K.; Richetti, Aline; Pergher, Sibele Berenice Castellã; Treichel, Helen; Oliveira, J. V.; Lerin, Lindomar; Oliveira, Débora de

Assessment of two immobilized lipases activity and stability to low temperatures in organic solvents under ultrasound-assisted irradiation

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dc.contributor.author	Batistella, Luciane
dc.contributor.author	Ustra, Mara K.
dc.contributor.author	Richetti, Aline
dc.contributor.author	Pergher, Sibele Berenice Castellã
dc.contributor.author	Treichel, Helen
dc.contributor.author	Oliveira, J. V.
dc.contributor.author	Lerin, Lindomar
dc.contributor.author	Oliveira, Débora de
dc.date.accessioned	2020-07-01T09:12:25Z
dc.date.available	2020-07-01T09:12:25Z
dc.date.issued	2012-03
dc.description.resumo	Both stability and catalytic activity of two commercial immobilized lipases were investigated in the presence of different organic solvents in ultrasound-assisted system. In a general way, for Novozym 435, the use of ethanol as solvent led to a loss of activity of 35% after 10 h of contact. The use of iso-octane conducted to a gradual increase in lipase activity in relation to the contact time, reaching a maximum value of relative activity of 126%. For Lipozyme RM IM, after 5 h of exposure, the enzyme presented no residual activity when ethanol was used as solvent. The solvents tert-butanol and iso-octane showed an enhancement of about 20 and 17% in the enzyme activity in 6 h of exposure, respectively. Novozym 435 and Lipozyme IM presented high stability to storage after treatment under ultrasound-assisted system using n-hexane and tert-butanol as solvents.	pt_BR
dc.identifier.citation	BATISTELLA, Luciane; USTRA, Mara K.; RICHETTI, Aline; PERGHER, Sibele Berenice Castellã; TREICHEL, Helen; OLIVEIRA, J. V.; LERIN, Lindomar; OLIVEIRA, Débora de. Assessment of two immobilized lipases activity and stability to low temperatures in organic solvents under ultrasound-assisted irradiation. Bioprocess And Biosystems Engineering, [s.l.], v. 35, n. 3, p. 351-358, mar. 2012. Disponível em: https://link.springer.com/article/10.1007/s00449-011-0573-4. Acesso em: 19 maio 2020. https://doi.org/10.1007/s00449-011-0573-4.	pt_BR
dc.identifier.doi	10.1007/s00449-011-0573-4
dc.identifier.issn	1615-7591 (print)
dc.identifier.uri	https://repositorio.ufrn.br/jspui/handle/123456789/29393
dc.language	en	pt_BR
dc.publisher	Springer	pt_BR
dc.rights	Attribution 3.0 Brazil	*
dc.rights.uri	http://creativecommons.org/licenses/by/3.0/br/	*
dc.subject	Lipases	pt_BR
dc.subject	Enzyme activity	pt_BR
dc.subject	Ultrasound system	pt_BR
dc.subject	Enzyme stability	pt_BR
dc.title	Assessment of two immobilized lipases activity and stability to low temperatures in organic solvents under ultrasound-assisted irradiation	pt_BR
dc.type	article	pt_BR