Design of a lipase-nano particle biocatalysts and its use in the kinetic resolution of medicament precursors

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dc.contributor.authorBezerra, Rayanne Mendes
dc.contributor.authorAndrade Neto, Davino M.
dc.contributor.authorGalvão, Wesley S.
dc.contributor.authorRios, Nathalia Saraiva
dc.contributor.authorCarvalho, Ana Caroline Lustosa de Melo
dc.contributor.authorCorrêa, Marcio Assolin
dc.contributor.authorBohn, Felipe
dc.contributor.authorFernandez-Lafuente, Roberto
dc.contributor.authorFechine, Pierre B.A.
dc.contributor.authorMattos, Marcos Carlos de
dc.contributor.authorSantos, José Cleiton Sousa dos
dc.contributor.authorGonçalves, Luciana R.B.
dc.date.accessioned2020-06-16T18:55:46Z
dc.date.available2020-06-16T18:55:46Z
dc.date.issued2017-09-15
dc.description.resumoSuperparamagnetic iron oxide nanoparticles (Fe3O4) were prepared by the co-precipitation method and functionalized with 3-amino-propyltriethoxysilane (APTES) or branched-polyethylenimine (PEI). After that, two parallels methods to immobilize the lipase from Thermomyces lanuginosus (TLL) were performed: the first one by ionic exchange and the second one by covalent attachment after the functionalization of the support with glutaraldehyde (GA). X-ray powder diffraction, magnetometry and infrared spectroscopy analysis were used to characterize the TLL preparations. These analyses showed that all samples presented superparamagnetic properties even after the immobilization procedure. The SPMN (superparamagnetic nanoparticle) @APTES covalent preparation had around 450 min of half-life time at pH 7.0 and 70 °C while that of the free enzyme was 46 min. These biocatalysts were evaluated in the kinetic resolution of rac-1-methyl-2-(2,6-dimethylphenoxy)ethyl acetate in different co-solvents (acetonitrile, isopropanol, ethyl ether and tetrahydrofuran). The best results were for the enzyme/substrate ratio of 2:1, in the presence of the ethyl ether or THF (20% v/v both) at 30 °C during 24 h with the SPMN@PEI-TLL biocatalyst. The conversion attained was 50% and the enantiomeric excess of the product was 99%. The new SPMN support are an excellent strategy to easy recovery of the biocatalyst by applying a magnetic fieldpt_BR
dc.identifier.citationBEZERRA, Rayanne M.; ANDRADE NETO, Davino M.; GALVÃO, Wesley S.; RIOS, Nathalia S.; CARVALHO, Ana Caroline L. de M.; CORREA, Marcio A.; BOHN, Felipe; FERNANDEZ-LAFUENTE, Roberto; FECHINE, Pierre B.a.; MATTOS, Marcos C. de. Design of a lipase-nano particle biocatalysts and its use in the kinetic resolution of medicament precursors. Biochemical Engineering Journal, [s.l.], v. 125, p. 104-115, set. 2017. Elsevier BV. Disponível em: https://www.sciencedirect.com/science/article/pii/S1369703X17301493?via%3Dihub. Acesso em: 10 jun. 2020. http://dx.doi.org/10.1016/j.bej.2017.05.024.pt_BR
dc.identifier.doi10.1016/j.bej.2017.05.024
dc.identifier.issn1369-703X
dc.identifier.urihttps://repositorio.ufrn.br/jspui/handle/123456789/29275
dc.languageenpt_BR
dc.publisherElsevierpt_BR
dc.subjectBiocatalystpt_BR
dc.subjectSuperparamagnetic nanoparticlespt_BR
dc.subject3-Amino-propyltriethoxysilanept_BR
dc.subjectBranched-polyethyleniminept_BR
dc.subjectKinetic resolutionpt_BR
dc.titleDesign of a lipase-nano particle biocatalysts and its use in the kinetic resolution of medicament precursorspt_BR
dc.typearticlept_BR

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