Chronic in vivo optogenetic stimulation modulates neuronal excitability, spine morphology, and Hebbian plasticity in the mouse hippocampus

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dc.contributor.authorMoulin, Thiago C.
dc.contributor.authorPetiz, Lyvia L.
dc.contributor.authorRayêe, Danielle
dc.contributor.authorWinne, Jessica
dc.contributor.authorMaia, Roberto G.
dc.contributor.authorCruz, Rafael V. Lima da
dc.contributor.authorAmaral, Olavo B.
dc.contributor.authorLeão, Richardson Naves
dc.date.accessioned2019-03-18T16:33:09Z
dc.date.available2019-03-18T16:33:09Z
dc.date.issued2019-02-15
dc.description.resumoProlonged increases in excitation can trigger cell‐wide homeostatic responses in neurons, altering membrane channels, promoting morphological changes, and ultimately reducing synaptic weights. However, how synaptic downscaling interacts with classical forms of Hebbian plasticity is still unclear. In this study, we investigated whether chronic optogenetic stimulation of hippocampus CA1 pyramidal neurons in freely moving mice could (a) cause morphological changes reminiscent of homeostatic scaling, (b) modulate synaptic currents that might compensate for chronic excitation, and (c) lead to alterations in Hebbian plasticity. After 24 hr of stimulation with 15‐ms blue light pulses every 90 s, dendritic spine density and area were reduced in the CA1 region of mice expressing channelrhodopsin‐2 (ChR2) when compared to controls. This protocol also reduced the amplitude of mEPSCs for both the AMPA and NMDA components in ex vivo slices obtained from ChR2‐expressing mice immediately after the end of stimulation. Finally, chronic stimulation impaired the induction of LTP and facilitated that of LTD in these slices. Our results indicate that neuronal responses to prolonged network excitation can modulate subsequent Hebbian plasticity in the hippocampus.pt_BR
dc.identifier.citationMOULIN, T. C. et al. Chronic in vivo optogenetic stimulation modulates neuronal excitability, spine morphology, and Hebbian plasticity in the mouse hippocampus. Hippocampus. fev. 2019. doi: https://doi.org/10.1002/hipo.23080pt_BR
dc.identifier.doi10.1002/hipo.23080
dc.identifier.urihttps://repositorio.ufrn.br/jspui/handle/123456789/26810
dc.languageenpt_BR
dc.subjectlong-term depressionpt_BR
dc.subjectlong-term potentiationpt_BR
dc.subjectoptogeneticspt_BR
dc.subjectsynaptic plasticitypt_BR
dc.subjectsynaptic scalingpt_BR
dc.titleChronic in vivo optogenetic stimulation modulates neuronal excitability, spine morphology, and Hebbian plasticity in the mouse hippocampuspt_BR
dc.typearticlept_BR

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