Entry of cholera toxin into polarized human intestinal epithelial cells. Identification of an early brefeldin a sensitive event required for A1-peptide generation

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dc.contributor.authorAlmeida, Jose Bruno de
dc.contributor.authorLencer, W. I.
dc.contributor.authorMoe, S.
dc.contributor.authorStow, Jennifer L.
dc.contributor.authorAusiello, Dennis A.
dc.contributor.authorMadara, J. L.
dc.date.accessioned2023-08-03T19:59:39Z
dc.date.available2023-08-03T19:59:39Z
dc.date.issued1993
dc.description.resumoThe effect of brefeldin-A (BFA), a reversible inhibitor of vesicular transport, on cholera toxin (CT)-induced Cl- secretion (Isc) was examined in the polarized human intestinal cell line, T84. Pretreatment of T84 monolayers with 5 microM BFA reversibly inhibited Isc in response to apical or basolateral addition of 120 nM CT (2.4 +/- 0.5 vs. 68 +/- 3 microA/cm2, n = 5). In contrast, BFA did not inhibit Isc responses to the cAMP agonist VIP (63 +/- 7 microA/cm2). BFA had no effect on cell surface binding and endocytosis of a functional fluorescent CT analog or on the dose dependency of CT induced 32P-NAD ribosylation of Gs alpha in vitro. In contrast, BFA completely inhibited (> 95%) the ability of T84 cells to reduce CT to the enzymatically active A1-peptide. BFA had to be added within the first 10 min of CT exposure to inhibit CT-elicited Isc. The early BFA-sensitive step occurred before a temperature-sensitive step essential for apical CT action. These studies show that sequential steps are required for a biological response to apical CT: (a) binding to cell surfaces and rapid endocytosis; (b) early, BFA-sensitive vesicular transport essential for reduction of the A1-peptide; and (c) subsequent temperature-sensitive translocation of a signal (the A1-peptide or possibly ADP-ribose-Gs alpha) to the basolateral domainpt_BR
dc.identifier.citationALMEIDA, José Bruno de; LENCER, W I; MOE, S; STOW, J L; A AUSIELLO, D; MADARA, J L. Entry of cholera toxin into polarized human intestinal epithelial cells. Identification of an early brefeldin A sensitive event required for A1-peptide generation. Journal Of Clinical Investigation, [S.L.], v. 92, n. 6, p. 2941-2951, 1 dez. 1993. American Society for Clinical Investigation. http://dx.doi.org/10.1172/jci116917. Disponível em: https://www.jci.org/articles/view/116917. Acesso em: 01 ago. 2023.pt_BR
dc.identifier.doi10.1172/JCI116917
dc.identifier.urihttps://repositorio.ufrn.br/handle/123456789/54382
dc.languagept_BRpt_BR
dc.publisherAmerican Society for Clinical Investigationpt_BR
dc.subjectcholera toxinpt_BR
dc.subjectbrefeldin-Apt_BR
dc.subjectintestinal epithelial cellspt_BR
dc.titleEntry of cholera toxin into polarized human intestinal epithelial cells. Identification of an early brefeldin a sensitive event required for A1-peptide generationpt_BR
dc.typearticlept_BR

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