Using cortical neuron markers to target cells in the dorsal cochlear nucleus

Borges, Thawann Malfatti; Boerner, Barbara Ciralli; Hilscher, Markus M; Edwards, Steven J.; Kullander, Klas; Leão, Richardson Naves; Leão, Emelie Katarina Svahn

Using cortical neuron markers to target cells in the dorsal cochlear nucleus

Página do item simplificado Estatísticas

dc.contributor.author	Borges, Thawann Malfatti
dc.contributor.author	Boerner, Barbara Ciralli
dc.contributor.author	Hilscher, Markus M
dc.contributor.author	Edwards, Steven J.
dc.contributor.author	Kullander, Klas
dc.contributor.author	Leão, Richardson Naves
dc.contributor.author	Leão, Emelie Katarina Svahn
dc.date.accessioned	2021-02-19T17:30:47Z
dc.date.available	2021-02-19T17:30:47Z
dc.date.issued	2021-02-09
dc.description.resumo	The dorsal cochlear nucleus (DCN) is a region of particular interest for auditory and tinnitus research. Yet, lack of useful genetic markers for in vivo manipulations hinders elucidation of the DCN contribution to tinnitus pathophysiology. This work assesses whether adeno-associated viral vectors (AAV) containing the calcium/calmodulin-dependent protein kinase 2 alpha (CaMKIIα) promoter and a mouse line of nicotinic acetylcholine receptor alpha 2 subunit (Chrna2)-Cre can target specific DCN populations. We found that CaMKIIα cannot be used to target excitatory fusiform DCN neurons as labelled cells showed diverse morphology indicating they belong to different classes of DCN neurons. Light stimulation after driving Channelrhodopsin2 by the CaMKIIα promoter generated spikes in some units but firing rate decreased when light stimulation coincided with sound. Expression and activation of CaMKIIα-eArchaerhodopsin3.0 in the DCN produced inhibition in some units but sound-driven spikes were delayed by concomitant light stimulation. We explored the existence of Cre+ cells in the DCN of Chrna2-Cre mice by hydrogel embedding technique (CLARITY). There were almost no Cre+ cell bodies in the DCN; however, we identified profuse projections arising from the ventral cochlear nucleus (VCN). Anterograde labeling in the VCN revealed projections to the ipsilateral superior olive and contralateral medial nucleus of the trapezoid body (bushy cells); and a second bundle terminating in the DCN, suggesting the latter to be excitatory Chrna2+ T-stellate cells. Exciting Chrna2+ cells increased DCN firing. This work shows that cortical molecular tools may be useful for manipulating the DCN especially for tinnitus studies	pt_BR
dc.identifier.citation	MALFATTI, Thawann; CIRALLI, Barbara; HILSCHER, Markus M.; EDWARDS, Steven J.; KULLANDER, Klas; LEAO, Richardson N.; LEAO, Katarina E. Using cortical neuron markers to target cells in the dorsal cochlear nucleus. eNeuro, [S. l.], p. ENEURO.0413-20.2020, fev. 2021. Doi: http://dx.doi.org/10.1523/eneuro.0413-20.2020. Disponível em: https://www.eneuro.org/content/early/2021/02/08/ENEURO.0413-20.2020.long. Acesso em: 19 fev. 2021.	pt_BR
dc.identifier.doi	10.1523/eneuro.0413-20.2020
dc.identifier.uri	https://repositorio.ufrn.br/handle/123456789/31566
dc.language	en	pt_BR
dc.publisher	Society for Neuroscience	pt_BR
dc.rights	Attribution 3.0 Brazil	*
dc.rights.uri	http://creativecommons.org/licenses/by/3.0/br/	*
dc.subject	CaMKIIa	pt_BR
dc.subject	Chrna2	pt_BR
dc.subject	Dorsal cochlear nucleus	pt_BR
dc.subject	Extracellular recording	pt_BR
dc.subject	Optogenetics	pt_BR
dc.subject	Ventral cochlear nucleus	pt_BR
dc.title	Using cortical neuron markers to target cells in the dorsal cochlear nucleus	pt_BR
dc.type	article	pt_BR