Specific phospholipids regulate the acquisition of neuronal and astroglial identities in post-mitotic cells

Montaner, Aneley; Santana, Themis Taynah da Silva; Schroeder, Timm; Einiker-Lamas, Marcelo; Girardini, Javier; Costa, Marcos Romualdo; Banchio, Claudia

Specific phospholipids regulate the acquisition of neuronal and astroglial identities in post-mitotic cells

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dc.contributor.author	Montaner, Aneley
dc.contributor.author	Santana, Themis Taynah da Silva
dc.contributor.author	Schroeder, Timm
dc.contributor.author	Einiker-Lamas, Marcelo
dc.contributor.author	Girardini, Javier
dc.contributor.author	Costa, Marcos Romualdo
dc.contributor.author	Banchio, Claudia
dc.date.accessioned	2018-01-12T11:48:21Z
dc.date.available	2018-01-12T11:48:21Z
dc.date.issued	2018-01-11
dc.description.resumo	Hitherto, the known mechanisms underpinning cell-fate specification act on neural progenitors, affecting their commitment to generate neuron or glial cells. Here, we show that particular phospholipids supplemented in the culture media modify the commitment of post-mitotic neural cells in vitro. Phosphatidylcholine (PtdCho)-enriched media enhances neuronal differentiation at the expense of astroglial and unspecified cells. Conversely, phosphatidylethanolamine (PtdEtn) enhances astroglial differentiation and accelerates astrocyte maturation. The ability of phospholipids to modify the fate of post-mitotic cells depends on its presence during a narrow time-window during cell differentiation and it is mediated by the selective activation of particular signaling pathways. While PtdCho-mediated effect on neuronal differentiation depends on cAMP-dependent kinase (PKA)/calcium responsive element binding protein (CREB), PtdEtn stimulates astrogliogenesis through the activation of the MEK/ERK signaling pathway. Collectively, our results provide an additional degree of plasticity in neural cell specification and further support the notion that cell differentiation is a reversible phenomenon. They also contribute to our understanding of neuronal and glial lineage specification in the central nervous system, opening up new avenues to retrieve neurogenic capacity in the brain.	pt_BR
dc.identifier.doi	10.1038/s41598-017-18700-4
dc.identifier.uri	https://repositorio.ufrn.br/jspui/handle/123456789/24537
dc.language	eng	pt_BR
dc.rights	Acesso Aberto	pt_BR
dc.subject	glial cells	pt_BR
dc.subject	post-mitotic cells	pt_BR
dc.subject	phospholipids	pt_BR
dc.subject	cell plasticity	pt_BR
dc.subject	cell biology	pt_BR
dc.title	Specific phospholipids regulate the acquisition of neuronal and astroglial identities in post-mitotic cells	pt_BR
dc.type	article	pt_BR