Estabilidade da glicose "in vitro": comparação entre 4 tipos de tubo de coleta de sangue

Abstract

Introduction: blood glucose determination must be accurate and precise. However, “in vitro” glycolysis may influence the result of this determination. Thus, the ideal is to choose the appropriate collection tube that make it possible to minimize falsely diminished results. Objective: to evaluate the stability of glucose concentration in blood samples collected in tubes with sodium fluoride/EDTA, sodium citrate, clot activator, and clot activator/separator gel over 10h after collection. Material and methods: Blood samples were collected by venipuncture from 36 individuals, after an 8-hour fast. Samples were collected under vacuum, in 4 types of collection tubes and centrifuged within 30 minutes after collection. Glucose dosage was performed every 1 hour, for 10 hours. Results: the samples collected with tubes containing clot activator/without separator gel and tubes with sodium citrate, showed a decay of 10.1% and 7.9%, respectively, 10 hours after collection. No significant decay in glycemia was observed for the other evaluated tubes. Discussion: studies including collection tubes containing sodium citrate in association with other anticoagulants, such as sodium fluoride, EDTA, oxalate, could contribute to assess the best type of collection tube to determinate glycemia. Conclusions: Results suggest that tubes containing clot activator/separating gel and sodium fluoride/EDTA are able to maintain glucose stability “in vitro”, once the sample is centrifuged within 30 minutes of collection.