Lineage Reprogramming of Astroglial Cells from Different Origins into Distinct Neuronal Subtypes

Chouchane, Malek; Faria, Ana Raquel Melo de; Moura, Daniela Maria de Souza; Hilscher, Markus Michael; Schroder, Timm; Leão, Richardson Naves; Costa, Marcos Romualdo

Lineage Reprogramming of Astroglial Cells from Different Origins into Distinct Neuronal Subtypes

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dc.contributor.author	Chouchane, Malek
dc.contributor.author	Faria, Ana Raquel Melo de
dc.contributor.author	Moura, Daniela Maria de Souza
dc.contributor.author	Hilscher, Markus Michael
dc.contributor.author	Schroder, Timm
dc.contributor.author	Leão, Richardson Naves
dc.contributor.author	Costa, Marcos Romualdo
dc.date.accessioned	2017-06-08T16:24:43Z
dc.date.available	2017-06-08T16:24:43Z
dc.date.issued	2017-07
dc.description.resumo	Astroglial cells isolated from the rodent postnatal cerebral cortex are particularly susceptible to lineage reprogramming into neurons. However, it remains unknown whether other astroglial populations retain the same potential. Likewise, little is known about the fate of induced neurons (iNs) in vivo. In this study we addressed these questions using two different astroglial populations isolated from the postnatal brain reprogrammed either with Neurogenin-2 (Neurog2) or Achaete scute homolog-1 (Ascl1). We show that cerebellum (CerebAstro) and cerebral cortex astroglia (CtxAstro) generates iNs with distinctive neurochemical and morphological properties. Both astroglial populations contribute iNs to the olfactory bulb following transplantation in the postnatal and adult mouse subventricular zone. However, only CtxAstro transfected with Neurog2 differentiate into pyramidal-like iNs after transplantation in the postnatal cerebral cortex. Altogether, our data indicate that the origin of the astroglial population and transcription factors used for reprogramming, as well as the region of integration, affect the fate of iNs.	pt_BR
dc.identifier.issn	2213-6711
dc.identifier.uri	https://repositorio.ufrn.br/jspui/handle/123456789/23434
dc.language	eng	pt_BR
dc.rights	Acesso Aberto	pt_BR
dc.rights.uri	An error occurred getting the license - uri.	*
dc.subject	Lineage reprogramming	pt_BR
dc.subject	Induced neurons	pt_BR
dc.subject	Proneural genes	pt_BR
dc.subject	Astroglial cells	pt_BR
dc.subject	Cerebral cortex	pt_BR
dc.subject	Cerebellum	pt_BR
dc.subject	Cell transplantation	pt_BR
dc.title	Lineage Reprogramming of Astroglial Cells from Different Origins into Distinct Neuronal Subtypes	pt_BR
dc.type	article	pt_BR