In vitro anticoagulant and antioxidant activities of Jatropha gossypiifolia L. (Euphorbiaceae) leaves aiming therapeutical applications

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dc.contributor.authorFélix-Silva, Juliana
dc.contributor.authorSouza, Thiago
dc.contributor.authorCâmara, Rafael Barros Gomes da
dc.contributor.authorCabral, Bárbara
dc.contributor.authorSilva-Júnior, Arnóbio Antônio da
dc.contributor.authorRebecchi, Ivanise Marina Moretti
dc.contributor.authorZucolotto, Silvana Maria
dc.contributor.authorRocha, Hugo Alexandre de Oliveira
dc.contributor.authorFernandes-Pedrosa, Matheus de Freitas
dc.date.accessioned2021-01-28T17:15:43Z
dc.date.available2021-01-28T17:15:43Z
dc.date.issued2014-10-20
dc.description.resumoBackground: Jatropha gossypiifolia L. (Euphorbiaceae) is a medicinal plant largely used in folk medicine. Teas from the leaves are popularly used as an antithrombotic agent and the branches are frequently employed as a “thick blood” agent. Considering that the anticoagulant activity associated with antioxidant properties could be beneficial for various cardiovascular diseases, this study’s aim is the evaluation of anticoagulant and antioxidant activities of J. gossypiifolia leaves, seeking new therapeutic purposes for this plant. Methods: The aqueous leaf crude extract (CE) was prepared by decoction and was fractionated by liquid-liquid partition with solvents of increasing polarity. The phytochemical analysis was performed by thin layer chromatography (TLC) and by the spectrophotometric quantification of sugars, proteins and phenolic compounds. The anticoagulant activity was evaluated by prothrombin time (PT) and activated partial thromboplastin time (aPTT) tests. The capacity to act in the fibrinolytic system (fibrinolytic and fibrinogenolytic activities) was also assessed. The antioxidant activity was evaluated by total antioxidant capacity, reducing power, copper chelating activity, iron chelating activity, hydroxyl radical scavenging activity and superoxide radical scavenging assays. The potential toxicity was evaluated using hemolytic assay and the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide (MTT) assay on HEK-293 cells. Results: CE showed significant anticoagulant activity in aPTT test, while no action was observed in PT test, suggesting a preferential action toward the intrinsic and/or common pathway of coagulation. No effect was observed in the fibrinolytic system. Using the aPTT test, it was observed that the residual aqueous (RA) fraction was the most active, being two times more active than CE. RA presented very significant antioxidant activity in all models tested comparable to or even higher than CE. Regarding the safety, CE and RA did not produce significant cytotoxicity in both tests employed. Phytochemical analysis revealed the presence of alkaloids, flavonoids, proteins, tannins, steroids and/or terpenoids and sugars. Conclusions: CE and RA possessed significant anticoagulant and antioxidant activity and absence of cytotoxic effect in vitro, thus showing the potential of the plant, especially RA fraction, as a new source of bioactive molecules for therapeutic purposes, with particular emphasis on the treatment of cardiovascular diseasespt_BR
dc.identifier.citationFÉLIX-SILVA, Juliana; SOUZA, Thiago; CAMARA, Rafael Barros Gomes da; CABRAL, Bárbara; SILVA-JÚNIOR, Arnóbio Antônio; REBECCHI, Ivanise Marina Moretti; ZUCOLOTTO, Silvana Maria; ROCHA, Hugo Alexandre de Oliveira; FERNANDES-PEDROSA, Matheus de Freitas. In Vitro anticoagulant and antioxidant activities of Jatropha gossypiifolia L. (Euphorbiaceae) leaves aiming therapeutical applications. Bmc Complementary And Alternative Medicine, [s. l.], v. 14, n. 1, p. 405, 20 out. 2014. Springer Science and Business Media LLC. Disponível em: https://bmccomplementmedtherapies.biomedcentral.com/articles/10.1186/1472-6882-14-405. Acesso em: 05 ago. 2020. http://dx.doi.org/10.1186/1472-6882-14-405.pt_BR
dc.identifier.doi10.1186/1472-6882-14-405
dc.identifier.issn1472-6882 (online)
dc.identifier.urihttps://repositorio.ufrn.br/handle/123456789/31314
dc.languageenpt_BR
dc.publisherBMCpt_BR
dc.rightsAttribution 3.0 Brazil*
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/br/*
dc.subjectJatropha gossypiifoliapt_BR
dc.subjectEuphorbiaceaept_BR
dc.subject“Pinhão-roxo”pt_BR
dc.subject“Bellyache bush”pt_BR
dc.subjectAnticoagulantpt_BR
dc.subjectAntioxidantpt_BR
dc.subjectCytotoxicitypt_BR
dc.subjectFibrinolyticpt_BR
dc.subjectThin layer chromatographypt_BR
dc.titleIn vitro anticoagulant and antioxidant activities of Jatropha gossypiifolia L. (Euphorbiaceae) leaves aiming therapeutical applicationspt_BR
dc.typearticlept_BR

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