Comprehensive Analysis of Tissue Preservation and Recording Quality from Chronic Multielectrode Implants

Freire, Marco Aurelio M.; Morya, Edgard; Santos, Jose Ronaldo; Guimaraes, Joanilson S.; Lemos, Nelson A. M.; Sameshima, Koichi; Pereira, Antonio; Ribeiro, Sidarta Tollendal Gomes; Nicolelis, Miguel A. L.

Comprehensive Analysis of Tissue Preservation and Recording Quality from Chronic Multielectrode Implants

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dc.contributor.author	Freire, Marco Aurelio M.
dc.contributor.author	Morya, Edgard
dc.contributor.author	Santos, Jose Ronaldo
dc.contributor.author	Guimaraes, Joanilson S.
dc.contributor.author	Lemos, Nelson A. M.
dc.contributor.author	Sameshima, Koichi
dc.contributor.author	Pereira, Antonio
dc.contributor.author	Ribeiro, Sidarta Tollendal Gomes
dc.contributor.author	Nicolelis, Miguel A. L.
dc.date.accessioned	2017-05-24T14:28:11Z
dc.date.available	2017-05-24T14:28:11Z
dc.date.issued	2011
dc.description.resumo	Multielectrodes have been used with great success to simultaneously record the activity of neuronal populations in awake, behaving animals. In particular, there is great promise in the use of this technique to allow the control of neuroprosthetic devices by human patients. However, it is crucial to fully characterize the tissue response to the chronic implants in animal models ahead of the initiation of human clinical trials. Here we evaluated the effects of unilateral multielectrode implants on the motor cortex of rats weekly recorded for 1–6 months using several histological methods to assess metabolic markers, inflammatory response, immediate-early gene (IEG) expression, cytoskeletal integrity and apoptotic profiles. We also investigated the correlations between each of these features and firing rates, to estimate the impact of post-implant time on neuronal recordings. Overall, limited neuronal loss and glial activation were observed on the implanted sites. Reactivity to enzymatic metabolic markers and IEG expression were not significantly different between implanted and non-implanted hemispheres. Multielectrode recordings remained viable for up to 6 months after implantation, and firing rates correlated well to the histochemical and immunohistochemical markers. Altogether, our results indicate that chronic tungsten multielectrode implants do not substantially alter the histological and functional integrity of target sites in the cerebral cortex.	pt_BR
dc.identifier.citation	Freire MAM, Morya E, Faber J, Santos JR, Guimaraes JS, et al. (2011) Comprehensive Analysis of Tissue Preservation and Recording Quality from Chronic Multielectrode Implants. PLoS ONE 6(11): e27554. doi:10.1371/journal.pone.0027554	pt_BR
dc.identifier.issn	1932-6203
dc.identifier.uri	https://repositorio.ufrn.br/jspui/handle/123456789/23063
dc.language	eng	pt_BR
dc.rights	Acesso Aberto	pt_BR
dc.subject	Multielectrode	pt_BR
dc.subject	Implants	pt_BR
dc.subject	chronic tungsten	pt_BR
dc.title	Comprehensive Analysis of Tissue Preservation and Recording Quality from Chronic Multielectrode Implants	pt_BR
dc.type	article	pt_BR