Oxacillin magnetically targeted for the treatment of Methicillin-Resistant S. aureus infection in rats

Carriço, Artur da Silva; Carvalho, Juliana Fernandes; Azevedo, Ítalo Medeiros; Rocha, Keyla Borges Ferreira; Medeiros, Aldo Cunha

Oxacillin magnetically targeted for the treatment of Methicillin-Resistant S. aureus infection in rats

Página do item simplificado Estatísticas

dc.contributor.author	Carriço, Artur da Silva
dc.contributor.author	Carvalho, Juliana Fernandes
dc.contributor.author	Azevedo, Ítalo Medeiros
dc.contributor.author	Rocha, Keyla Borges Ferreira
dc.contributor.author	Medeiros, Aldo Cunha
dc.date.accessioned	2020-04-16T13:51:45Z
dc.date.available	2020-04-16T13:51:45Z
dc.date.issued	2017-01
dc.description.resumo	Purpose: To evaluate the effect of oxacillin bonded to magnetic nanoparticles in local infection model in rat. Methods: Twelve Wistar rats weighing 290±18g were randomly divided into four groups (n=6, each) and all rats had a magnet ring sutured on their right thighs. In the biodistribution group rats 0.1mL of 99mTc-magnetite (0.66 MBq) was injected i.v and after 30 minutes, biodistribution of 99mTc-magnetite was evaluated in right and left thighs. The other groups were inoculated with MRSA in each thigh muscles. Group 1 rats were injected i.v. with magnetite, group 2 with Magnetite + Oxacillin, group 3 with saline twice a day. After 24 hours samples of muscle secretion were harvested for microbiological analysis; muscle, lungs and kidneys for histology. Results: 99mTc-magnetite uptake was three-fold higher in right thigh muscles (with external magnet) than in the left. In magnetite and oxacillin-magnetite groups, bacterial/CFU was significantly lower in thigh muscles than in saline-controls. The inflammatory reaction in muscles and lungs was significantly lower in oxacillin-magnetite group-rats than in other groups (p<0.001) . Conclusion: This study confirms the potential antimicrobial activity of magnetic nanoparticles for Methicillin-Resistant S. aureus strains, which in addition to concentrate the antibiotic at the infection site, positively influenced the treatment.	pt_BR
dc.identifier.citation	CARRIÇO, Artur da Silva et al. Oxacillin magnetically targeted for the treatment of Methicillin-Resistant S. aureus infection in rats. Acta Cirúrgica Brasileira (Online), v. 32, p. 46-55, 2017. ISSN 0102-8650 versão online. DOI https://doi.org/10.1590/s0102-865020170106. Disponível em: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502017000100046&lng=en&tlng=en. Acesso em: 16 abr. 2020.	pt_BR
dc.identifier.doi	https://doi.org/10.1590/s0102-865020170106
dc.identifier.issn	0102-8650
dc.identifier.uri	https://repositorio.ufrn.br/jspui/handle/123456789/28794
dc.language	en	pt_BR
dc.publisher	Acta Cirúrgica Brasileira	pt_BR
dc.subject	Methicillin-Resistant Staphylococcus aureus	pt_BR
dc.subject	Rats	pt_BR
dc.subject	Magnetic Field Therapy	pt_BR
dc.subject	Magnetite Nanoparticles	pt_BR
dc.title	Oxacillin magnetically targeted for the treatment of Methicillin-Resistant S. aureus infection in rats	pt_BR
dc.type	article	pt_BR