Validation of optogenetic protein expression in the Dorsal cochlear nucleus: molecular basis for in vitro and in vivo investigation of tinnitus in mice

Página do item simplificado Estatísticas
dc.contributor.advisorLeão, Emelie Katarina Svahn
dc.contributor.advisorIDpt_BR
dc.contributor.advisorLatteshttp://lattes.cnpq.br/1279823352935722
dc.contributor.authorBorges, Thawann Malfatti
dc.contributor.authorIDpt_BR
dc.contributor.authorLatteshttp://lattes.cnpq.br/1262934391596208
dc.contributor.referees1Leão, Ricardo Maurício Xavier
dc.contributor.referees1IDpt_BR
dc.contributor.referees1Latteshttp://lattes.cnpq.br/8125613125649746
dc.contributor.referees2Leão, Richardson Naves
dc.contributor.referees2IDpt_BR
dc.contributor.referees2Latteshttp://lattes.cnpq.br/0683942077872227
dc.date.accessioned2016-04-29T20:35:41Z
dc.date.available2016-04-29T20:35:41Z
dc.date.issued2015-06-26
dc.description.resumoTinnitus is the perception of a sound in the absence of a corresponding physical stimulus. It is not clear yet what mechanisms are involved in tinnitus and how it starts and/or becomes chronic. Due to the relationship between tinnitus and somatosensory trauma/stimuli, the dorsal cochlear nucleus (DCN), a region known to integrate somatosensory and auditory pathways, has been identified as a potential key structure in the generation of phantom sound perception. Here, we target specific neuronal populations in the DCN to allow further investigation on how this region may contribute to the generation of tinnitus signals that spread to other auditory areas. We examined the expression of optogenetic proteins (Channelrhodopsin 2 - ChR2; and enhanced Archaerhodopsin 3.0 - eArch3.0), targeting neurons expressing Calmoduline Kinase II alpha (CaMKIIa) promoter in wild-type C57/Bl6 mice and neurons expressing nicotinic acetylcholine receptor subunit alpha-2 promoter (ChRNA2) in ChRNA2- Cre transgenic C57/Bl6 mice, using local virus injection, verified by fluorescence microscopy. Unit responses were differentiated based on their electrophysiological response to sound. We then investigated if firing of neurons expressing optogenetic tools can be controlled in vivo and if the same neurons also fire action potentials in response to precisely timed sound stimulation. Both in vivo and preliminary in vitro data shows that neurons expressing ChR2 do respond to sound, and that they furthermore also can respond to light stimulation with a stable and similar waveform. Moreover, in vivo data shows that neurons expressing eArch3.0, responding to sound, will decrease their firing rate when exposed to green light. Thereby showing that optogenetic tools can be used functionally in the DCN, it is possible to further test tinnitus theories by, for example, producing an increased firing rate in the DCN, trying to mimic tinnitus; or inhibiting increased spontaneous firing rate in the DCN of animals with noise-induced or salycilate-induced tinnitus.pt_BR
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)pt_BR
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt_BR
dc.identifier.citationBORGES, Thawann Malfatti. Validation of optogenetic protein expression in the Dorsal cochlear nucleus: molecular basis for in vitro and in vivo investigation of tinnitus in mice. 2015. 60f. Dissertação (Mestrado em Neurociências) - Universidade Federal do Rio Grande do Norte, Natal, 2015.pt_BR
dc.identifier.urihttps://repositorio.ufrn.br/jspui/handle/123456789/20343
dc.language.isoporpt_BR
dc.publisherUniversidade Federal do Rio Grande do Nortept_BR
dc.publisher.countryBrasilpt_BR
dc.publisher.initialsUFRNpt_BR
dc.publisher.programPROGRAMA DE PÓS-GRADUAÇÃO EM NEUROCIÊNCIASpt_BR
dc.rightsAcesso Abertopt_BR
dc.subjectOptogeneticspt_BR
dc.subjectDorsal cochlear nucleuspt_BR
dc.subjectAuditory systempt_BR
dc.subjectTinnituspt_BR
dc.subject.cnpqCNPQ::OUTROS::CIENCIAS: NEUROCIÊNCIASpt_BR
dc.titleValidation of optogenetic protein expression in the Dorsal cochlear nucleus: molecular basis for in vitro and in vivo investigation of tinnitus in micept_BR
dc.title.alternativeValidação da expressão de proteínas optogenéticas no Núcleo coclear dorsal: bases moleculares para investigação in vitro e in vivo de tinnitus em camundongospt_BR
dc.typemasterThesispt_BR

Arquivos

Pacote Original

Agora exibindo 1 - 1 de 1
Imagem de Miniatura
Nome:
ThawannMalfattiBorges_DISSERT.pdf
Tamanho:
24.01 MB
Formato:
Adobe Portable Document Format
Imagem de Miniatura
 Baixar

Coleções

PPGNEURO - Mestrado em Neurociências
Repositório Institucional - UFRN Campus Universitário Lagoa NovaCEP 59078-970 Caixa postal 1524 Natal/RN - BrasilUniversidade Federal do Rio Grande do Norte© Copyright 2025. Todos os direitos reservados.
Contato+55 (84) 3342-2260 - R232Setor de Repositórios Digitaisrepositorio@bczm.ufrn.br
DSpaceIBICT
OasisBR
LAReferencia
Customizado pela CAT - BCZM