An insulin receptor-binding multifunctional protein from tamarindus indica L. presents a hypoglycemic effect in a diet-induced type 2 diabetes-preclinical study

dc.contributor.authorMaciel, Bruna Leal Lima
dc.contributor.authorCosta, Izael de Sousa
dc.contributor.authorLima, Mayara Santos Rosa
dc.contributor.authorMedeiros, Amanda
dc.contributor.authorBezerra, Lucas Lima
dc.contributor.authorSantos, Paula
dc.contributor.authorSerquiz, Alexandre
dc.contributor.authorLima, Maíra Conceição Jerônimo de Souza
dc.contributor.authorOliveira, Gerciane Santos de
dc.contributor.authorSantos, Elizeu Antunes dos
dc.contributor.authorMonteiro, Norberto de Kássio Vieira
dc.contributor.authorMorais, Ana Heloneida
dc.date.accessioned2024-01-30T13:21:46Z
dc.date.available2024-01-30T13:21:46Z
dc.date.issued2022-07
dc.description.resumoThe objectives of this study were to evaluate the hypoglycemic effect of the trypsin inhibitor isolated from tamarind seeds (TTI) in an experimental model of T2DM and the in silico interaction between the conformational models of TTI 56/287 and the insulin receptor (IR). After inducing T2DM, 15 male Wistar rats were randomly allocated in three groups (n = 5): 1—T2DM group without treatment; 2—T2DM group treated with adequate diet; and 3—T2DM treated with TTI (25 mg/kg), for 10 days. Insulinemia and fasting glucose were analyzed, and the HOMA-IR and HOMA-β were calculated. The group of animals treated with TTI presented both lower fasting glucose concentrations (p = 0.0031) and lower HOMA-IR indexes (p = 0.0432), along with higher HOMA-β indexes (p = 0.0052), than the animals in the other groups. The in silico analyses showed that there was an interaction between TTIp 56/287 and IR with interaction potential energy (IPE) of −1591.54 kJ mol−1 (±234.90), being lower than that presented by insulin and IR: −894.98 kJ mol−1 (±32.16). In addition, the presence of amino acids, type of binding and place of interaction other than insulin were identified. This study revealed the hypoglycemic effect of a bioactive molecule of protein origin from Tamarind seeds in a preclinical model of T2DM. Furthermore, the in silico analysis allowed the prediction of its binding in the IR, raising a new perspective for explaining TTI’s action on the glycemic responsept_BR
dc.identifier.citationCOSTA, Izael de Sousa; LIMA, Mayara Santos Rosa; MEDEIROS, Amanda; BEZERRA, Lucas Lima; SANTOS, Paula; SERQUIZ, Alexandre; LIMA, Maíra Conceição Jerônimo de Souza; OLIVEIRA, Gerciane Santos de; SANTOS, Elizeu Antunes dos; MACIEL, Bruna Leal Lima; MONTEIRO, Norberto de Kássio Vieira; MORAIS, Ana Heloneida. An insulin receptor-binding multifunctional protein from tamarindus indica L. presents a hypoglycemic effect in a diet-induced type 2 diabetes-preclinical study. Foods, v. 11, p. 2207, 2022. 25 jul. 2022. DOI: 10.3390/foods11152207. Disponível em: https://www.mdpi.com/2304-8158/11/15/2207. Acesso em: 25 jan. 2024.pt_BR
dc.identifier.doihttp://dx.doi.org/10.3390/foods11152207
dc.identifier.urihttps://repositorio.ufrn.br/handle/123456789/57445
dc.languageenpt_BR
dc.publisherFoodspt_BR
dc.rightsAttribution 3.0 Brazil*
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/br/*
dc.subjectTrypsin inhibitorpt_BR
dc.subjectTamarindpt_BR
dc.subjectHypoglycemic agentpt_BR
dc.subjectPlant proteinspt_BR
dc.subjectMolecular docking simulationpt_BR
dc.titleAn insulin receptor-binding multifunctional protein from tamarindus indica L. presents a hypoglycemic effect in a diet-induced type 2 diabetes-preclinical studypt_BR
dc.typearticlept_BR

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