Imageamento de cálcio em fatias frescas de cérebro para avaliação de alterações na atividade de neurônios piramidais do córtex auditivo primário de camundongos expostos ao ruído

Abstract

Tinnitus is sound phantom perception in the absence of external stimuli. In tinnitus animal models, hyperactivity and increased synchrony are reported in the dorsal cochlear nucleus, inferior colliculus, medial geniculate nucleus and the primary auditory cortex. However, which neuronal classes in the primary auditory cortex (A1) are more affected by noise-exposure remains to be described. A1 is a putative target for treatments involving neuronal activity modulation. Using a genetically encoded calcium indicator (GCaMP6f), we analyzed the effects of noise exposure on the activity of positive CamKIIα excitatory cells in the infragranular layers (5-6) of noise-exposed mice.