Insulin modulates myogenesis and muscle atrophy resulting from skin scald burn in young male rats

Quintana, Hananiah Tardivo; Baptista, Vivianne Izabelle de Araújo; Lazzarin, Mariana Cruz; Antunes, Hanna Karen Moreira; Le Sueur-Maluf, Luciana; Oliveira, Camila Aparecida Machado de; Oliveira, Flavia de

Insulin modulates myogenesis and muscle atrophy resulting from skin scald burn in young male rats

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dc.contributor.author	Quintana, Hananiah Tardivo
dc.contributor.author	Baptista, Vivianne Izabelle de Araújo
dc.contributor.author	Lazzarin, Mariana Cruz
dc.contributor.author	Antunes, Hanna Karen Moreira
dc.contributor.author	Le Sueur-Maluf, Luciana
dc.contributor.author	Oliveira, Camila Aparecida Machado de
dc.contributor.author	Oliveira, Flavia de
dc.date.accessioned	2020-12-17T14:01:29Z
dc.date.available	2020-12-17T14:01:29Z
dc.date.issued	2020-08-17
dc.description.resumo	Burn injuries (BIs) due to scalding are one of the most common accidents among children. BIs greater than 40% of total body surface area are considered extensive and result in local and systemic response. We sought to assess morphological and myogenic mechanisms through both short- and long-term intensive insulin therapies that affect the skeletal muscle after extensive skin BI in young rats. Materials and methods: Wistar rats aged 21 d were distributed into four groups: control (C), control with insulin (C þ I), scald burn injury (SI), and SI with insulin (SI þ I). The SI groups were submitted to a 45% total body surface area burn, and the C þ I and SI þ I groups received insulin (5 UI/Kg/d) for 4 or 14 d. Glucose tolerance and the homeostatic model assessment of insulin resistance index were determined. Gastrocnemius muscles were analyzed for histopathological, morphometric, and immunohistochemical myogenic parameters (Pax7, MyoD, and MyoG); in addition, the expression of genes related to muscle atrophy (MuRF1 and MAFbx) and its regulation (IGF-1) were also assessed. Results: Short-term treatment with insulin favored muscle regeneration by primary myogenesis and decreased muscle atrophy in animals with BIs, whereas the long-term treatment modulated myogenesis by increasing the MyoD protein. Both treatments improved histopathological parameters and secondary myogenesis by increasing the MyoG protein. Conclusions: Treatment with insulin benefits myogenic parameters during regeneration and modulates MuRF1, an important mediator of muscle atrophy	pt_BR
dc.identifier.citation	QUINTANA, Hananiah Tardivo; BAPTISTA, Vivianne Izabelle de Araújo; LAZZARIN, Mariana Cruz; ANTUNES, Hanna Karen Moreira; LE SUEUR-MALUF, Luciana; OLIVEIRA, Camila Aparecida Machado de; OLIVEIRA, Flavia de. Insulin modulates myogenesis and muscle atrophy resulting from skin scald burn in young male rats. Journal Of Surgical Research, [s. l.], v. 257, p. 56-68, jan. 2021. Disponível em: https://www.journalofsurgicalresearch.com/article/S0022-4804(20)30497-2/fulltext. Acesso em: 03 nov. 2020. http://dx.doi.org/10.1016/j.jss.2020.07.040	pt_BR
dc.identifier.doi	10.1016/j.jss.2020.07.040
dc.identifier.issn	0022-4804
dc.identifier.issn	1095-8673
dc.identifier.uri	https://repositorio.ufrn.br/handle/123456789/31035
dc.language	en	pt_BR
dc.publisher	Elsevier	pt_BR
dc.rights	Attribution 3.0 Brazil	*
dc.rights.uri	http://creativecommons.org/licenses/by/3.0/br/	*
dc.subject	Burn injury	pt_BR
dc.subject	Insulin	pt_BR
dc.subject	Skeletal muscle	pt_BR
dc.subject	Myogenesis	pt_BR
dc.subject	Atrophy	pt_BR
dc.title	Insulin modulates myogenesis and muscle atrophy resulting from skin scald burn in young male rats	pt_BR
dc.type	article	pt_BR