A low-molecular-weight galactofucan from the seaweed, Spatoglossum schröederi, binds fibronectin and inhibits capillary-like tube formation in vitro

Menezes, Maira Maria; Nobre, Leonardo Thiago Duarte Barreto; Rossi, Gustavo Rodrigues; Almeida-Lima, Jailma; Melo-Silveira, Raniere Fagundes; Franco, Celia Regina Cavichiolo; Trindade, Edvaldo Silva; Nader, Helena Bonciani; Rocha, Hugo Alexandre de Oliveira

A low-molecular-weight galactofucan from the seaweed, Spatoglossum schröederi, binds fibronectin and inhibits capillary-like tube formation in vitro

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dc.contributor.author	Menezes, Maira Maria
dc.contributor.author	Nobre, Leonardo Thiago Duarte Barreto
dc.contributor.author	Rossi, Gustavo Rodrigues
dc.contributor.author	Almeida-Lima, Jailma
dc.contributor.author	Melo-Silveira, Raniere Fagundes
dc.contributor.author	Franco, Celia Regina Cavichiolo
dc.contributor.author	Trindade, Edvaldo Silva
dc.contributor.author	Nader, Helena Bonciani
dc.contributor.author	Rocha, Hugo Alexandre de Oliveira
dc.date.accessioned	2020-12-30T13:37:13Z
dc.date.available	2020-12-30T13:37:13Z
dc.date.issued	2018-05
dc.description.resumo	A low-molecular-weight (LMW) heterofucan (designated fucan B) was obtained from the brown seaweed, Spatoglossum schröederi, and its activity as an inhibitor of capillary-like tube formation by endothelial cells (ECs) was analyzed. Chemical, infrared and electrophoretic analyses confirmed the identity of fucan B. In contrast to other LMW fucans, fucan B (0.012–0.1 mg/mL) inhibited ECs capillary-like tube formation in a concentrationdependent manner. In addition, fucan B (0.01–0.05 mg/mL) did not affect ECs proliferation. Fucan B also inhibited ECs migration on a fibronectin-coated surface, but not on laminin- or collagen-coated surfaces. Biotinylated fucan B was used as a probe to identify its localization. Confocal microscopy experiments revealed that biotinylated fucan did not bind to the cell surface, but rather only to fibronectin. Our findings suggest that fucan B inhibits ECs capillary-like tube formation and migration by binding directly to fibronectin and blocking fibronectin sites recognized by cell surface ligands. However, further studies are needed to evaluate the in vivo effects of fucan B	pt_BR
dc.identifier.citation	MENEZES, Maira Maria; NOBRE, Leonardo Thiago Duarte Barreto; ROSSI, Gustavo Rodrigues; ALMEIDA-LIMA, Jailma; MELO-SILVEIRA, Raniere Fagundes; FRANCO, Celia Regina Cavichiolo; TRINDADE, Edvaldo Silva; NADER, Helena Bonciani; ROCHA, Hugo Alexandre de Oliveira. A low-molecular-weight galactofucan from the seaweed, Spatoglossum schröederi, binds fibronectin and inhibits capillary-like tube formation in vitro. International Journal Of Biological Macromolecules, [s. l.], v. 111, p. 1067-1075, maio 2018. Elsevier BV. Disponível em: https://www.sciencedirect.com/science/article/pii/S0141813017333792?via%3Dihub. Acesso em: 18 set. 2020.http://dx.doi.org/10.1016/j.ijbiomac.2018.01.119.	pt_BR
dc.identifier.doi	10.1016/j.ijbiomac.2018.01.119
dc.identifier.issn	0141-8130 (print)
dc.identifier.uri	https://repositorio.ufrn.br/handle/123456789/31159
dc.language	en	pt_BR
dc.publisher	Elsevier	pt_BR
dc.rights	Attribution 3.0 Brazil	*
dc.rights.uri	http://creativecommons.org/licenses/by/3.0/br/	*
dc.subject	Fucoidan	pt_BR
dc.subject	Brown seaweed, endothelial cells	pt_BR
dc.subject	Anti-angiogenic	pt_BR
dc.title	A low-molecular-weight galactofucan from the seaweed, Spatoglossum schröederi, binds fibronectin and inhibits capillary-like tube formation in vitro	pt_BR
dc.type	article	pt_BR