DBQ - Departamento de Bioquímica

URI Permanente desta comunidadehttps://repositorio.ufrn.br/handle/1/6

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Heterofucan from Sargassum filipendula induces apoptosis in HeLa cells
(MDPI, 2011-04-14) Costa, Leandro Silva; Telles, Cinthia Beatrice Silva; Oliveira, Ruth Medeiros; Nobre, Leonardo Thiago Duarte Barreto; Dantas-Santos, Nednaldo; Câmara, Rafael Barros Gomes da; Costa, Mariana Santana Santos Pereira; Almeida-Lima, Jailma; Melo-Silveira, Raniere Fagundes; Albuquerque, Ivan Rui Lopes; Leite, Edda Lisboa; Rocha, Hugo Alexandre de Oliveira
Fucan is a term used to denominate a family of sulfated polysaccharides rich in sulfated L-fucose. Heterofucan SF-1.5v was extracted from the brown seaweed Sargassum filipendula by proteolytic digestion followed by sequential acetone precipitation. This fucan showed antiproliferative activity on Hela cells and induced apoptosis. However, SF-1.5v was not able to activate caspases. Moreover, SF-1.5v induced glycogen synthase kinase (GSK) activation, but this protein is not involved in the heterofucan SF-1.5v induced apoptosis mechanism. In addition, ERK, p38, p53, pAKT and NFκB were not affected by the presence of SF-1.5v. We determined that SF-1.5v induces apoptosis in HeLa mainly by mitochondrial release of apoptosis-inducing factor (AIF) into cytosol. In addition, SF-1.5v decreases the expression of anti-apoptotic protein Bcl-2 and increased expression of apoptogenic protein Bax. These results are significant in that they provide a mechanistic framework for further exploring the use of SF-1.5v as a novel chemotherapeutics against human cervical cancer

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Navegando DBQ - Departamento de Bioquímica por Assunto "Apoptosis-inducing factor (AIF)"