Navegando por Autor "Santos, Yago Queiroz dos"

Agora exibindo 1 - 8 de 8

Antitryptical, anticoagulant and hemagglutinating activities of Eucalyptus sp. seeds
(F1000Research, 2019-01-08) Santos, Yago Queiroz dos; Carelli, Gabriella Silva Campos; Veras, Bruno Oliveira de; Batista, Virgínia Cunha; França, Anderson Felipe Jácome de; Silva, Márcia Vanusa da; Santos, Elizeu Antunes dos
Background: Plant biodiversity has great value for science being an inexhaustible source for new bioactive molecules capable of offering environmentally friendly and innovative solutions for various areas of the industry. The scientific community has increased their interest in the study of plant species in the search of new molecules and to determine their mechanisms of action. Plant seeds are natural sources of bioactive compounds, such as carbohydrates, lipids and proteins with special focus on enzymatic inhibitors which protect them against digestive enzymes of phytopathogens and lectins that play an important role on carbohydrate signalization and metabolism during germination. The objective of the present study was to evaluate and describe the protein profile and to test the hemagglutinating, hemolytic and anticoagulant activities, as well as the antitryptic effect of extracts and fractions obtained from seeds of Eucalyptus species. Methods: The crude protein extract was obtained from the seed of Eucalyptus sp. with 0.02 M sodium phosphate buffer, at pH 6.6, and fractionated using ammonium sulfate in order to study its antitryptical properties as well as the capacity of hemagglutination and influence on hemostasis. Results: The crude extract showed a high effectiveness for trypsin inhibition. For hemagglutinating activity, the ammonium sulfate fraction 0-30% presented better activity, while no hemolytic activity was present in the obtained fractions. For anticoagulation assay, the fraction 0-30% showed better results. Conclusions: Taken together, the obtained results demonstrate the biotechnological potential of Eucalyptus sp. seeds, although further study is still necessary to better isolate as well as describe the bioactive compounds
Evaluation of heterologous protein expression at different concentrations of MGSO4 and IPTG in Escherichia Coli W110
(Atena Editora, 2019) Santos, Yago Queiroz dos; Carelli, Gabriella Silva Campos; Veras, Bruno Oliveira de; Cruz, Joelton Igor Oliveira da; Moura, Geovanna Maria Medeiros; Marques Neto, Antônio Moreira; França, Anderson Felipe Jácome de
Since the improvement of gene sequencing techniques, the expression of heterologous proteins is gaining increasing relevance given the possibility of large-scale production when compared to the isolation of these same molecules in their original sources. However, heterologous expression systems continue to require for a suitable induction relevant amounts of synthetic molecules like IPTG (Isopropyl β-D-1-thiogalactopyranoside) which have toxicity and cost limitation when used in large scale. Thus, the objective of this work was to evaluate the effects of bivalent salts such as MgSO4 in association with IPTG on heterologous expression system pDMI.1 for a possible better cost-benefit proposal in the current heterologous protein expression protocol. Magnesium Sulfate contributes to an increase in bacterial physiology as a whole, inducing a higher protein expression acting as an indirect and alternative inducer in these conditions, with the advantages of presenting low cost and low cellular toxicity. Based on these obtained data, we propose the usage of low MgSO4 molar concentrations in current heterologous protein expression protocols performed in our group for the pDMI.1 vector. Additional studies are still needed in order to evaluate the effects of this salt on different expression systems at wide range of microorganisms
Otimização da produção e modelagem in silico de celulase de uma linhagem marinha de Bacillus subtilis halotolerante
(Universidade Federal do Rio Grande do Norte, 2020-05-08) Santos, Yago Queiroz dos; Santos, Elizeu Antunes dos; ; http://lattes.cnpq.br/6762251930590306; ; http://lattes.cnpq.br/6854116205386919; Uchoa, Adriana Ferreira; ; http://lattes.cnpq.br/6644671747055211; Santos, Everaldo Silvino dos; ; http://lattes.cnpq.br/4330639792072559; Macedo, Gorete Ribeiro de; ; http://lattes.cnpq.br/3324083094904117; Rabêlo, Luciana Maria Araújo; ; http://lattes.cnpq.br/2165223941043909; Santos, Paula Ivani Medeiros dos; ; http://lattes.cnpq.br/1631947914918841
A demanda atual por fontes de energias renováveis e ecológicas impulsionou a busca de alternativas capazes de substituir o uso de combustíveis fósseis. Uma das inovações mais promissoras para impactar positivamente o cenário mundial de energia é a produção de bioetanol de segunda geração (2Getanol) a partir de açúcares redutores derivados da degradação enzimática de material lignocelulósico que normalmente é descartada em processos agroindustriais. A celulose é o homopolissacarídeo linear mais abundante do planeta formado por unidades de glicose ligadas por ligações glicosídicas do tipo β-(1-4). O presente trabalho teve por objetivo a modelagem estrutural e o estabelecimento de bioprocessos enzimáticos otimizados para a conversão do material lignocelulósico processado em açúcares redutores fermentescíveis. Após o processo de otimização, o máximo rendimento prático obtido foi 7,30% superior à condição inicial, atingindo atividade enzimática de 318,809 ± 0,784 U/mL enquanto a modelagem in silico evidenciou a presença de estrutura monomérica na principal endoglucanase envolvida no processo. De acordo com os dados obtidos neste trabalho, foi possível concluir a possibilidade aplicação, deste bioprocesso otimizado, nos processos industriais para geração de bioetanol.
Phylogenetic analysis and identification of a cellulase producing Bacillus Sp. Strain by 16s RRNA sequencing
(Atena Editora, 2019) Santos, Yago Queiroz dos; França, Anderson Felipe Jácome de; Veras, Bruno Oliveira de; Carelli, Gabriella Silva Campos; Moura, Geovanna Maria Medeiros; Cruz, Joelton Igor Oliveira da; Oliveira, Fernanda Granja da Silva; Oliveira, João Ricardhis Saturnino de; Amorim, Luciclaudio Cassimiro de; Santos, Elizeu Antunes dos
The microorganisms belonging to genus Bacillus include a wide spectra and ubiquitous group of bacteria that can be found from the forest soil, to marine ecosystems occurring in association with a variety of aquatic organisms such as scleractinian corals present in the intertidal boulders. Therefore, these microorganisms are exposed to various abiotic stresses that cause ecological selection for a physiologically adapted microbiota to such extremes of temperature and salinity. In this work, we isolated and characterized a cellulase from a marine bacterial strain and indentified its specie by 16S rRNA sequencing followed by a BLAST analysis. The cellulolytic strain called SR22 showed to be a gram-positive spore-forming bacilli, facultative anaerobe, and catalase positive, as well as negative for indole, H2S production, and citrate utilization; those findings led us to consider the isolate belonging to the genus Bacillus, which was confirmed by the phylogenetic analysis, which revealed that the SR22 strain formed a clade with Bacillus subtilis. Its nucleotide sequence was deposited in GenBank as Accession No. MH119099 and the degree of sequence similarity of strain SR22 to Bacillus sp. was 99%. Taken together, the present data indicate the present celulase-positive indentified strain as a potential and useful candidate for industrial applications that employs celulase degrading processes like second-generation bioethanol and paper industries being still necessary further studies to complete characterize this microorganism secretome
Potential pharmacological applications of lectins
(Atena Editora, 2020) Cruz, Joelton Igor Oliveira da; Rabêlo, Luciana Maria Araújo; França, Anderson Felipe Jácome de; Veras, Bruno Oliveira de; Santos, Yago Queiroz dos; Moura, Geovanna Maria de Medeiros; Marques Neto, Antônio Moreira; Lima, Rayana Vanessa da Costa; Carelli, Gabriella Silva Campos
The word lectin is derived from the Latin legere which means “to choose” or “to select” and was introduced in biochemistry in the second half of the 20th century. Such molecules are characterized as glycoproteins that generally do not have an enzymatic role while have at least one non-catalytic domain − lectins are reversibly and specifically associated with simple and complex carbohydrates. Lectins differ from all other proteins through this type of connection with carbohydrates, promoting the ability to agglutinate cells through interaction with specific glycids having a non-immune origin. The present work aims to review the specialized literature in order to list the potential pharmacological applications derived from lectin-carbohydrate specificity in its most varied approaches
Produção, purificação e caracterização de uma celulase termostável e halotolerante de uma linhagem marinha de Bacillus sp.
(2016-06-16) Santos, Yago Queiroz dos; Santos, Elizeu Antunes dos; Lira, Krystyna Gorlach; ; http://lattes.cnpq.br/0736700530533471; ; http://lattes.cnpq.br/6762251930590306; ; http://lattes.cnpq.br/6854116205386919; Rodrigues, Cicera Raquel Fernandes; ; http://lattes.cnpq.br/8398785862122333; Melo, Maria Celeste Nunes de; ; http://lattes.cnpq.br/0580551464788795; Theodoro, Raquel Cordeiro; ; http://lattes.cnpq.br/0977453259767928
A atual demanda por fontes de energia renováveis tem impulsionado a busca de alternativas capazes de substituir o uso de combustíveis fósseis. Uma das inovações mais promissoras para impactar positivamente o cenário mundial de energia é a produção de bioetanol de segunda geração (B2G), derivado de monossacarídeos obtidos a partir da degradação enzimática de material lignocelulósico que normalmente é descartado nos atuais processos agroindustriais. No presente trabalho, uma celulase halotolerante secretada por uma linhagem marinha Bacillus sp. SR22 com grande resistência às mudanças de temperatura e pH foi isolada e caracterizada. A endoglucanase de massa aproximada de 37.35 kDa nomeada como Bc22Cel foi purificada por precipitação de sulfato de amónio, cromatografia de gel filtração e extração do gel após eletroforese em gel de poliacrilamida em condições nativas. O valor ideal de pH e temperatura de Bc22Cel foram 6,5 e 60 ° C, respectivamente. A enzima purificada demonstrou considerável propriedade halofílica ao manter mais de 70% de atividade residual mesmo quando pré-incubadas com 1,5 M de NaCl durante 1 hora. Após a análise cinética de enzima purificada os valores de Km e Vmax foram estabelecidos em 0,3953 nmol.ml-1 e 0,0167 μmol.ml-1.min-1, respectivamente. Avaliados conjuntamente, os presentes dados apontam a Bc22Cel como uma candidata potencial para aplicações industriais de degradação de celulose.
Proteases and their inhibitors in coagulation and inflammation
(Atena Editora, 2020) Santos, Jorge Anderson Nascimento dos; Marques Neto, Antônio Moreira; França, Anderson Felipe Jácome de; Santos, Yago Queiroz dos; Carelli, Gabriella Silva Campos; Cruz, Joelton Igor Oliveira da; Rabêlo, Luciana Maria Araújo; Veras, Bruno Oliveira de; Moura, Geovanna Maria de Medeiros
Proteases or peptidases are molecules that promote cleavage through the hydrolysis of peptide bonds present in proteins and polypeptides, transforming them into smaller amino acid or polypeptide residues. The group of serine proteases is predominant in peptidases and is found in almost all living organisms, constituting the family of proteases best characterized and physiologically versatile. Within the serine proteases are protease inhibitors, proteins capable of blocking and / or inhibiting the catalytic activity of proteolytic enzymes, being found naturally in most living organisms. In serine protease inhibitors, two distinct categories have already been classified, entrapment inhibitors and high affinity inhibitors. The hemostatic system participates in maintaining balance in living organisms, maintaining adequate blood pressure and perfusion, controlling bleeding caused by damage to blood vessels, through processes known as coagulation, where most factors in the coagulation cascade are serine proteases or cofactors . Among the most important serine proteases that act in the coagulation cascade are some coagulation factors (II, VII, IX, X, XI, XII, for example), the vasodilating molecule kallikrein, which is also a serine protease that acts on various substrates releasing vasoactive peptides, exercising their natural functions. In disorders in this cascade, medicine uses inhibitors with anticoagulant action, managing to reverse or inhibit these diseases. In inflammatory reactions, which is an essential step for controlling microbial invasion or tissue damage, as well as for maintaining tissue homeostasis, neutrophils secrete serine proteases such as neutrophil elastase (NE1), proteinase 3 (PR3) and cathepsin G ( CG), which are components of one of the most important molecular arsenals for the defense of the organism. Therefore, these facts suggest that new studies in this area are of great relevance to the evolution of science and medicine
Screening of L-Asparaginase the salt-tolerant and thermostable marine Bacillus Subtilis Strain SR61
(Atena Editora, 2019) Veras, Bruno Oliveira de; Santos, Yago Queiroz dos; França, Anderson Felipe Jácome de; Ribeiro, Penha Patricia Cabral; Barbosa, Elaine Costa Almeida; Gorlach-Lira, Krystyna
The marine environment harbors different microorganisms that inhabit niches with adverse conditions, such as variation of temperature, pressure and salinity. To survive these particular conditions, marine bacteria use unique metabolic and biochemical characteristics, producing enzymes that have high industrial value. The objective of this study was to observe the production of thermostable and halotolerant L-asparaginase by marine bacteria isolated from the coral reefs of Cabo Branco, State of Paraíba, Brazil. An L-asparaginase-producing bacterial isolate was identified and identified by phylogenetic analysis as Bacillus subtilis SR61, via a 16S ribosomal RNA assay. For screening of L-asparaginase by SR61, it was inoculated in a differential medium to induce the production of salt tolerant extracellular thermostable enzyme with the addition of increasing salt concentrations (0, 0.5, 1.0, 1.5 e 2.0 M NaCl) by 55 0C for 24 hours. The screening showed a capacity of halotolerant and thermostable L-asparaginase production by the isolate identified as Bacillus subtilis, the production being limited to 1.0 M salt, having a total activity (IU / mL) 231.4 ± 3.57 and specifying IU / μg 8.39. Bacillus subtilis SR61 was able to produce L-asparaginase when submitted to a high salinity environment, demonstrating the halophytic nature of the isolate, having several applications in several industrial branches

SIGAA

Navegando por Autor "Santos, Yago Queiroz dos"