Navegando por Autor "Santos, Joaquim Gasparini dos"
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Artigo JMJD1A, H3K9me1, H3K9me2 and ADM expression as prognostic markers in oral and oropharyngeal squamous cell carcinoma(Public Library of Science, 2018-03-28) Maia, Lucas de Lima; Peterle, Gabriela Tonini; Santos, Marcelo dos; Trivilin, Leonardo Oliveira; Mendes, Suzanny Oliveira; Oliveira, Mayara Mota de; Santos, Joaquim Gasparini dos; Stur, Elaine; Agostini, Lidiane Pignaton; Couto, Cinthia Vidal Monteiro da Silva; Dalbo, Juliana; Assis, Arícia Leone Evangelista Monteiro de; Archanjo, Anderson Barros; Mercante, Ana Maria Da Cunha; Lopez, Rossana Veronica Mendoza; Nunes, Fábio Daumas; Carvalho, Marcos Brasilino de; Tajara, Eloiza Helena; Louro, Iúri Drumond; Álvares-da-Silva, Adriana MadeiraAims: Jumonji Domain-Containing 1A (JMJD1A) protein promotes demethylation of histones, especially at lysin-9 of di-methylated histone H3 (H3K9me2) or mono-methylated (H3K9me1). Increased levels of H3 histone methylation at lysin-9 (H3K9) is related to tumor suppressor gene silencing. JMJD1A gene target Adrenomeduline (ADM) has shown to promote cell growth and tumorigenesis. JMJD1A and ADM expression, as well as H3K9 methylation level have been related with development risk and prognosis of several tumor types. Methods and results: We aimed to evaluate JMJD1A, ADM, H3K9me1 and H3K9me2expression in paraffinembedded tissue microarrays from 84 oral and oropharyngeal squamous cell carcinoma samples through immunohistochemistry analysis. Our results showed that nuclear JMJD1A expression was related to lymph node metastasis risk. In addition, JMJD1A cytoplasmic expression was an independent risk marker for advanced tumor stages. H3K9me1 cytoplasmic expression was associated with reduced disease-specific death risk. Furthermore, high H3K9me2 nuclear expression was associated with worse specific-disease and disease-free survival. Finally, high ADM cytoplasmic expression was an independent marker of lymph node metastasis risk. Conclusion: JMJD1A, H3K9me1/2 and ADM expression may be predictor markers of progression and prognosis in oral and oropharynx cancer patients, as well as putative therapeutic targetsArtigo PAI-1 expression in intratumoral inflammatory infiltrate contributes to lymph node metastasis in oral cancer: a cross-sectional study(Elsevier, 2021-04-15) Oliveira, Mayara Mota de; Peterle, Gabriela Tonini; Couto, Cinthia Vidal Monteiro da Silva; Maia, Lucas de Lima; Kühl, Andre; Santos, Joaquim Gasparini dos; Moysés, Raquel Ajub; Trivilin, Leonardo Oliveira; Borçoi, Aline Ribeiro; Archanjo, Anderson Barros; Assis, Arícia Leone Evangelista Monteiro de; Nunes, Fábio Daumas; Santos, Marcelo dos; Silva, Adriana Madeira Álvares daIntroduction: Immune cells contribute with mediators in the protein expression profile of the tumor microenvi ronment. Levels of plasminogen activator inhibitor-1 (PAI-1) are elevated in non-malignant inflammatory con ditions; however, the association between PAI-1 expression and inflammation remains uncertain in oral squamous cell carcinoma (OSCC). This study aimed to investigate PAI-1 expression in mononuclear inflammatory cell infiltrate in OSCC and its role as a prognostic marker. Methods: Samples were collected from patients with OSCC, treated surgically, and followed for 24 months after the procedure. Thirty-nine tumoral tissue were analyzed using immunohistochemistry. Correlation between protein expression, clinicopathological parameters, and the prognosis was investigated. Results: Positive PAI-1 expression in mononuclear inflammatory cell infiltrate was significantly associated with lymph node status (p = 0.009) and with the cytoplasmic expression of vascular endothelial growth factor A (VEGFA) (p = 0.028). Multivariate analysis revealed weak PAI-1 expression as an independent marker for lymph node metastases, with approximately 8-fold increased risk compared to strong expression (OR = 8.60; CI =1.54–48.08; p = 0.014). Conclusion: Our results suggest that the strong PAI-1 expression in intratumoral inflammatory infiltrate is an indicator of a better prognosis for patients diagnosed with oral squamous cell carcinoma.Artigo PAI-1, CAIX, and VEGFA expressions as prognosis markers in oral squamous cell carcinoma(John Wiley and Sons, 2018-05) Peterle, Gabriela Tonini; Maia, Lucas Lima; Trivilin, Leonardo Oliveira; Oliveira, Mayara Mota de; Santos, Joaquim Gasparini dos; Mendes, Suzanny Oliveira; Stur, Elaine; Agostini, Lidiane Pignaton; Rocha, Lília Alves; Moysés, Raquel Ajub; Cury, Patrícia Maluf; Nunes, Fábio Daumas; Louro, Iúri Drumond; Santos, Marcelo dos; Silva, Adriana Madeira Alvares daBackground: In oral squamous cell carcinoma (OSCC), the HIF-1 complex promotes the expression of genes involved in specific mechanisms of cell survival under hypoxic conditions, such as plasminogen activator inhibitor-1 (PAI-1), carbonic anhydrase 9 (CAIX), and vascular endothelial growth factor A (VEGFA). The study aimed to investigate the presence and prognostic value of PAI-1, CAIX, and VEGFA in OSCC. Materials and Methods: Immunohistochemistry was used to analyze the expressions of these proteins in 52 tumoral tissue samples of patients with OSCC, surgically treated and followed by a minimum of 24 months after surgery. The correlations between protein expressions and clinicopathological parameters and prognosis were analyzed. Results: Positive PAI-1 membrane expression was significantly associated with local disease relapse (P = .027). Multivariate analysis revealed that the positive PAI-1 membrane expression is an independent marker for local disease relapse, with approximately 14-fold increased risk when compared to negative expression (OR = 14.49; CI = 1.40-150.01, P = .025). Strong PAI-1 cytoplasmic expression was significantly associated with the less differentiation grade (P = .027). Strong CAIX membrane expression was significantly associated with local disease-free survival (P = .038). Positive CAIX cytoplasmic expression was significantly associated with lymph node affected (P = .025) and with disease-specific survival (P = .022). Multivariate analysis revealed that the positive CAIX cytoplasmic expression is an independent risk factor for disease-related death, increasing their risk approximately 3-fold when compared to negative expression (HR = 2.84; CI = 1.02-7.87, P = .045). Positive VEGFA cytoplasmic expression was significantly associated with less differentiation grade (P = .035). Conclusion: Our results suggest a potential role for these expressions profiles as tumor prognostic markers in OSCC patients