Navegando por Autor "Santana, Sergio Carvalho de"
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Artigo Enhancing purification of chitosanase from Metarhizium anisopliae by expanded bed adsorption chromatography using Doehlert design(Elsevier, 2015-10-04) Santos, Everaldo Silvino dos; Santana, Sergio Carvalho de; Filho, Raimundo Cosme da Silva; Oliveira, Jackson Araújo de; Macedo, Gorete Ribeiro de; Padilha, Francine FerreiraThe use of expanded bed adsorption with three different adsorbents Streamline (SP XL, DEAE and PHENYL) to purify the chitosanase from Metarhizium anisopliae strain with and without clarification using Doehlert design for the optimization of the experiments has been investigated. Chitosanases are enzymes that hydrolyze chitosan, a carbohydrate, resulting oligosaccharides that have many biological remarkable activities, such as anti-cancer, anti-HIV and anti-oxidants activities. The superficial velocity was evaluated in five levels, from 40 to 260 cm h1, while the type of adsorbent, a qualitative variable, was assessed at three levels, saving materials and time according to the Doehlert design. The results showed that it is possible to purify chitosanase from M. anisopliae by expanded bed adsorption. Chit-osanase purification factor ranged from 2 to 3.1-fold and yield ranged from 27.7 to 45.3%. The highest enzyme activity in the elution was 0.06 U/mL values which were higher than or close to those reported. The molecular mass of chitosanase was about 45 kDa using SDS-PAGEArtigo Modeling and simulation of breakthrough curves during purification of two chitosanases from Metarhizium anisopliae using ion-exchange with expanded bed adsorption chromatography(Springer Nature Switzerland, 2014-02-17) Santos, Everaldo Silvino dos; Santana, Sergio Carvalho de; Silva Filho, Raimundo Cosme da; Cavalcanti, Jorge dos Santos; Oliveira, Jackson Araujo de; Macedo, Gorete Ribeiro de; Padilha, Francine FerreiraA mathematical model was developed to predict breakthrough curves during purification of the two chitosan- ases from Metarhizium anisopliae by expanded bed adsorption, taking into account the axial dispersion of liquid and using Streamline DEAE and SP XL adsorbents, anion and cation exchange resins, respectively. All the experiments were performed without clarification (with cells) aiming at the reduction of unit operations in future projects of separation processes, thereby reducing capital and operating costs. Chitosanases are enzymes that hydrolyze the carbohydrate chitosan, resulting in oligosaccharides that have many remarkable biological activities, such as anti-cancer, anti-HIV and antioxidant activities. The two adsorbents had similar performance in relation to hydrodynamics and mass transfer. The results of the parametric sensitivity analysis agree with the literature, and the model was validated with an average high degree of fit (94.68%) between simulated and experimental data obtained in this work