Navegando por Autor "Roças, Isabela N."
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Artigo Bacteriologic investigation of the effects of sodium hypochlorite and chlorhexidine during the endodontic treatment of teeth with apical periodontitis(2007) Siqueira Junior, José F.; Roças, Isabela N.; Paiva, Simone S. M.; Guimarães-Pinto, Tatiana; Magalhães, Karen M.; Lima, Kenio Costa deObjective This clinical study was undertaken to compare the effectiveness of 2.5% sodium hypochlorite (NaOCl) and 0.12% chlorhexidine digluconate as irrigants in reducing the cultivable bacterial populations in infected root canals of teeth with apical periodontitis. Study design According to stringent inclusion/exclusion criteria, 32 teeth with primary intraradicular infections and chronic apical periodontitis were selected and followed in the study. Bacterial samples were taken at the baseline (S1) and after chemomechanical preparation using either NaOCl (n = 16) or chlorhexidine (n = 16) as irrigants (S2). Cultivable bacteria recovered from infected root canals at the 2 stages were counted. Isolates from S2 samples were identified by means of 16S rRNA gene sequencing analysis. Results At S1, all canals were positive for bacteria, and the median number of bacteria per canal was 7.32 × 105 for the NaOCl group and 8.5 × 105 for the chlorhexidine group. At S2, the median number of bacteria in canals irrigated with NaOCl and chlorhexidine was 2.35 × 103 and 2 × 102, respectively. Six of 16 (37.5%) canals from the NaOCl group and 8 of 16 (50%) canals from the chlorhexidine group yielded negative cultures. Chemomechanical preparation using either solution substantially reduced the number of cultivable bacteria in the canals. No significant difference was observed between the NaOCl and chlorhexidine groups with regard to the number of cases yielding negative cultures (P = .72) or quantitative bacterial reduction (P = .609). The groups irrigated with NaOCl or chlorhexidine showed a mean number of 1.3 and 1.9 cultivable species per canal, respectively. The great majority of isolates in S2 were from gram-positive bacteria, with streptococci as the most prevalent taxa. Conclusions The present findings revealed no significant difference when comparing the antibacterial effects of 2.5% NaOCl and 0.12% chlorhexidine used as irrigants during the treatment of infected canals.Artigo Efficacy of instrumentation techniques and irrigation regimens in reducing the bacterial population within root canals(2002) Siqueira Junior, José F.; Roças, Isabela N.; Santos, Sandra R. L. D.; Lima, Kenio Costa de; Magalhães, Fernando A. C.; Uzeda, Milton deThe purpose of this study was to compare the in vitro intracanal bacterial reduction produced by using two instrumentation techniques and different irrigation methods. Root canals inoculated with Enterococcus faecalis were prepared by using the following techniques and irrigants: alternated rotary motions (ARM) technique, hand nickel-titanium files and 2.5% sodium hypochlorite (NaOCl) as irrigant; ARM technique and combined irrigation with 2.5% NaOCl and citric acid; ARM technique and combined irrigation with 2.5% NaOCl and 2% chlorhexidine gluconate; and Greater Taper rotary files, using 2.5% NaOCl as irrigant. Controls were instrumented by using the ARM technique and irrigated with sterile saline. Canals were sampled before and after preparation. After serial dilution, samples were plated onto Mitis-Salivarius agar, and the colony forming units that were grown were counted. All test techniques and solutions significantly reduced the number of bacterial cells within the root canal (p < 0.05). There was no significant difference between the experimental groups (p > 0.05). Nonetheless, all of them were significantly more effective than the control group (p < 0.05). These findings support the importance of using antimicrobial irrigants during the chemomechanical preparation, regardless of the solutions or instrumentation techniques used.Artigo Relationship between FcY receptor and interleukin-1 gene polymorphisms and post-treatment apical periodontitis(2009) Siqueira Junior, José F.; Roças, Isabela N.; Provenzano, José C.; Daibert, Frederico K.; Silva, Marlei G.; Lima, Kenio Costa deIntroduction Genetic polymorphisms have been reported to act as modifiers of diverse diseases and, as such, might theoretically influence the severity and response to treatment of apical periodontitis. The purpose of this study was to investigate the association of Fcγ receptor and interleukin (IL)-1 gene polymorphisms with post-treatment apical periodontitis in Brazilian individuals. Methods The study population consisted of 18 patients with post-treatment apical periodontitis and 44 individuals with root canal–treated teeth exhibiting healthy/healing periradicular tissues (controls). Patients were typed for the following genes (alleles): FcγRIIA (R131 or H131), FcγRIIIB (NA1 or NA2), IL-1A (1 or 2), and IL-1B (1 or 2). Results No significant statistical differences were observed for all specific genotypes and almost all allele carriage rates of the test genes as well as combinations thereof with regard to association with disease (P > .05). Actually, only 2 genetic conditions were found to be associated with post-treatment apical periodontitis: carriage of allele H131 of the FcγRIIa gene (P = .04) and a combination of this allele with allele NA2 of the FcγRIIIb gene (P < .01). Conclusions Data from the present study suggest that some conditions associated with polymorphism of Fcγ receptor genes might influence the patient's response to endodontic treatment of teeth with apical periodontitis.