Navegando por Autor "Oliveira, Denise Hélen Imaculada Pereira de"
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Artigo Correlation between cell cycle proteins and hMSH2 in actinic cheilitis and lip cancer(2016) Lopes, Maria Luiza Diniz de Sousa; Oliveira, Denise Hélen Imaculada Pereira de; Sarmento, Dmitry José de Santana; Queiroz, Lelia Maria Guedes; Miguel, Marcia Cristina da Costa; Silveira, Ericka Janine Dantas daThis study aims to evaluate and verify the relationship between the immunoexpression of hMSH2, p53 and p21 in actinic cheilitis (AC) and lower lip squamous cell carcinoma (SCC) cases. Forty AC and 40 SCC cases were submitted to immunoperoxidase method and quantitatively analyzed. Expression was compared by Mann–Whitney test, Student t test or one-way ANOVA. To correlate the variables, Pearson’s correlation coefficient was calculated. The expression of p53 and p21 showed no significant differences between histopathological grades of AC or lower lip SCC (p > 0.05). Immunoexpression of p53 was higher in SCC than in AC (p < 0.001), while p21 expression was more observed in AC when compared to SCC group (p = 0.006). The AC group revealed an inverse correlation between p53 and hMSH2 expression (r = −0.30, p = 0.006). Alterations in p53 and p21 expression suggest that these proteins are involved in lower lip carcinogenesis. Moreover, p53 and hMSH2 seem to be interrelated in early events of this process.Artigo Distribution of Hypoxia-Inducible Factor-1α and Glucose Transporter-1 in Human Tongue Cancers(2015) Vasconcelos, Marcelo Gadelha; Vasconcelos, Rodrigo Gadelha; Oliveira, Denise Hélen Imaculada Pereira de; Santos, Edilmar de Moura; Pinto, Leão Pereira; Silveira, Ericka Janine Dantas da; Queiroz, Lelia Maria GuedesPURPOSE: Oral squamous cell carcinomas have the potential for rapid and unlimited growth. Therefore, hypoxic tissue areas are common in these malignant tumors and contribute to cancer progression, therapy resistance, and poor outcomes. The aim of the present study was to analyze the gene product distribution of hypoxia-inducible factor-1α (HIF-1α) and glucose transporter-1 (GLUT-1) in cases of tongue squamous cell carcinoma (TSCC) and to identify a preliminary correlation between these proteins and clinical staging and Brynes's histologic grading system (HGS). MATERIALS AND METHODS: The sample included 57 cases of TSCC. Histologic sections of 3 μm were submitted to the immunoperoxidase method and semiquantitative analysis. The association between HIF-1α and GLUT-1 expression in TSCC and the clinical stage and the HGS of Bryne (1998) was evaluated using the χ(2) test, with the significance level set at 0.05 (α = 0.05). RESULTS: HIF-1α was mainly expressed in the nucleus/cytoplasm of neoplastic cells, most specimens exhibited diffuse staining in neoplastic cells (84.2%), and focal staining was only observed in perinecrotic areas (15.8%). GLUT-1 was expressed in the cytoplasm and membrane of malignant cells, and diffuse staining was observed in all cases. The intensity of HIF-1α expression correlated significantly with clinical stage (P = .011) and HGS (P = .002). A significant association was observed between the distribution of HIF-1α expression and metastasis (P = .040). Immunoexpression of GLUT-1 correlated significantly with clinical stage (P = .002) and HGS (P = .000). GLUT-1 expression in the peripheral island was predominant in most low-grade tumors (78.6%); in the high-grade cases, the expression prevailed in the location center/periphery (55.8%). Comparison of the location of the tumor island in the different histologic grades showed a statistically significant difference (P = .025). CONCLUSION: The expression of HIF and GLUT proteins within TSCC appears to be associated with disease stage, grade, and the presence of metastases. Additional studies are needed to evaluate the diagnostic and prognostic uses of these proteins in the treatment of TSCC.Dissertação Expressão Imuno-histoquímica das proteínas GLUT-1 e HIF-1α em lesões vasculares de mucosa oral(Universidade Federal do Rio Grande do Norte, 2012-02-16) Oliveira, Denise Hélen Imaculada Pereira de; Queiroz, Lélia Maria Guedes; ; http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4793681A0&dataRevisao=null; ; http://lattes.cnpq.br/9274393714692170; Alves, Pollianna Muniz; ; http://lattes.cnpq.br/4860117599607892; Silveira, Ericka Janine Dantas da; ; http://lattes.cnpq.br/2186658404241838O correto diagnóstico histológico de lesões vasculares em mucosa oral é fundamental, sobretudo na hora de definir o tratamento e prognóstico, visto que algumas dessas lesões apresentam involução. Este trabalho analisou a expressão imunohistoquímica da proteína humana transportadora de glicose (GLUT-1), em tumores vasculares benignos orais e reclassificou tais lesões de acordo com sua imunoexpressão. Além disso, avaliou a expressão imuno-histoquímica do fator 1 induzível por hipóxia (HIF-1α), principal fator de transcrição envolvido na adaptação celular à hipóxia. Foram analisados 60 casos de tumores vasculares benignos orais, sendo 30 casos com diagnóstico histológico de HEM e 30 casos de granulomas piogênicos orais (GP). Os resultados desta pesquisa demonstraram que das 30 lesões inicialmente classificadas como HEM, apenas 7 apresentaram imuno-positividade para GLUT-1, permanecendo com o diagnóstico inicial. As 23 restantes foram reclassificadas em malformação vascular (MV) 13 casos e GP 10 casos . Todos os casos da amostra com diagnóstico inicial de GP apresentaram-se negativos para GLUT-1. Quanto à imunoexpressão do HIF-1α, o teste de Mann-Whitney revelou diferença estatisticamente significativa entre os casos de GP e MV(p=0,002), onde a mediana do GP (m=78) foi superior a da MV (m=53). Com base nesses resultados, este estudo mostrou que o diagnóstico histológico por si só nem sempre é suficiente para o diagnóstico correto do HEM oral e que o HIF- 1α participa da patogênese das lesões vascularesArtigo Intraoral molluscum contagiosum in a young immunocompetent patient(2012) Carvalho, Cyntia Helena Pereira de; Andrade, Ana Luiza Dias Leite de; Oliveira, Denise Hélen Imaculada Pereira de; Lima, Emeline das Neves de Araújo; Silveira, Ericka Janine Dantas da; Medeiros, Ana Miryam Costa deMolluscum contagiosum (MC) is a contagious disease caused by a virus of the poxvirus family. In children, the disease commonly manifests as a variable number of discrete umbilicated papules on the face and trunk. In healthy and immunosuppressed adults, the disease appears on or near the genital organs and is often sexually transmitted. MC involving the intraoral mucosa has been documented but is rare. We report a case of MC involving the oral mucosa exclusively and discuss the main clinical, histopathologic, and therapeutic characteristics, comparing the findings with cases of this rare oral presentation described in the literatureArtigo Large solitary fibrous tumor of the oral cavity - Report of a case(2014) Oliveira, Denise Hélen Imaculada Pereira de; Albuquerque, Assis Filipe Medeiros; Barreto, Matheus Dantas de Araújo; Nonaka, Cassiano Francisco Weege; Silva, José Sandro Pereira da; Germano, Adriano Rocha; Queiroz, Lélia Maria GuedesThe solitary fibrous tumor (SFT) is a rare soft tissue tumor with a substantially benign clinical behavior. The SFT of the oral cavity is a very uncommon entity. It is also of complicated diagnosis because of its extensive morphologic diversity and because of its similarity to many mesenchymal tumors. A 44-year-old man was referred for management of an asymptomatic lesion in the left buccal mucosa, which had been identified 10 years earlier. Intra-oral examination revealed a well-demarcated, fibroelastic, rounded exophytic mass located in the left buccal mucosa. The mass was covered with a non-ulcerated mucosa of normal color and measured approximately 4.0 cm in diameter. Histopathological examination showed proliferation of spindle-shaped cells arranged in fascicles and in a patternless pattern, highly vascularized, with focal staghorn vessels. Immunohistochemical analysis revealed diffuse positivity for CD34 and focal positivity for Bcl-2. Awareness of the morphological diversity of SFT coupled to a judicious use of appropriate immunohistochemical probes should prove valuable to accurately segregate SFT from other spindle cell neoplasms.