Navegando por Autor "Oliveira, Camila Aparecida Machado de"
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Artigo Insulin modulates myogenesis and muscle atrophy resulting from skin scald burn in young male rats(Elsevier, 2020-08-17) Quintana, Hananiah Tardivo; Baptista, Vivianne Izabelle de Araújo; Lazzarin, Mariana Cruz; Antunes, Hanna Karen Moreira; Le Sueur-Maluf, Luciana; Oliveira, Camila Aparecida Machado de; Oliveira, Flavia deBurn injuries (BIs) due to scalding are one of the most common accidents among children. BIs greater than 40% of total body surface area are considered extensive and result in local and systemic response. We sought to assess morphological and myogenic mechanisms through both short- and long-term intensive insulin therapies that affect the skeletal muscle after extensive skin BI in young rats. Materials and methods: Wistar rats aged 21 d were distributed into four groups: control (C), control with insulin (C þ I), scald burn injury (SI), and SI with insulin (SI þ I). The SI groups were submitted to a 45% total body surface area burn, and the C þ I and SI þ I groups received insulin (5 UI/Kg/d) for 4 or 14 d. Glucose tolerance and the homeostatic model assessment of insulin resistance index were determined. Gastrocnemius muscles were analyzed for histopathological, morphometric, and immunohistochemical myogenic parameters (Pax7, MyoD, and MyoG); in addition, the expression of genes related to muscle atrophy (MuRF1 and MAFbx) and its regulation (IGF-1) were also assessed. Results: Short-term treatment with insulin favored muscle regeneration by primary myogenesis and decreased muscle atrophy in animals with BIs, whereas the long-term treatment modulated myogenesis by increasing the MyoD protein. Both treatments improved histopathological parameters and secondary myogenesis by increasing the MyoG protein. Conclusions: Treatment with insulin benefits myogenic parameters during regeneration and modulates MuRF1, an important mediator of muscle atrophy