Navegando por Autor "Nunes, F. D."
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Artigo DAP1 high expression increases risk of lymph node metastases in squamous cell carcinoma of the oral cavity(Fundação de Pesquisas de Ribeirão Preto, 2015-09-08) Santos, Marcelo dos; Maia, L. L.; Silva, C. V. M.; Peterle, G. T.; Mercante, A. M. C.; Nunes, F. D.; Carvalho, M. B.; Tajara, E. H.; Louro, I. D.; Silva-Conforti, A. M. A.Death-associated protein 1 (DAP1) is a member of the DAP family. Its expression is associated with cell growth and normal death of the neoplastic cells, regulated by the mammalian target of the rapamycin protein. Activated DAP1 negatively regulates autophagy, which has been associated with the development and progression of several diseases, such as cancer, and with prognosis and survival of diverse tumor types. Therefore, in this study we analyzed DAP1 expression in 54 oral squamous cell carcinoma tumor samples and in 20 non-tumoral margins by immunohistochemistry. The results showed that DAP1 is more frequently expressed in tumor tissues compared with marginal non-tumoral cells. Additionally, high DAP1 expression is associated with a 4-fold increase in the risk of lymph node metastases. Our results suggest that the DAP1 protein can be used as a potential marker of lymph node metastases predisposition, helping define the best therapy for each patient to minimize risk of developing metastasesArtigo FAS ligand expression in inflammatory infiltrate lymphoid cells as a prognostic marker in oral squamous cell carcinoma(Fundação de Pesquisas de Ribeirão Preto, 2015-09-22) Peterle, G. T.; Santos, Marcelo dos; Mendes, S. O.; Carvalho-Neto, P. B.; Maia, L. L.; Stur, E.; Agostini, Lidiane Pignaton; Silva, C. V. M.; Trivilin, L. O.; Nunes, F. D.; Carvalho, M. B.; Tajara, E. H.; Louro, I. D.; Silva-Conforti, A. M. A.Currently, the most important prognostic factor in oral squamous cell carcinoma (OSCC) is the presence of regional lymph node metastases, which correlates with a 50% reduction in life expectancy. We have previously observed that expression of hypoxia genes in the tumor inflammatory infiltrate is statistically related to prognosis in OSCC. FAS and FASL expression levels in OSCC have previously been related to patient survival. The present study analyzed the relationship between FASL expression in the inflammatory infiltrate lymphoid cells and clinical variables, tumor histology, and prognosis of OSCC. Strong FASL expression was significantly associated with lymph node metastases (P = 0.035) and disease-specific death (P = 0.014), but multivariate analysis did not confirm FASL expression as an independent death risk factor (OR = 2.78, 95%CI = 0.81-9.55). Diseasefree and disease-specific survival were significantly correlated with FASL expression (P = 0.016 and P = 0.005, respectively). Multivariate analysis revealed that strong FASL expression is an independent marker for earlier disease relapse and disease-specific death, with approximately 2.5-fold increased risk compared with weak expression (HR = 2.24, 95%CI = 1.08-4.65 and HR = 2.49, 95%CI = 1.04-5.99, respectively). Our results suggest a potential role for this expression profile as a tumor prognostic marker in OSCC patientsArtigo Leptin receptor expression and Gln223Arg polymorphism as prognostic markers in oral and oropharyngeal cancer(Fundação de Pesquisas de Ribeirão Preto, 2015-11-24) Rodrigues, P. R. S.; Maia, L. L.; Santos, Marcelo dos; Peterle, G. T.; Alves, L. U.; Takamori, J. T.; Souza, R. P.; Barbosa, W. M.; Mercante, A. M. C.; Nunes, F. D.; Carvalho, M. B.; Tajara, E. H.; Louro, I. D.; Silva-Conforti, A. M. A.The leptin gene product is released into the blood stream, passes through the blood-brain barrier, and finds the leptin receptor (LEPR) in the central nervous system. This hormone regulates food intake, hematopoiesis, inflammation, immunity, differentiation, and cell proliferation. The LEPR Gln223Arg polymorphism has been reported to alter receptor function and expression, both of which have been related with prognostics in several tumor types. Furthermore, several studies have shown a relationship between the Gln223Arg polymorphism and tumor development, and its role in oral and oropharyngeal squamous cell carcinoma is now well understood. In this study, 315 DNA samples were used for LEPR Gln223Arg genotyping and 87 primary oral and oropharyngeal squamous cell carcinomas were used for immunohistochemical expression analysis, such that a relationship between these and tumor development and prognosis could be established. Homozygous LEPR Arg223 was found to be associated with a 2-fold reduction in oral and oropharyngeal cancer risk. In contrast, the presence of the Arg223 allele in tumors was associated with worse disease-free and disease-specific survival. Low LEPR expression was found to be an independent risk factor, increasing the risk for lymph node metastasis 4-fold. In conclusion, the Gln223Arg polymorphism and LEPR expression might be valuable markers for oral and oropharyngeal cancer, suggesting that LEPR might serve as a potential target for future therapies