Navegando por Autor "Moura, Geovanna Maria Medeiros"

Agora exibindo 1 - 2 de 2

Evaluation of heterologous protein expression at different concentrations of MGSO4 and IPTG in Escherichia Coli W110
(Atena Editora, 2019) Santos, Yago Queiroz dos; Carelli, Gabriella Silva Campos; Veras, Bruno Oliveira de; Cruz, Joelton Igor Oliveira da; Moura, Geovanna Maria Medeiros; Marques Neto, Antônio Moreira; França, Anderson Felipe Jácome de
Since the improvement of gene sequencing techniques, the expression of heterologous proteins is gaining increasing relevance given the possibility of large-scale production when compared to the isolation of these same molecules in their original sources. However, heterologous expression systems continue to require for a suitable induction relevant amounts of synthetic molecules like IPTG (Isopropyl β-D-1-thiogalactopyranoside) which have toxicity and cost limitation when used in large scale. Thus, the objective of this work was to evaluate the effects of bivalent salts such as MgSO4 in association with IPTG on heterologous expression system pDMI.1 for a possible better cost-benefit proposal in the current heterologous protein expression protocol. Magnesium Sulfate contributes to an increase in bacterial physiology as a whole, inducing a higher protein expression acting as an indirect and alternative inducer in these conditions, with the advantages of presenting low cost and low cellular toxicity. Based on these obtained data, we propose the usage of low MgSO4 molar concentrations in current heterologous protein expression protocols performed in our group for the pDMI.1 vector. Additional studies are still needed in order to evaluate the effects of this salt on different expression systems at wide range of microorganisms
Phylogenetic analysis and identification of a cellulase producing Bacillus Sp. Strain by 16s RRNA sequencing
(Atena Editora, 2019) Santos, Yago Queiroz dos; França, Anderson Felipe Jácome de; Veras, Bruno Oliveira de; Carelli, Gabriella Silva Campos; Moura, Geovanna Maria Medeiros; Cruz, Joelton Igor Oliveira da; Oliveira, Fernanda Granja da Silva; Oliveira, João Ricardhis Saturnino de; Amorim, Luciclaudio Cassimiro de; Santos, Elizeu Antunes dos
The microorganisms belonging to genus Bacillus include a wide spectra and ubiquitous group of bacteria that can be found from the forest soil, to marine ecosystems occurring in association with a variety of aquatic organisms such as scleractinian corals present in the intertidal boulders. Therefore, these microorganisms are exposed to various abiotic stresses that cause ecological selection for a physiologically adapted microbiota to such extremes of temperature and salinity. In this work, we isolated and characterized a cellulase from a marine bacterial strain and indentified its specie by 16S rRNA sequencing followed by a BLAST analysis. The cellulolytic strain called SR22 showed to be a gram-positive spore-forming bacilli, facultative anaerobe, and catalase positive, as well as negative for indole, H2S production, and citrate utilization; those findings led us to consider the isolate belonging to the genus Bacillus, which was confirmed by the phylogenetic analysis, which revealed that the SR22 strain formed a clade with Bacillus subtilis. Its nucleotide sequence was deposited in GenBank as Accession No. MH119099 and the degree of sequence similarity of strain SR22 to Bacillus sp. was 99%. Taken together, the present data indicate the present celulase-positive indentified strain as a potential and useful candidate for industrial applications that employs celulase degrading processes like second-generation bioethanol and paper industries being still necessary further studies to complete characterize this microorganism secretome

SIGAA

Navegando por Autor "Moura, Geovanna Maria Medeiros"