Navegando por Autor "Morais, Ana Heloneida de Araujo"
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Artigo Anti-inflammatory agents as modulators of the inflammation in adipose tissue: a systematic review(Plos One, 2022) Morais, Ana Heloneida de Araujo; Nascimento, Sara Sayonara da Cruz; Queiroz, Jaluza Luana Carvalho de; Medeiros, Amanda Fernandes de; Nunes, Ana Clara de França; Piuvezam, Grasiela; Maciel, Bruna Leal Lima; Passos, Thais Souza; https://orcid.org/0000-0002-6460-911XObesity is characterized by an adipose tissue mass expansion that presents a risk to health, associated with a chronic increase in circulating inflammatory mediators. Anti-inflammatory agents are an obesity alternative treatment. However, the lack of effective agents indicates the need to assess the mechanisms and identify effective therapeutic targets. The present work identified and described the mechanisms of action of anti-inflammatory agents in adi pose tissue in experimental studies. The review was registered in the International Prospec tive Registry of Systematic Reviews (PROSPERO—CRD42020182897). The articles’ selection was according to eligibility criteria (PICOS). The research was performed in PubMed, ScienceDirect, Scopus, Web of Science, VHL, and EMBASE. The methodological quality evaluation was assessed using SYRCLE. Initially, 1511 articles were selected, and at the end of the assessment, 41 were eligible. Among the anti-inflammatory agent classes, eight drugs, 28 natural, and five synthetic compounds were identified. Many of these anti inflammatory agents act in metabolic pathways that culminate in the inflammatory cytokines expression reduction, decreasing the macrophages infiltration in white and adipose tissue and promoting the polarization process of type M1 to M2 macrophages. Thus, the article cla rifies and systematizes these anti-inflammatory agents’ mechanisms in adipose tissue, pre senting targets relevant to future research on these pathways.Artigo Antibacterial action mechanisms and mode of trypsin inhibitors: a systematic review(Taylor & Francis, 2022) Morais, Ana Heloneida de Araujo; Nascimento, Amanda Maria de Souza; Oliveira Segundo, Victor Hugo de; Aguiar, Ana Julia Felipe Camelo; Piuvezam, Grasiela; Passos, Tha ıs Souza; Damasceno, Karla Suzanne Florentino da Silva Chaves; http://lattes.cnpq.br/1233944493334651This systematic review (SR) aimed to gather studies describing the antibacterial action mechanisms and mode of trypsin inhibitors. The review protocol was registered (PROSPERO: CRD42020189069). Original articles resulting from studies in animal models, in bacterial culture, and using cells that describe antibac terial action of trypsin inhibitor-type peptides or proteins were selected in PubMed, Science Direct, Scopus, Web of Science, BVS, and EMBASE. The methodological quality assessment was performed using the PRISMA and OHAT tool. 2382 articles were retrieved, 17 of which were eligible. Four studies demon strated the action mechanism directly on the bacterial membrane, and the fifth study on endogenous pro teases extracted from the bacteria themselves. The antibacterial action mode was presented in the other studies, which can generate bacteriostatic or bactericidal effects without describing the mechanisms. This study generated information to enable new preclinical or clinical studies with molecules contributing to public health.Artigo Antibacterial action mechanisms of trypsin inhibitors: a protocol for systematic review and meta-analysis(Medicine, 2021) Morais, Ana Heloneida de Araujo; Nascimento, Amanda Maria de Souza; Matias, Lídia Leonize Rodrigues; Oliveira Segundo, Victor Hugo de; Piuvezam, Grasiela; Passos, Thaís Souza; Damasceno, Karla Suzanne Florentino da Silva Chaves; https://orcid.org/0000-0002-6460-911XIntroduction: Infectious diseases caused by bacteria represent one of the challenges in human healthcare, mostly caused by resistant bacteria, increasing the treatment cost, and fatal health complications. Researchers worldwide seek new therapeutic strategies to combat these highly resistant bacteria. Trypsin inhibitor peptides or proteins have innumerous bioactivities, such as antibacterial activity, which makes them potential candidates to treat diseases caused by bacteria. Thus, this study protocol describes a systematic review concerning the action mechanisms related to these molecules’ antibacterial activity. Methods: This systematic review protocol was elaborated according to the guidelines outlined in the Preferred Reporting Items for Systematic Reviews and Meta-Analysis Protocols (PRISMA-P). The databases PubMed, ScienceDirect, Scopus, Web of Science, and Virtual Health Library will be used. Experimental studies carried out with rats and/or mice of both sexes, without water or diet restriction and in vitro (bacterial culture) studies and in cell, treated with trypsin inhibitor-type peptides or proteins that have a possible antibacterial action will be included. If at least two studies present clinical and/or methodological and/or statistical homogeneity, a meta-analysis will be carried out at the end of the analysis. The selection of studies, data extraction, and methodological quality assessment will be performed independently by two reviewers. Results: This protocol will be the basis for a systematic review. It is expected to identify several manuscripts highlighting the mechanisms related to the action of trypsin inhibitor peptides or proteins on bacteria. Conclusion: The systematic review based on this protocol will gather knowledge about trypsin inhibitor peptides or proteins’ antibacterial action mechanisms. It will provide subsidies for new research involving these molecules’ application against infectious diseases caused by bacteria. Ethics and dissemination: The present work does not involve any humans or animals; therefore, ethical approval is not needed. Prospero Registration Number: This review was registered with the International Register of Prospective Systematic Reviews on Jun 11, 2020 (registration: CRD42020189069). Available at: https://www.crd.york.ac.uk/prospero/display_record.php? RecordID=189069.Artigo Beneficial effects of tamarind trypsin inhibitor in chitosan–whey protein nanoparticles on hepatic injury induced high glycemic index diet: a preclinical study(International Journal Molecular Sciences, 2021) Morais, Ana Heloneida de Araujo; Aguiar, Ana J. F. C.; Queiroz, Jaluza L. C. de; Santos, Pedro P. A.; Camillo, Christina S.; Serquiz, Alexandre C.; Costa, Izael S.; Oliveira, Gerciane S.; Gomes, Ana F. T.; Matias, Lídia L. R.; Costa, Rafael O. A.; Passos, Thaís S.; https://orcid.org/0000-0002-6460-911XSeveral studies have sought new therapies for obesity and liver diseases. This study investigated the effect of the trypsin inhibitor isolated from tamarind seeds (TTI), nanoencapsulated in chitosan and whey protein isolate (ECW), on the liver health status of the Wistar rats fed with a high glycemic index (HGLI) diet. The nanoformulations without TTI (CW) and ECW were obtained by nanoprecipitation technique, physically and chemically characterized, and then administered to the animals. The adult male Wistar rats (n = 20) were allocated to four groups: HGLI diet + water; standard diet + water; HGLI diet + ECW (12.5 mg/kg); and HGLI diet + CW (10.0 mg/kg), 1 mL per gagave, for ten days. They were evaluated using biochemical and hematological parameters, Fibrosis 4 Index for Liver Fibrosis (FIB-4), AST to Platelet Ratio Index (APRI) scores, and liver morphology. Both nanoparticles presented spherical shape, smooth surface, and nanometric size [120.7 nm (ECW) and 136.4 nm (CW)]. In animals, ECW reduced (p < 0.05) blood glucose (17%), glutamic oxalacetic transaminase (39%), and alkaline phosphatase (24%). Besides, ECW reduced (p < 0.05) APRI and FIB-4 scores and presented a better aspect of hepatic morphology. ECW promoted benefits over a liver injury caused by the HGLI dietArtigo Can polyphenols improve the gut health status in pre-clinical study with diet-induced obesity? a protocol for systematic review and/or meta-analysis(Medicine, 2021) Morais, Ana Heloneida de Araujo; Dias, Lêda Karla Monteiro; Medeiros, Gidyenne Christine Bandeira Silva de; Silva, Ana Karolinne Nascimento; Maia, Juliana Kelly da Silva; https://orcid.org/0000-0002-6460-911XIntroduction: Obesity is characterized as a low-grade inflammation that impairs physiological functions, including intestinal functioning and gut microbiota balance. Dietary polyphenols can be a strategy for obesity management, collaborating to preserve or recover gut health through antioxidant and anti-inflammatory actions, as well as modulators of the microbiota. This study describes a systematic review protocol to elucidate effects of polyphenols on intestinal health of pre-clinical models with diet induced obesity. AIM: Our aim is to evaluate evidence about polyphenols’ effects in the gut microbiota composition and diversity, parameters of the physical and molecular status of the gut barrier in obese models, additionally, understand the possible involved mechanisms. Methodology: A protocol was developed and published on PROSPERO (Registration No: CRD42021262445). Preferred Reporting Items for Systematic Review and Meta-Analysis Protocols is used to outline the protocol. The articles will be selected according to the PICOS strategy (population, interventions, control, outcome, and study design) in the following databases: PubMed, Science Direct, Scopus, Web of Science, and EMBASE. Experimental studies performed on rats and mice with a control group that describes treatment with polyphenols (from food matrix or crude extracts or isolated compounds) at any frequency, time, and dose will be included. Two reviewers will, independently, select the papers, extract data, and evaluate the data quality. The Systematic Review Center for Laboratory Animal Experimentation (SYRCLE) tool will be used to assess the risk of bias. Expected results:Results will be showed through of native synthesis and, if possible, a metanalysis will be conducted. The review produced with this protocol can show the scientific evidence level about polyphenols’ effects in intestinal health in obesity status. Abbreviations: CFU = colony-forming unit, MeSH = Medical Subject Headings, OTUs = Operational taxonomic units, PICOS = population, interventions, control, outcomes, and study design, PRISMA = Preferred Reporting Items for Systematic Reviews and Meta-Analyses, PRISMA-P = Preferred Reporting Items for Systematic Reviews and Meta-Analyses Protocols, PROSPERO = International Prospective Register of Systematic Reviews, SFCA = Short-chain fatty acids, SYRCLE = Systematic Review Center for Laboratory Animal ExperimentationTese Caracterização estrutural e avaliação da atividade biológica de uma nova hipotensina identificada no veneno do escorpião Tityus stigmurus(2016-04-28) Machado, Richele Janaina Araujo; Pedrosa, Matheus de Freitas Fernandes; ; ; Morais, Ana Heloneida de Araujo; ; Dore, Celina Maria Pinto Guerra; ; Dantas, Deyse de Souza; ; Barbosa, Euzebio Guimaraes;A identificação, caracterização estrutural e avaliação da atividade biológica de peptídeos bioativos em veneno de animais peçonhentos tem sido alvo de investigação científica. O escorpião Tityus stigmurus pertence à família Buthidae e predomina no Nordeste do Brasil. O repertório molecular da glândula do veneno do escorpião T. stigmurus foi investigado pelo nosso grupo de pesquisa e permitiu a identificação de uma variedade de peptídeos de interesse biotecnológico incluindo peptídeos aniônicos, antimicrobianos (AMPs), toxinas atuantes em canais de sódio e potássio, peptídeos hipotéticos secretados, metaloproteases, peptídeos ricos em cisteína e peptídeos potenciadores de bradicinina (BPPs). Uma variedade de atividades importantes já foram descritas para alguns destes peptídeos, tais como atividade antibacteriana, antifúngica, anticancerígeno e imunomoduladora. Diversos componentes do veneno atuam sobre o sistema cardiovascular, como os BPPs que são peptídeos com propriedade hipotensora e fortes candidatos para o desenho de novos fármacos para o tratamento de doenças cardiovasculares. O presente trabalho apresentou como objetivo realizar a caracterização estrutural e avaliação da atividade biológica de uma nova hipotensina identificada no veneno do escorpião T. stigmurus. O cluster TSTI0006C, obtido do transcriptoma de glândulas de veneno, foi analisado e sua estrutura primária foi reduzida após a determinação do peptídeo sinal e pró-peptídeo, resultando em uma estrutura de 25 resíduos de aminoácidos, denominado de TistH (T. stigmurus Hypotensin), com massa molecular de 2,7 kDa, ausência de cisteína e presença de dois resíduos de prolina na região C-terminal, característica estrutural típica dos BPPs. O alinhamento múltiplo de TistH com outros BPPs resultou em um percentual de 76% de identidade com os BPPs identificados no veneno do escorpião T. serrulatus. Análises realizadas in silicoe por dicroísmo circular revelaram que TistH apresenta estrutura tridimensional predominante em α-hélice. A estrutura de TistH foi estável em função a variação de pH e temperatura. Em ensaios de atividade biológica, TistH não apresentou ação hemolítica em sangue de cavalo, não alterou a viabilidade de células normais e cancerígenas, assim como não estimulou a liberação de NO em meio de cultura de células Raw e a migração de leucócitos em modelo de bolsa de ar em camundongos Swiss. TistH apresentou atividade com MIC de 128 µg/mL contra as cepas C. albicans (LM-106), C. tropicalis (ATCC 13308) e A. flavus (LM-247 e LM-26). Além disso, foi avaliado o efeito cardiovascular de TistH em ratos normotensos. TistH foi capaz de potencializar a ação hipotensora de bradicinina e induzir um efeito vaso-relaxante em anéis da artéria mesentérica com endotélio dependente de óxido nítrico e independente da enzima conversora de angiotensina (ECA). Os dados obtidos nesse estudo podem contribuir para a elucidação das características estruturais e funcionais de TistH, uma molécula multifuncional capaz de reduzir a pressão arterial de ratos e inibir o crescimento de fungos, apresentando baixa citotoxicidade, sendo portanto um peptídeo bioativo candidato a utilização como agente farmacológico.Artigo Effect of carotenoid encapsulation on antioxidant activities: a protocol for systematic review(Medicine, 2020) Morais, Ana Heloneida de Araujo; Queiroz, Jaluza Luana Carvalho de; Medeiros, Isaiane; Piuvezam, Grasiela; Nunes, Ana Clara de França; Gomes, Camila Carvalho; Maciel, Bruna Leal Lima; Passos, Thaís Souza; https://orcid.org/0000-0002-6460-911XBackground: Carotenoids play essential roles in human health, such as antioxidant activity, and therefore can decrease free radicals oxidation action, preventing numerous diseases. However, these compounds have an unstable nature, turning them susceptible to adverse conditions in food processing and storage. Thereby the search for alternatives that maintain and enhance carotenoid antioxidant function, such as encapsulation, has grown. The objective of this study was to establish a systematic review protocol to evaluate the effect of different encapsulation techniques on the antioxidant action of carotenoids, evaluating which one is the best and safest, and their role in enhancing the antioxidant activity. Methods: This protocol was guided by the preferred reporting items for protocols for systematic reviews and meta-analyzes. The databases to be searched are PubMed, EMBASE, Scopus, ScienceDirect, and Web of Science. Experimental studies conducted in rats and mice (in vivo) of both sexes and ages, evaluating the use of encapsulated and crude carotenoids will be included in the systematic review. The characteristics of the studies, the experimental model, and the main results will be described, and the risk of bias assessment will be evaluated. Three independent reviewers will proceed with the selection of studies, data extraction, and methodological quality assessment. A narrative synthesis will be made for the included studies. Besides, if sufficient qualitative data is available, a meta-analysis will be conducted. I2 statistics will be used to assess heterogeneity. Results: This protocol will guide the production of a systematic review that can determine the effect of different encapsulation techniques and encapsulating agents on the antioxidant action of carotenoids. Thus, it will enable the determination of the best encapsulation techniques to promote the preservation and increase of the antioxidant activity, contributing to future research that may reproduce the best carotenoid encapsulation technique in an animal model. Conclusion: The systematic review to be produced from this protocol will provide support for the construction of research that evaluates the effect of encapsulation on the antioxidant function of carotenoids and its possible application as a nutraceutical, considering that this functionality is directly associated with health promotion. Record of systematic review: This review was recorded in the International Register of Prospective Systematic Reviews on January 22, 2020 (registration: CRD42020142065). Available at: https://www.crd.york.ac.uk/prospe /display_record.php?ID=CRD42020142065 Abbreviations: CAPES = coordination of improvement of higher education personnel, CNPQ = National Council for Scientific and Technological Development, MeSH = Medical Subject HeadingsDissertação Identificação da segurança e potencial aplicação clínica de nanopartículas contendo inibidor de tripsina isolado de sementes de tamarindo (Tamarindus indica L.)(2019-03-29) Costa, Rafael Oliveira de Araújo; Morais, Ana Heloneida de Araujo; Uchoa, Adriana Ferreira; ; ; ; Serquiz, Alexandre Coelho; ; Rocha, Hugo Alexandre de Oliveira;Moléculas naturais com ação bioativa, tais como o inibidor de tripsina isolado de sementes de tamarindo (Tamarindus indica L.) (ITT), destaca-se entre os agentes sacietogênicos e anti-inflamatórios estudados na atualidade. Nessa perspectiva, visando manter o ITT intacto e protegido ao longo do trato gastrointestinal, foi realizada a encapsulação por nanoprecipitação em quitosana purificada e proteína de soro de leite isolada, e as partículas (EQPI) foram caracterizadas por diferentes técnicas. Para isso, porém, precisa ser avaliada a segurança, visando possíveis aplicações clínicas. Dessa forma, o objetivo deste estudo foi avaliar a citoxicidade e toxicidade sanguínea subaguda da dose bioativa do EQPI em ratos Wistar, alimentados com dieta de alto índice glicêmico. O ITT foi obtido com base no fracionamento proteico, utilizando sulfato de amônio. A fração com maior atividade inibitória contra tripsina, fracionamento de 30-60%, foi isolada por Cromatografia de Afinidade em Tripsina-Sepharose, e utilizada para síntese de nanopartículas. O EQPI foi sintetizado, respeitando a proporção de ITT, quitosana purificada e proteína isolada do soro do leite de 1:2:2 p/p/p, respectivamente, sendo posteriormente caracterizado por diferentes análises físicas e químicas. E avaliado quanto à interação entre o ITT e os agentes encapsulantes em pH neutro (água) e em pH ácido, por meio de diversas etapas de filtração em Amicon® 100 K, sendo monitorado pela atividade antitríptica. Adicionalmente, foi avaliado a citotoxicidade do EQPI em diferentes concentrações (0,5, 2,5 e 5 mg/mL) por meio do ensaio de resazurina, utilizando duas linhagens celulares, (Caco-2 e CCD-18Co), além da toxicidade sanguínea (hemograma, função hepática e renal) em ratos Wistar macho (n = 10), alimentados com dieta de alto índice glicêmico e, administrado com EQPI (12,5 mg/Kg) por 10 dias. O EQPI apresentou partículas esféricas com superfície lisa de acordo com a Microscopia Eletrônica de Varredura, diâmetro médio de 118 nm (17.27), índice de polidispersão de 0.373 (0.02), carga superficial de -38.26 mV (0.15) e, eficiência de encapsulação de 95.3% (0.31). Quanto à interação em água do ITT e dos agentes encapsulantes por meio da avaliação da atividade antitríptica, utilizando Amicon® 100 K, observou-se que, o EQPI protegeu o ITT e o manteve aprisionado. No entanto, em pH ácido ocorreu uma liberação gradual do ITT, sendo evidenciada pela forte interação entre o ITT e os agentes encapsulantes utilizados neste estudo. O ensaio de citotoxicidade apontou alta viabilidade celular (>70%) para Caco-2 e CCD-18Co, quando exposta ao EQPI avaliado em diferentes concentrações. Quanto à toxicidade sanguínea subaguda do EQPI, administrado em ratos Wistar, também não foram demonstrados efeitos tóxicos de acordo com os resultados obtidos para os parâmetros bioquímicos avaliados. Neste estudo, o EQPI se mostrou eficiente em proteger o ITT quando disperso em água e exposto ao pH ácido. As concentrações de EQPI, avaliadas em células e no modelo experimental, não demonstraram sinais de toxicidade, sugerindo a aplicação segura dessas partículas e provável efeito hepatoprotetor. Assim, assegurando a sua aplicação em estudos complementares para investigar a eficácia no tratamento da obesidade e suas complicações.Artigo Mechanism of the action of bioactive proteins of vegetables in diabetes mellitus type 2: systematic review protocol(Medicine, 2019) Morais, Ana Heloneida de Araujo; Medeiros, Amanda Fernandes de; Costa, Izael de Sousa; Piuvezam, Grasiela; Medeiros, Gidyenne Christine Bandeira Silva de; Maciel, Bruna Leal Lima; https://orcid.org/0000-0002-6460-911XBackground: Diabetes mellitus type 2 (DM2) is a chronic disease of significant prevalence causing hyperglycemia and several comorbidities. Evidences highlight the performance of non - protein bioactive compounds found in vegetables in the control of hyperglycemia. This study describes a protocol of a systematic review, which analyzes the action of proteins and bioactive peptides of plants in DM2. Methods: The Preferred Reporting Items guide this protocol for Systematic Reviews and Meta-Analyzes Protocols (PRISMA-P) was used. The databases that will be used for searching will be PubMed, ScienceDirect, Scopus, Web of Science, EMBASE, and Virtual Health Library, Brazil (VHL). Studies that use bioactive proteins and peptides of vegetal origin in DM2 will be included in the systematic review. The studies will be identified using clinical parameters and the effect on insulin resistance. The characteristics of the studies as control groups, test substance, dosage, intervention time, and the main results will be described. Selection of studies, data extraction, and methodological quality assessment will be performed independently by two experienced reviewers. Results: This protocol will be the basis for a systematic review identifying the mechanism of action of plant proteins and peptides in type 2 diabetes mellitus. Conclusion: Systematic reviews from this protocol will provide support for the construction of researches that analyze the effect of plant bioactive proteins and peptides on the control of hyperglycemia and how these molecules act in the control of DM2Artigo Mechanisms of action of molecules with anti-TNF alpha activity on intestinal barrier inflammation: a systematic review protocol(Medicine, 2019) Morais, Ana Heloneida de Araujo; Lima, Mayara Santa Rosa; Lima, Vanessa Cristina Oliveira de; Piuvezam, Grasiela; Azevedo, Kesley Pablo Morais de; Maciel, Bruna Leal Lima; https://orcid.org/0000-0002-6460-911XBackground: Tumor necrosis factor-alpha (TNF-alpha), among cytokines that mediate the inflammatory process, plays an important role in diseases involving the loss of intestinal barrier integrity. Several molecules with anti-TNF-alpha activity have been studied aiming to develop new therapies. The purpose of this paper is to describe the systematic review protocol of experimental studies that determine mechanisms of action of molecules with anti-TNF-alpha activity on intestinal barrier inflammation. Methods:This protocol is guided by the Preferred Reporting Items for Systematic Reviews and Meta-Analyzes Protocols (PRISMA P). The databases to be searched are PubMed, EMBASE, Scopus, ScienceDirect, and Web of Science. Experimental studies in rats or mice that assessed the activity of anti-TNF-alpha molecules in models of intestinal barrier inflammation will be included in the systematic review. Studies characteristics, experimental model, and main results will be described and the bias risk assessment will be performed. Two independent reviewers will perform study selection, data extraction, and methodological quality assessment. A narrative synthesis will be made for the included studies. Also, if sufficient data is available, a meta-analysis will be conducted.I2 statistics will be used to assess heterogeneity. Results: The present protocol will assist in producing a systematic review that identifies the mechanisms underlying the reduction of TNF-alpha in intestinal barrier inflammation models. Conclusion: The systematic review may contribute to the theoretical basis of research on new molecules with anti-TNF-alpha potential and, consequently, in the development of new therapies employed in humans.Artigo What are the digestion and absorption models used to reproduce gastrointestinal protein processes? a protocol for systematic review(Medicine, 2021) Morais, Ana Heloneida de Araujo; Luz, Anna Beatriz Santana; Costa, Rafael Oliveira de Araujo; Medeiros, Gidyenne Christine Bandeira Silva de; Piuvezam, Grasiela; Passos, Thais Souza; https://orcid.org/0000-0002-6460-911XBackground: Animal, cell, and in vitro studies have been applied to simulate the human gastrointestinal tract (GIT) and evaluate the behavior of biomolecules. Understanding the peptides and/or proteins stability when exposed to these physiological conditions of the GIT can assist in the application of these molecules in the treatment of diseases such as obesity. This study describes a protocol of systematic reviews to analyze the methodologies that mimic the digestive and absorptive processes of peptides and/or proteins. Methods: The protocol follows the guidelines described by Preferred Reporting Items for Systematic Reviews and Meta-Analyzes Protocols (PRISMA-P). The search strategies will be applied in the electronic databases PubMed, ScienceDirect, Scopus, Web of Science, Evidence portal, Virtual Health Library, and EMBASE. The intervention group will be formed by in vivo, in cells, and in vitro (gastrointestinal simulating fluids) studies of digestion and absorption of peptides and/or proteins presenting a schedule, duration, frequency, dosages administered, concentration, and temperature, and the control group consisting in studies without peptides and/ or proteins. The selection of studies, data extraction, and assessment of the risk of bias will be carried out independently by 2 reviewers. For animal studies, the risk of bias will be assessed by the instrument of the Systematic Review Center for Experimentation with Laboratory Animals (SYRCLE) and the Office of Health Assessment and Translation (OHAT) tool will be used to assess the risk of bias in cell studies. Results: This protocol contemplates the development of 2 systematic reviews and will assist the scientific community in identifying methods related to the digestive and absorptive processes of peptides and/or proteins. Conclusion: Both systematic reviews resulting from this protocol will provide subsidies for the construction of research related to the clinical application of bioactive peptides and/or proteins. In this context, they will make it possible to understand the gastrointestinal processes during administering these molecules, as the gastrointestinal environment can affect its functionality. Therefore, validating the effectiveness of these protocols is important, as it mimics in vitro biological conditions, reducing the use of animals, being consistent with the reduction, refine and replace programArtigo What are the mechanisms of action of anti-inflammatory agents in adipose tissue? a protocol for systematic review and meta-analysis(Medicine, 2021) Morais, Ana Heloneida de Araujo; Nascimento, Sara Sayonara da Cruz; Queiroz, Jaluza Luana Carvalho de; Medeiros, Amanda Fernandes de; Nunes, Ana Clara de França; Piuvezam, Grasiela; Maciel, Bruna Leal Lima; Passos, Thaís Souza; https://orcid.org/0000-0002-6460-911XBackground: Obesity is a disease characterized by the abnormal accumulation of adipose tissue in the body, triggering a chronic subclinical state of inflammation. Bioactive compounds, given their anti-inflammatory properties, are a safe and promising alternative in controlling the inflammatory condition of obesity. This study describes a systematic review protocol aiming to analyze the anti inflammatory molecules mechanisms and compounds action on adipocytes. Methods: Preferred Reporting Items for Systematic Review and Meta-Analysis Protocols (PRISMA-P) will outline the protocol and PRISMA to the systematic review. The databases used for research will be PubMed, Science Direct, Scopus, Web of Science, BVS, and EMBASE. Experimental studies performed on rats and mice with a control group that describes treatment with anti-inflammatory agents (drugs, nutraceuticals, bio active compounds, among others) at any frequency, time, and dose will be included. Three independent reviewers will select studies and extract data. The evaluation of the methodological quality of each research will be performed using the SYRCLE tool. If at least 2 studies show clinical and/or methodological and/or statistical homogeneity, a meta analysis will be performed, using the RevMan Analyzes statistical package in Review Manager v.5.3. Results: In this study, we hope to find a considerable number of articles presenting mechanisms involved in the action of anti inflammatory molecules and compounds on adipocytes. Conclusion: The systematic review produced from this protocol will present evidence on the mechanisms involved in the action of anti-inflammatory molecules and compounds in adipocytes. It will also contribute to developing new research and new insights about anti-inflammatory therapies with a future application view. Record of systematic review: This review was registered with the International Register of Prospective Systematic Reviews on May 18, 2020 (registration: CRD42020182897). Available at: https://www.crd.york.ac.uk/prospero/display_record.php?ID= CRD42020182897. Abbreviations: 5-LOX = enzyme 5-lipoxygenase, AMPK = AMP-activated protein kinase, BAT = brown adipose tissue, CAPES = Coordination of Improvement of Higher Education Personnel, CNPq = National Council for Scientific and Technological Development, COX-2 = enzyme cyclooxygenase, IL-6 = Interleukin-6, iNOS = nitric oxide-synthase induced, NF-kB = factor kappa B, Nrf2 = nuclear factor erythroid 2-related factor 2, PICOS = population, interventions, control, and study results, PPAR-g = peroxisome proliferator-activated receptor gamma, PRISMA = Preferred Reporting Items for Systematic Reviews and Meta Analyses, PRISMA-P = Preferred Reporting Items for Systematic Reviews and Meta-Analyses Protocols, PROSPERO = International Prospective Register of Systematic Reviews, SREBP-1c = sterol regulatory element-binding protein 1, Syrcle = Systematic Review Center for Laboratory Animal Experimentation, TLR = Toll-like receptors, TNF-a = Tumor Necrosis Factor alpha, WAT = white adipose tissue, WHO = World Health Organization.