Navegando por Autor "Lima, Mayara S. R."
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Artigo Anti-Inflammatory protein isolated from tamarind promotes better histological aspects in the intestine regardless of the improvement of intestinal permeability in a preclinical study of diet-induced obesity(Nutrients, 2022) Morais, Ana Heloneida de Araújo; Lima, Mayara S. R.; Gonçalves, Catarina; D. Neto, Mafalda; Macedo, Maria Helena; Queiroz, Jaluza L. C. de; Silva, Valéria C. da; Costa, Izael de S.; Camillo, Christina da S.; Santos, Pedro Paulo de A.; Lima, Aldo A. M.; Pastrana, Lorenzo; Maciel, Bruna L. L.; https://orcid.org/0000-0002-6460-911XObesity is associated with metabolic and physiological effects in the gut. In this study, we evaluated the anti-inflammatory effect of trypsin inhibitor isolated from tamarind seeds (TTI) in vitro (interaction with lipopolysaccharide (LPS) and inhibitory activity against human neutrophil elastase (HNE)), and using intestinal co-cultures of Caco-2:HT29-MTX cell lines inflamed with TNF α (50 ng/mL) and a Wistar rat model of diet-induced obesity (n = 15). TTI was administered to animals by gavage (10 days), and the treated group (25 mg/kg/day) was compared to animals without treatment or treated with a nutritionally adequate diet. In the in vitro study, it showed inhibitory activity against HNE (93%). In co-cultures, there was no protection or recovery of the integrity of inflamed cell monolayers treated with TTI (1.0 mg/mL). In animals, TTI led to lower plasma concentrations of TNF-α and IL-6, total leukocytes, fasting glucose, and LDL-c (p < 0.05). The intestines demonstrated a lower degree of chronic enteritis, greater preservation of the submucosa, and greater intestinal wall thickness than the other groups (p = 0.042). Therefore, the better appearance of the intestine not reflected in the intestinal permeability added to the in vitro activity against HNE point to possibilities for new studies and applications related to this activity.Artigo Breast milk retinol levels after Vitamin A supplementation at different postpartum amounts and intervals(Nutrients, 2022) Ribeiro, Karla Danielly da S.; Bezerra, Danielle S.; Melo, Andressa T. A. de; Oliveira, Kátia C. de A. N. de; Araújo, Karoline Q. M. A. de; Medeiros, Monalisa S. M. de F.; Santos, Flávia A. P. S. dos; Medeiros, Jeane F. P.; Lima, Mayara S. R.; Silva, Ana Gabriella C. L. da; Dimenstein, Roberto; Osório, Mônica M.; 0000-0002-2251-5967Maternal vitamin A (VA) supplementation in risk areas for Vitamin A deficiency (VAD) was launched to improve the level of this nutrient in nursing mothers and in their breast milk. This longitudinal and randomized study aimed to evaluate the levels of retinol in breast milk after supplementation with VA in varying amounts (200,000 IU or 400,000 IU) and different postpartum intervals. Women were distributed into four intervention groups and given a single 200,000 IU postnatal dosage of VA at time 0 h (postnatal morning) (G200 0H); a single 200,000 IU dosage of VA in week four (G200 4W); 200,000 IU of VA at time 0 h + 200,000 IU of VA 24 h after the first supplementation (G400 24H); and 200,000 IU of VA at time 0 h + 200,000 IU of VA one week after the first supplementation (G400 1W). Breast milk samples were collected over a 12-week period (0 h, 24 h and 1, 4, 12 weeks post-natal). Retinol levels were determined by high-performance liquid chromatography. The Generalized Estimated Equation (GEE) assessed the different retinol levels. The G200 (0H), G400 (24H), and G400 (1W) groups presented higher retinol levels at 24 h than the G200 (4W) group (p < 0.001). The retinol levels of all groups were similar at times 1, 4 and 12 weeks after delivery (p > 0.05). Maternal VA supplementation increased retinol levels in the colostrum. Different supplementation dosages or postpartum administration times did not result in added benefit to retinol levels in mature breast milk.Artigo Nanoparticles containing tamarind isolate protein potentiate the satiety without promoting the anti-Inflammatory effect in a preclinical model of diet-Induced obesity(Foods, 2022) Morais, Ana Heloneida de Araújo; Costa, Rafael O. A.; Medeiros, Isaiane; Queiroz, Jaluza L. C. de; Matias, Lídia L. R.; Lima, Mayara S. R.; Oliveira, Gerciane S. de; Aguiar, Ana Júlia F. C.; Costa, Izael S.; Silva, Eloyse Mikaelly de S.; Santos, Nicolle Caroline S. dos; Passos, Thaís S.; https://orcid.org/0000-0002-6460-911XThe study aimed to evaluate the nanoparticles (ECW) containing tamarind trypsin inhibitor (TTI) concerning the storage effect under different conditions on antitrypsin activity and the bioactive potential in a preclinical model. ECW was exposed to different pH and temperatures to evaluate the interaction between TTI and its encapsulating agents, monitored by antitrypsin activity. Wistar rats (n = 25) with obesity induced by diet were divided into groups: untreated; treatment with nutritionally adequate diet; treatment with nutritionally adequate diet and ECW/12.5 mg/kg; treatment with ECW/12.5 mg/kg; and treatment with TTI/25 mg/kg. The groups were evaluated over ten days with regards to satiety, zoometric, biochemical, and inflammatory parameters, using ten times less TTI (2.5 mg/kg) contained in ECW. TTI was protected and encapsulated in ECW without showing residual inhibitory activity. Only at gastric pH did ECW show antitrypsin activity. At different temperatures, it showed high antitrypsin activity, similar to TTI. The animals treated with ECW had significantly reduced body weight variation (p < 0.05), and only TTI treatment reduced the inflammatory parameters significantly (p < 0.05). The study showed that by using lower concentrations of TTI in ECW it was possible to perceive promising effects with perspectives of use in functional products for managing obesity and its complications