Navegando por Autor "Lima, Maíra Conceição Jerônimo de Souza"
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Artigo An insulin receptor-binding multifunctional protein from tamarindus indica L. presents a hypoglycemic effect in a diet-induced type 2 diabetes-preclinical study(Foods, 2022-07) Maciel, Bruna Leal Lima; Costa, Izael de Sousa; Lima, Mayara Santos Rosa; Medeiros, Amanda; Bezerra, Lucas Lima; Santos, Paula; Serquiz, Alexandre; Lima, Maíra Conceição Jerônimo de Souza; Oliveira, Gerciane Santos de; Santos, Elizeu Antunes dos; Monteiro, Norberto de Kássio Vieira; Morais, Ana HeloneidaThe objectives of this study were to evaluate the hypoglycemic effect of the trypsin inhibitor isolated from tamarind seeds (TTI) in an experimental model of T2DM and the in silico interaction between the conformational models of TTI 56/287 and the insulin receptor (IR). After inducing T2DM, 15 male Wistar rats were randomly allocated in three groups (n = 5): 1—T2DM group without treatment; 2—T2DM group treated with adequate diet; and 3—T2DM treated with TTI (25 mg/kg), for 10 days. Insulinemia and fasting glucose were analyzed, and the HOMA-IR and HOMA-β were calculated. The group of animals treated with TTI presented both lower fasting glucose concentrations (p = 0.0031) and lower HOMA-IR indexes (p = 0.0432), along with higher HOMA-β indexes (p = 0.0052), than the animals in the other groups. The in silico analyses showed that there was an interaction between TTIp 56/287 and IR with interaction potential energy (IPE) of −1591.54 kJ mol−1 (±234.90), being lower than that presented by insulin and IR: −894.98 kJ mol−1 (±32.16). In addition, the presence of amino acids, type of binding and place of interaction other than insulin were identified. This study revealed the hypoglycemic effect of a bioactive molecule of protein origin from Tamarind seeds in a preclinical model of T2DM. Furthermore, the in silico analysis allowed the prediction of its binding in the IR, raising a new perspective for explaining TTI’s action on the glycemic responseArtigo Safety and bioactive potential of nanoparticles containing Cantaloupe melon (Cucumis melo L.) carotenoids in an experimental model of chronic inflammation(Elsevier, 2020) Morais, Ana Heloneida de Araújo; Medeiros, Isaiane; Oliveira, Grazielle Louise Ribeiro de; Queiroz, Jaluza Luana Carvalho de; Gomes, Camila de Carvalho; Carvalho, Fabiana Maria Coimbra de; Lima, Maíra Conceição Jerônimo de Souza; Serquiz, Alexandre Coelho; Santos, Pedro Paulo de Andrade; Camillo, Christina da Silva; Maciel, Bruna Leal Lima; Passos, Thaís Souza; https://orcid.org/0000-0002-6460-911XThe safety and bioactive potential of crude carotenoid extract from Cantaloupe melon nanoencapsulated in porcine gelatin (EPG) were evaluated in a chronic inflammatory experimental model. Animals were fed a high glycemic index and high glycemic load (HGLI) diet for 17 weeks and treated for ten days with 1) HGLI diet, 2) standard diet, 3) HGLI diet + crude carotenoid extract (CE) (12.5 mg/kg), and 4) HGLI diet + EPG (50 mg/kg). General toxicity signals were investigated, considering body weight, food intake, hematological, biochemical parameters, relative weight, morphology, and histopathology of organs. The biochemical parameters indicated the low toxicity of EPG. Acute hepatitis was observed in animals' livers, but CE and EPG groups presented improved tissue appearance. Chronic enteritis was observed in animals, with villi and intestinal glands preservation in the EPG group. The results suggest the safety and the bioactive effect of EPG, possibly related to its anti-inflammatory potentialArtigo A trypsin Inhibitor from tamarind reduces food intake and improves inflammatory status in rats with metabolic syndrome regardless of weight loss(Nutrients, 2016-09) Maciel, Bruna Leal Lima; Carvalho, Fabiana Maria Coimbra de; Lima, Vanessa Cristina Oliveira de; Costa, Izael de Souza; Medeiros, Amanda Fernandes de; Serquiz, Alexandre Coelho; Lima, Maíra Conceição Jerônimo de Souza; Serquiz, Raphael Paschoal; Uchôa, Adriana Ferreira; Santos, Elizeu Antunes dos; Morais, Ana Heloneida de AraújoTrypsin inhibitors are studied in a variety of models for their anti-obesity and anti-inflammatory bioactive properties. Our group has previously demonstrated the satietogenic effect of tamarind seed trypsin inhibitors (TTI) in eutrophic mouse models and anti-inflammatory effects of other trypsin inhibitors. In this study, we evaluated TTI effect upon satiety, biochemical and inflammatory parameters in an experimental model of metabolic syndrome (MetS). Three groups of n = 5 male Wistar rats with obesity-based MetS received for 10 days one of the following: (1) Cafeteria diet; (2) Cafeteria diet + TTI (25 mg/kg); and (3) Standard diet. TTI reduced food intake in animals with MetS. Nevertheless, weight gain was not different between studied groups. Dyslipidemia parameters were not different with the use of TTI, only the group receiving standard diet showed lower very low density lipoprotein (VLDL) and triglycerides (TG) (Kruskal–Wallis, p < 0.05). Interleukin-6 (IL-6) production did not differ between groups. Interestingly, tumor necrosis factor-alpha (TNF-α) was lower in animals receiving TTI. Our results corroborate the satietogenic effect of TTI in a MetS model. Furthermore, we showed that TTI added to a cafeteria diet may decrease inflammation regardless of weight loss. This puts TTI as a candidate for studies to test its effectiveness as an adjuvant in MetS treatment