Navegando por Autor "Ferreira, Diana Aline Nôga Morais"
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Artigo Diazepam causes sedative rather than anxiolytic effects in C57BL/6J mice(Springer Science and Business Media LLC, 2021-04-29) Reis, Marina Pádua; Ferreira, Diana Aline Nôga Morais; Tort, Adriano Bretanha Lopes; Blunder, MartinaDiazepam has been broadly accepted as an anxiolytic drug and is often used as a positive control in behavioral experiments with mice. However, as opposed to this general assumption, the effect of diazepam on mouse behavior can be considered rather controversial from an evidence point of view. Here we revisit this issue by studying the effect of diazepam on a benchmark task in the preclinical anxiety literature: the elevated plus maze. We evaluated the minute-by-minute time-course of the diazepam effect along the 10 min of the task at three different doses (0.5, 1 and 2 mg/kg i.p. 30 min before the task) in female and male C57BL/6J mice. Furthermore, we contrasted the effects of diazepam with those of a selective serotoninergic reuptake inhibitor (paroxetine, 10 mg/kg i.p. 1 h before the task). Diazepam had no anxiolytic effect at any of the tested doses, and, at the highest dose, it impaired locomotor activity, likely due to sedation. Noteworthy, our results held true when examining male and female mice separately, when only examining the first 5 min of the task, and when animals were subjected to one hour of restrain-induced stress prior to diazepam treatment. In contrast, paroxetine significantly reduced anxiety-like behavior without inducing sedative effects. Our results therefore suggest that preclinical studies for screening new anxiolytic drugs should be cautious with diazepam use as a potential positive controlDissertação Efeito anticonvulsivante de frações isoladas da peçonha da formiga Dinoponera quadriceps (Formicidae: Ponerinae)(Universidade Federal do Rio Grande do Norte, 2015-05-11) Ferreira, Diana Aline Nôga Morais; Ribeiro, Alessandra Mussi; ; http://lattes.cnpq.br/7373640456805525; ; http://lattes.cnpq.br/3077781936933336; Silva, Regina Helena da; ; http://lattes.cnpq.br/0101190051087933; Santos, Wagner Ferreira dos; ; http://lattes.cnpq.br/8806122525697090A epilepsia é uma patologia crônica do sistema nervoso central que afeta cerca de 65 milhões de indivíduos no mundo. Aproximadamente 30% desses indivíduos desenvolvem crises convulsivas que persistem apesar do tratamento monitorado com drogas antiepiléptica s. Assim, há uma evidente necessidade do desenvolvimento de novos fármacos antiepilépticos e as peçonhas podem ser uma excelente fonte de modelos. Nesse contexto, enquanto já vários estudos sobre peçonhas de serpentes, escorpiões e aranhas, pouco se sabe s obre as peçonhas de formigas. Estudos prévios do nosso laboratório demonstraram que a peçonha desnaturada da formiga Dinoponera quadríceps protegeu camundongos de crises convulsivas e morte induzidas por bicuculina (BIC). Nesse contexto, o objetivo desse t rabalho foi investigar o potencial anticonvulsivante de frações isoladas da peçonha de D. quadríceps em crises convulsivas induzidas pela BIC, bem como uma análise dos efeitos dessas frações no comportamento natural dos camundongos no campo aberto. Os anim ais foram divididos em grupos, os quais receberam injeções (1 mg/ml i.c.v.) de seis frações distintas e tiveram seu comportamento geral observado no campo aberto durante 3 0 min. No segundo experimento, o s animais receberam as mesmas frações 20 min antes da administração de bicuculina (10 mg/ml). Em seguida, foi analisado o comportamento motor convulsivo desses animais durante 30 minutos no campo aberto. No primeiro experimento, não foram observadas alterações comportamentais. Já no segundo experimento, a ad ministração prévia de DqTx1, DqTx3, DqTx4 e DqTx6 aumentou a latência para o desenvolvimento de crises tônico - clônicas. Além disso, todas as frações, exceto DqTx5, aumentaram a latência para a morte dos animais. Ainda, os melhores resultados foram obtidos com a fração DqTx6, que protegeu 62,5% dos animais testados contra o desenvolvimento de crises tônico - clônicas e 100% dos animais contra a morte.Artigo GluN2B and GluN2A containing NMDAR are differentially involved in extinction memory destabilization and restabilization during reconsolidation(Springer Science and Business Media LLC, 2021-01-08) Radiske, Andressa; Gonzalez, Maria Carolina; Ferreira, Diana Aline Nôga Morais; Rossato, Janine Inez; Bevilaqua, Lia Rejane Müller; Cammarota, Martín PabloExtinction memory destabilized by recall is restabilized through mTOR-dependent reconsolidation in the hippocampus, but the upstream pathways controlling these processes remain unknown. Hippocampal NMDARs drive local protein synthesis via mTOR signaling and may control active memory maintenance. We found that in adult male Wistar rats, intra dorsal-CA1 administration of the non-subunit selective NMDAR antagonist AP5 or of the GluN2A subunit-containing NMDAR antagonist TCN201 after step down inhibitory avoidance (SDIA) extinction memory recall impaired extinction memory retention and caused SDIA memory recovery. On the contrary, pre-recall administration of AP5 or of the GluN2B subunit-containing NMDAR antagonist RO25-6981 had no effect on extinction memory recall or retention per se but hindered the recovery of the avoidance response induced by post-recall intra-CA1 infusion of the mTOR inhibitor rapamycin. Our results indicate that GluN2B-containing NMDARs are necessary for extinction memory destabilization whereas GluN2A-containing NMDARs are involved in its restabilization, and suggest that pharmacological modulation of the relative activation state of these receptor subtypes around the moment of extinction memory recall may regulate the dominance of extinction memory over the original memory traceArtigo mTOR inhibition impairs extinction memory reconsolidation(Cold Spring Harbor Laboratory, 2020-12-15) Radiske, Andressa; Gonzalez, Maria Carolina; Ferreira, Diana Aline Nôga Morais; Rossato, Janine Inez; Bevilaqua, Lia Rejane Müller; Cammarota, Martín PabloFear-motivated avoidance extinction memory is prone to hippocampal brain-derived neurotrophic factor (BDNF)-dependent reconsolidation upon recall. Here, we show that extinction memory recall activates mammalian target of rapamycin (mTOR) in dorsal CA1, and that post-recall inhibition of this kinase hinders avoidance extinction memory persistence and recovers the learned aversive response. Importantly, coadministration of recombinant BDNF impedes the behavioral effect of hippocampal mTOR inhibition. Our results demonstrate that mTOR signaling is necessary for fear-motivated avoidance extinction memory reconsolidation and suggests that BDNF acts downstream mTOR in a protein synthesis-independent manner to maintain the reactivated extinction memory traceArtigo Pro-and anticonvulsant effects of the ant dinoponera quadriceps (Kempf) venom in mice(Springer, 2015) Ferreira, Diana Aline Nôga Morais; Cagni, Fernanda Carvalho; Santos, J. R.; Silva, D.; Azevedo, D. L. O.; Araújo, Arrilton; Silva, R. H.; Ribeiro, A. M.Epilepsy affects at least 50 million people worldwide, and the available treatment is associated with various side effects. Approximately 20–30% of the patients develop seizures that persist despite careful monitored treatment with antiepileptic drugs. Thus, there is a clear need for the development of new antiepileptic drugs, and the venoms can be an excellent source of probes. In this context, while there are studies on venoms from snakes, scorpions, and spiders, little is known regarding venom from ants. The aim of this study was to investigate the potential pro- and anticonvulsant effects of the venom from the ant Dinoponera quadriceps (Kempf) in Swiss mice. After the injection of the crude venom (DqTx—5, 50, and 500 mg/mL) in the lateral ventricle of mice, we observed a reduction of exploration and grooming behaviors, as well as an increase in immobility duration. In addition, the crude venom induced procursive behavior and tonicclonic seizures at the highest concentration. Conversely, the preadministration of the denatured venom (AbDq) at the concentration of 2 mg/mL protected the animals against tonic-clonic seizures (66.7%) and death (100%) induced by administration of bicuculline. Taken together, the findings demonstrate that D. quadriceps venom might be potential source of new pro- and anticonvulsants molecules