Navegando por Autor "Brandão-Neto, José"
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Artigo Antioxidant activity and protective effect of Turnera ulmifolia Linn. var. elegans against carbon tetrachloride-induced oxidative damage in rats(Elsevier, 2012-12) Brito, Naira J.N.; López, Jorge A.; Nascimento, Maria Aparecida do; Macêdo, José B.M.; Silva, Gabriel Araujo; Oliveira, Cláudia N.; Rezende, Adriana Augusto de; Brandão-Neto, José; Schwarz, Aline; Almeida, Maria das GraçasThe present study aimed to determine whether the leaves of Turnera ulmifolia Linn. var. elegans extract exert significant antioxidant activity. The antioxidant activity of its hydroethanolic extract (HEETU) was evaluated by assessing (a) its radical scavenging ability in vitro, and (b) its in vivo effect on lipid peroxidation and antioxidant enzyme activities. The in vitro antioxidant assay (DPPH) clearly supported HEETU free radical scavenging potential. Moreover, glutathione content and antioxidant enzyme activities (glutathione peroxidase, superoxide dismutase and catalase) were significantly enhanced in CCl4- treated rats due to oral HEETU-treatment (500 mg/kg b.w.) over 7 and 21 days. In addition, an improvement was observed in lipid peroxidation and serum biochemical parameters (aspartate aminotransferase and alanine aminotransferase), indicating a protective effect against CCl4-induced liver injuries, confirmed by histopathological studies. The HEETU effect was comparable to the standard drug Legalon (50 mg/kg b.w.) under the same experimental condition. Quantitative analysis of the HPLC extract revealed the presence of flavonoids, wich mediate the effects of antioxidant and oxidative stress. In conclusion, extract components exhibit antioxidant and hepatoprotective activities in vitro and in vivo.Artigo Citocinas inflamatórias na distrofia muscular de Duchenne: uma revisão narrativa(Revista Eletrônica Acervo Saúde, 2021-12) Vale, Sancha Helena de Lima; Marinho, Kívia Maria Batista; Cunha, Thais Alves; Grilo, Evellyn Câmara; Miranda, Carolinne Thaisa de Oliveira Fernandes; Brandão-Neto, José; Vermeulen-Serpa, Karina Marques; https://orcid.org/0000-0002-0972-1678; https://orcid.org/0000-0002-9932-2338; https://orcid.org/0000-0001-7647-6572; https://orcid.org/0000-0003-0021-5741Objetivo: Descrever achados na literatura sobre os marcadores inflamatórios na Distrofia Muscular de Duchenne (DMD) e entender qual é a sua associação com a progressão da doença. Revisão bibliográfica: A DMD é uma miopatia genética grave, caracterizada por um comprometimento ortopédico, cardíaco e respiratório e que conduz a uma morte precoce do indivíduo. A inflamação possui atribuição fundamental na fisiopatologia da DMD. Elevados níveis de citocinas e marcadores inflamatórios, como o IL-1β, TNF-α,IL-6 e IL-17, ativadores e recrutadores de células inflamatórias, parecem ter relação com a progressão da doença. Considerações finais: Os níveis plasmáticos, teciduais e musculares de marcadores inflamatórios são descritos como elevados em relação a indivíduos saudáveis. A associação entre os marcadores inflamatórios e a progressão da DMD parece ser uma particularidade da doença. Entender esta relação é importante para o estabelecimento de novas abordagens terapêuticas voltadas à DMD. No entanto, ainda há escassez sobre o assunto na literatura. Reforçando a necessidade de mais estudos abordando a temáticaArtigo Validity of bioelectrical impedance to estimate fat-free mass in boys with Duchenne muscular dystrophy(Plos One, 2020) Dourado Junior, Mário Emílio Teixeira; Grilo, Evellyn C.; Cunha, Thais A.; Costa, Ádila Danielly S.; Araújo, Bárbara G. M.; Lopes, Márcia Marília G. D.; Maciel, Bruna L. L.; Alves, Camila X.; Serpa, Karina M. Vermeulen; Lais, Lucia Leite; Brandão-Neto, José; Vale, Sancha Helena L.; https://orcid.org/0000-0002-9462-2294The evaluation of fat-free mass (FFM) in patients with Duchenne muscular dystrophy (DMD) is useful to investigate disease progression and therapeutic efficacy. This study aimed to validate the Bioelectrical impedance (BIA) method compared with the dual-energy X-ray absorptiometry (DXA) for estimating the %FFM in boys with DMD. This is a cross-sectional study performed with children and adolescents diagnosed with DMD. Resistance and reactance were measured with a BIA analyzer, from which eight predictive equations estimated the %FFM. The %FFM was also determined by DXA and its used as a reference method. Pearson correlation test, coefficient of determination, the root-mean-square error, the interclass correlation coefficient, and linear regression analysis were performed between %FFM values obtained by BIA and DXA. The agreement between these values was verified with the Bland-Altman plot analysis. Forty-six boys aged from 5 to 20 years were enrolled in the study. All the equations showed a correlation between the %FFM estimated by BIA and determined by DXA (p < 0.05). The Bland-Altman method indicated that two equations have a significant bias (p < 0.05) and six equations showed no significant bias of %FFM (p > 0.05). However, one of them has high variation and wide limits of agreement. Five of eight %FFM predictive equations tested in DMD were accurate when compared with the DXA. It can be concluded that BIA is a validity method to evaluate patients with DMD.Artigo When age matters: boys with Duchenne muscular dystrophy have growth delay and a fat mass accumulation, as they get older(Research, Society And Development, 2021) Dourado Junior, Mário Emílio Teixeira; Cunha, Thais Alves; Grilo, Evellyn Câmara; Costa, Ádila Danielly de Souza; Serpa, Karina Marques Vermeulen; Lais, Lúcia Leite; Brandão-Neto, José; Vale, Sancha Helena de Lima; https://orcid.org/0000-0002-9462-2294Anthropometry and body composition evaluation in boys with Duchenne muscular dystrophy (DMD) are challenging, but crucial methods to evaluate the nutritional status, and better anthropometric reference values and body composition predictive equations are needed for this population. Based on these aspects, this study aimed to investigate the hypothesis that changes in anthropometric parameters and body composition of boys with DMD occur according to age. A cross-sectional study with 49 individuals diagnosed with DMD at the neurological outpatient facility at the Onofre Lopes University Hospital in Natal, Brazil, was performed between September 2016 and March 2019. These individuals underwent anthropometric and body composition evaluation. According to age, the participants were divided into four groups: G1 (2.6 - 8.2y), G2 (8.5 - 10.8y), G3 (11.0 - 14.0y), and G4 (15.9 - 23.0y). The parameters weight-for-age (W/A) (p=0.025), tricipital skinfold (TSF) (p=0.027), adductor pollicis muscle (p=0.041), and corrected arm muscle area (cAMA) (p=0.005) were different among the groups. Regarding anthropometric parameters, was prevalence in the categories of appropriate W/A and a height-for-age (H/A), and eutrophy for body mass index-for-age (BMI/A). For the TSF, there was a higher frequency of severe malnutrition or obesity. The cAMA indicated severe malnutrition in most individuals. As for %FM, high adiposity was more frequent, increasing over age groups (G1 to G4). The boys with DMD presented different patterns of anthropometric and body composition parameters. An increase of fat mass and a decrease of lean mass with age/disease progression were observed.