Navegando por Autor "Azevedo, Ricardo Bentes de"
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Artigo Effects of photodynamic therapy mediated by nanoemulsion containing chloro-aluminum phthalocyanine: a histologic and immunohistochemical study in human gingiva(ELSEVIER, 2015) Moraes, Maiara de; Vasconcelos, Roseane Carvalho de; Longo, João Paulo Figueiró; Muehlmann, Luis Alexandre; Azevedo, Ricardo Bentes de; Lemos, Telma Maria Araújo Moura; Costa, Antonio de Lisboa LopesBackground Photodynamic therapy (PDT) uses photosensitizing agents, which are delivered in target cells, followed by local application of visible light in specific wavelengths. This reaction produce reactive oxygen species able to induce cell death by apoptosis or necrosis, injured to the local vasculature, and exert important effects on the immune system. Objective The present work evaluated the clinical findings, histomorphological alterations and immunodetection of VEGF after PDT using chloro-aluminum phthalocyanine (AlClPc) entrapped in a lipid nanoemulsion in a split-mouth clinical trial. Material and methods Eight healthy volunteers with clinical indication for extraction were included in the study. Seven days before the extraction 40 ul of nanoemulsion AlClPc 5 μM was injected into gingival tissue followed by irradiation with diode laser, the contralateral side was used as control. Tissue specimens were removed seven days after the PDT and divided into two groups (test and control groups) for histological and immunohistochemical analysis. Patients were monitored at days, 0, 7, 14 and 30 to assess adverse effects of the therapy. Results The therapy was well tolerated by all patients. Adverse effects were short-time and completely reversible. Areas of edema, vascular congestion, and intense vascularization were viewed in gingival samples that received PDT. Additionally, dystrophic calcification was observed in subepithelial region. VEGF showed moderate to strong immunostaining in specimens subjected to PDT. Conclusions Taken together, the results showed that the protocol used in this study mediated by nanoemulsion containing AlClPc is safe for clinical application in gingival tissue and suggests that VEGF is increased after PDT.Dissertação Nanopartículas catiônicas de poli (ácido lático) para liberação modificada de peptídios da peçonha do escorpião Tityus serrulatus(Universidade Federal do Rio Grande do Norte, 2015-07-24) Mesquita, Philippe de Castro; Silva Júnior, Arnobio Antonio da; Pedrosa, Matheus de Freitas Fernandes; ; http://lattes.cnpq.br/2929963416385218; ; http://lattes.cnpq.br/2593509584288129; ; http://lattes.cnpq.br/3801155631580819; Luchessi, André Ducati; ; http://lattes.cnpq.br/4420863418928278; Azevedo, Ricardo Bentes de; ; http://lattes.cnpq.br/9072332484355315Acidentes notificados envolvendo o envenenamento por escorpiões ainda são frequentes no Brasil, ocasionados principalmente pela espécie Tityus serrulatus, conhecido como escorpião amarelo. Embora os soros antiescorpiônicos sejam produzidos rotineiramente por diversos laboratórios oficiais, a eficácia de sua utilização depende da rapidez com que se inicia o tratamento e da eficiência na produção de anticorpos pelos animais imunizados. No presente trabalho, o desenvolvimento de nanopartículas poliméricas catiônicas de poli(ácido lático) teve como objetivo a busca de um sistema de liberação modificada para os peptídeos e proteínas presentes na peçonha do escorpião T. serrulatus, capaz de potencializar a produção de anticorpos em soro anti-veneno. As nanopartículas catiônicas foram obtidas por nanoprecipitação, após o estudo do efeito dos parâmetros da técnica sobre as propriedades físico-químicas das partículas, com a otimização de um método de baixa energia. A funcionalização da superfície das nanopartículas com a polietilenoimina hiper-ramificada foi comprovada pela análise de potencial zeta e possibilitou a adsorção por interação eletrostáticade diferentes tipos de proteínas. A eficiência de incorporação de 40-80 % de albumina de sorobovino (BSA) e 100 % de peptídeos da peçonha do escorpião avaliadas por espectrofotometria e eletroforese em gel de poliacrilamida, confirmou o sucesso na escolha dos parâmetros do método de obtenção das nanopartículas, produzidas com tamanho entre 100 a 250 nm. A análise de microscopia de força atômica e estudos in vitro de liberação, mostraram que as nanopartículas esféricas fornecem um perfil sustentado de liberação das proteínas pelo mecanismo de difusão, que potencializou a produção de anticorpos antiveneno em animais imunizados com as nanopartículas contendo a peçonha do escorpião, comprovando o potencial para aplicação in vivo das nanopartículas.Artigo Photodynamic therapy using chloro-aluminum phthalocyanine decreases inflammatory response in an experimental rat periodontal disease model(Elsevier, 2017-02) Araujo, Aurigena Antunes; Moraes, Maiara de; Vasconcelos, Roseane Carvalho; Longo, João Paulo Figueiró; Muehlmann, Luis Alexandre; Azevedo, Ricardo Bentes de; Araújo Júnior, Raimundo Fernandes de; Costa, Antonio de Lisboa LopesBackground and Objective Emerging evidence suggests that photodynamic therapy (PDT) can exhibit immunomodulatory activity. The purpose of the present study was to analyse cytokine profiles after application of PDT in gingival tissues of rats with ligature-induced periodontal disease (PD). Study Design/Material and Methods Periodontal disease was induced through the introduction of a cotton thread around the first left mandibular molar, while the right side molars did not receive ligatures. After 7 days of PD evolution, ligatures were removed from the left side, and the animals were randomically divided into the following treatment groups: I, rats without treatment; II, rats received chloro-aluminum phthalocyanine (AlClPc); III, rats received low-level laser alone; and IV, rats received AlClPc associated with low-level laser (PDT). The animals were killed 7 days after the treatments, and the mandibles were histologically processed to assess morphological and immunohistochemical profile, while gingival tissues were removed for quantification of tumor necrosis factor (TNF)-α, interleukin (IL-)1β and IL-10 expression (by ELISA). Results Histomorphological analysis of periodontal tissues demonstrated that PDT-treated animals show tissue necrosis, as well as lower TNF- α expression, compared to ligatured animals treated with AlClPc alone. Conclusions It was concluded that PDT using AlClPc entrapped in a lipid nanoemulsion may be useful in therapies, because of immunomodulatory effects that decreased the inflammatory response and cause tissue destruction.