Navegando por Autor "Azevedo, Fabio Medeiros de"
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Artigo Effect of hydration with Ringer's solution submitted to magnetic field in an experimental model of hepatic ischemia-reperfusion injury in rats(JOURNAL OF SURGICAL AND CLINICAL RESEARCH, 2018-11-09) Carriço, Artur da Silva; Azevedo, Ítalo Medeiros de; Freire, Juliana Mendonça; Souza, Jeovana Pinheiro Fernandes; Azevedo, Fabio Medeiros de; Medeiros, Aldo CunhaPurpose:To evaluate the effect of hydration with Ringer's solution submitted to magnetic field in experimental model of hepatic ischemia/reperfusion in rats. Methods:Wistar rats weighing 389.58±46.88g were randomly allocated into 2 groups: control group (n = 6) -Hepatic ischemia/reperfusion and hydration with Ringer's solution; and test group (n = 6) -Hepatic ischemia/reperfusion and hydration with Ringer submitted to magnetic field of 20 mT during 2 hours. After anesthesia with ketamine 70 mg/kg and xylazine 7mg/kg i.p., the animals were weighed and operated by aseptic technique. The ischemia of the median and left liver lobes was induced for 30 minutes using a vascular clip. Then, i.v. hydration with 3 ml of Ringer in the control group, and 3 ml of magnetizedRinger’s solution in the test group. After 24 hours, blood sample and fragment of the left liver lobe were collected for the determination of AST, ALT, ALP and albumin levels, and histopathological examination. Results:There was no significant differencein comparison of the rats weight loss, biochemical measurements and histopathological scores between the groups (p>0.05). Conclusion:The hydration with Ringer's solution submitted to magnetic field after hepatic ischemia/reperfusion in rats did not alterliver unction tests neither histopathological scores, comparing with the control group.Artigo Effect of Ringer's solution submitted to magnetic field in renal ischemia-reperfusion model in rats(JOURNAL OF SURGICAL AND CLINICAL RESEARCH, 2018-11-09) Carriço, Artur da Silva; Medeiros, Aldo Cunha; Souza, Jeovana Pinheiro Fernandes de; Freire, Juliana Mendonça; Azevedo, Italo Medeiros; Azevedo, Fabio Medeiros dePurpose: To evaluate the effect of hydration with simple Ringer's solution submitted to magnetic field in rats under renal ischemia and reperfusion (I/R) model, using biochemical and histopathological evaluation. Methods: Wistar rats were allocated into 2 equal groups of 6 animals each: Renal ischemia/reperfusion group + hydration with magnetic Ringer (mag Ringer). The left kidney underwent ischemia for 30 minutes. The right kidney was not manipulated. Thereafter, i.v. hydration was performed with 3 ml mag Ringer; Group renal I/R + hidration with simple Ringer. The simple Ringer solution was submitted to a magnetic field of 20mT intensity for 2 hours immediately prior to the surgical procedure. After 24 hours, under anesthesia blood was collected for dosing serum urea and creatinine. Histopathological analysis was performed on the left kidney (HE staining). Results: There was no significant difference between the creatinine (p=0.764) and urea (p = 0.926) values between the control and test groups (non-parametric Mann-Whitney test). Comparing the histopathological scores, there was a significant difference between the groups, with higher levels of lesions in the mag Ringer treated group (p=0.025) - Mann-Whitney test. Conclusion: The use of Ringer's solution submitted to magnetic field for intravenous hydration in a renal ischemia/reperfusion model did not alter the renal function and had a negative influence on the histopathological findings on the kidney, when compared to control.