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Dissertação Detecção de bactérias periodontopatogênicas cultiváveis e não cultiváveis em placas ateromatosas(Universidade Federal do Rio Grande do Norte, 2009-01-12) Aquino, Ana Rafaela Luz de; Lima, Kenio Costa de; ; http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4723244A9; ; http://lattes.cnpq.br/7467353968533692; Gurgel, Bruno César de Vasconcelos; ; http://lattes.cnpq.br/4649601602612601; Seabra, Eduardo Gomes; ; http://lattes.cnpq.br/6218058757798526As infecções periodontais compreendem um grupo de condições inflamatórias, causadas por microrganismos que colonizam a superfície dentária, através da formação do biofilme dentário. Atualmente, infecções crônicas como as periodontais têm sido associadas ao desenvolvimento e progressão da aterosclerose. OBJETIVOS: Detectar bactérias periodontopatogênicas cultiváveis e não cultiváveis em placas ateromatosas; buscar fatores associados à presença destas bactérias nas placas ateromatosas e caracterizar a presença de bactérias cultiváveis e não cultiváveis em placas ateromatosas. METODOLOGIA: Foi realizado um estudo seccional, utilizando uma amostra de 30 pacientes que apresentaram o diagnóstico de aterosclerose nas artérias carótidas, coronarianas ou femorais, e foram tratados cirurgicamente através da realização dos procedimentos de angioplastia com implante de stent, bypasse ou endartarectomia. As placas foram coletadas durante as cirurgias realizadas e analisadas através da técnica molecular de PCR para a presença de DNA das bactérias periodontais cultiváveis Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis e Treponema denticola e o filotipo não-cultivável Synergistes species. Os pacientes foram examinados no leito da enfermaria, após as cirurgias, onde também responderam a um questionário cujo objetivo foi buscar fatores associados à presença de bactérias periodontopatogênicas nas placas ateromatosas. RESULTADOS: Todos os pacientes dentados (66,7%) possuíam doença periodontal, sendo 95% severa e 65% generalizada.Nenhuma bactéria periodontopatogênica pesquisada foi encontrada nas placas ateromatosas. No entanto, quatro amostras (13,3%) foram positivas para a presença de bactérias. Destas, três participantes eram dentados, sendo dois portadores de periodontite crônica severa generalizada e um de periodontite crônica severa localizada. Nenhum deles relatou a realização de procedimentos associados a possíveis episódios de bacteremia, como tratamento endodôntico, exodontia nos últimos seis meses ou algum procedimento cirúrgico odontológico. CONCLUSÃO: As bactérias periodontopatogênicas estudadas não foram encontradas nas placas ateromatosas, não pudendo ser estabelecida a prevalência destes patógenos e nem os fatores associados à presença deles nas placas, mas, a detecção das amostras positivas para bactérias abre caminhos para que outros patógenos periodontais e não periodontais sejam estudados na tentativa de elucidar de maneira definitiva a associação ou não entre a doença periodontal e/ou outras infecções e a aterosclerose, através da presença destas bactérias nos ateromasArtigo Expression of glucose transporters 1 and 3 in metastatic and non-metastatic lower lip squamous cell carcinoma(2014) Demeda, Clarissa Favero; Carvalho, Cyntia Helena Pereira de; Aquino, Ana Rafaela Luz de; Nonaka, Cassiano Francisco Weege; Souza, Lélia Batista de; Pinto, Leão PereiraEste estudo objetivou avaliar a imunoexpressão dos transportadores de glicose 1 (GLUT-1) e 3 (GLUT-3) em carcinomas de células escamosas de lábio inferior (CCELI) metastáticos e não-metastáticos. Vinte CCELIs com metástase nodal regional e 20 CCELI sem metástase foram selecionados. Foram analisados a distribuição da imunomarcação e o percentual de imunorreatividade para GLUT-1 e GLUT-3 no centro tumoral e no front de invasão tumoral. A maioria dos tumores (70%) revelou marcação para GLUT-1 em áreas periféricas dos ninhos, lençóis e ilhas de células neoplásicas, ao passo que GLUT-3 revelou predomínio de marcação em áreas centrais (72.5%). Um alto percentual de células positivas para GLUT-1 foi observado no front de invasão e no centro tumoral das lesões metastáticas e não-metastáticas (p>0,05). O percentual de células positivas para GLUT-1 foi superior ao percentual de células positivas para GLUT-3, tanto no front de invasão (p<0,001) quanto no centro tumoral (p<0,001) dos CCELIs. Não foram observadas diferenças significativas no percentual de células positivas para GLUT-1 e GLUT-3 em relação à mestástase nodal, ao estádio clínico ou ao grau histológico de malignidade (p>0,05). Em conclusão, os resultados do presente estudo sugerem um importante papel para GLUT-1 na absorção de glicose nos CCELIs, embora esta proteína não pareça estar envolvida na progressão destes tumores. Por outro lado, a expressão de GLUT-3 pode representar um mecanismo secundário para a absorção de glicose nos CCELIs.Artigo Immunohistochemical expression of nuclear factor B, matrix metalloproteinase 9, and endoglin (CD105) in odontogenic keratocysts, dentigerous cysts, and radicular cysts(2011) Santos, Pedro Paulo de Andrade; Aquino, Ana Rafaela Luz de; Barreto, Alessandra Oliveira; Freitas, Roseana de Almeida; Galvão, Hebel Cavalcanti; Souza, Lelia Batista deOBJECTIVE: The aim of this study was to compare the immunohistochemical expression of nuclear factor κB (NF-κB), matrix metalloproteinase 9 (MMP-9), and CD105 in odontogenic keratocysts (OKCs), dentigerous cysts (DCs), and radicular cysts (RCs). STUDY DESIGN: Twenty cases of OKCs, 20 DCs, and 20 RCs were analyzed. A labeling index (LI), which expresses the percentage of NF-κB-stained nuclei, was calculated for the analysis of NF-κB expression. Expression of MMP-9 in the epithelium and in the capsule of each lesion was scored as 0 (<10% stained cells), 1 (10%-50% stained cells), or 2 (>50% stained cells). In addition, MMP-9 immunostaining was analyzed in endothelial cells of vessels with a conspicuous lumen. The angiogenic index was determined based on the number of anti-CD105 antibody-stained microvessels. RESULTS: In the epithelial component, the NF-κB LI was higher in OKCs than in DCs and RCs (P < .001). Analysis of MMP-9 expression in the epithelial component showed a predominance of score 2 in OKCs (90%), DCs (70%), and RCs (65%; P = .159). Evaluation of the NF-κB LI according to the expression of MMP-9 in the epithelial lining revealed no significant difference between lesions (P = .282). In the fibrous capsule, the highest percentage of MMP-9-stained cells (score 2) was observed in OKCs (P = .100). Analysis of the expression of MMP-9 in the vessels of odontogenic cysts showed a predominance of score 2 in OKCs (80%) and RCs (50%) and of score 1 in DCs (75%; P = .002). Mean microvessel count was high in RCs (16.9), followed by DCs (12.1) and OKCs (10.0; P = .163). No significant difference in microvessel count according to the expression of MMP-9 was observed between groups (P = .689). CONCLUSIONS: The results suggest that the more aggressive biologic behavior of OKCs is related to the higher expression of MMP-9 and NF-κB in those lesions. The differences in the biologic behavior of the lesions studied do not seem to be associated with the angiogenic index.Artigo Impact on quality of life of removable partial denture wearers after 2 years of use(2011) Barreto, Alessandra Oliveira; Aquino, Luana Maria Martins de; Aquino, Ana Rafaela Luz de; Roncalli, Angelo Giuseppe; Amaral, Bruna Aguiar do; Carreiro, Adriana da Fonte PortoThe use of clinical indicators of satisfaction (OHIP) can be applied to evaluate the impact of denture use on patient quality of life, since dental problems and disorders interfere in the normal life of individuals. Aim: This study aimed at evaluating the satisfaction level of patients rehabilitated with removable partial dentures (RPD) after 2 years of use. Methods: An observational study was carried out on 28 patients with a mean age of 45 years, treated with RPD at the Department of Dentistry of the Federal University of Rio Grande do Norte in 2005. Patients signed informed consent and answered the Oral Health Impact Profile (OHIP) questionnaire on three occasions: prior to rehabilitation and at 3 months and 2 years of denture use. Repeated-measures ANOVA was applied for data analysis. Results: A difference was found between data obtained at the moment of fitting and three months after denture use (p<0.001). However, no variation was observed when comparing data from 3 months and 2 years of use (p>0.05). The variables of gender and age did not interfere in the result (p>0.05). Conclusions: The degree of patient satisfaction after RPD installation was significant at the moment of fitting and 3 months after denture use, but no significant difference was found between 3 months and 2 years of denture use.Artigo Implant-supported removable partial denture(2013) Aquino, Ana Rafaela Luz de; Sousa, Samira Albuquerque de; Carreiro, Adriana da Fonte Porto; Germano, Adriano Rocha; Mendonça, Steyner Lima; Holanda, Danielle Vanessa Miranda deThe absence of lower posterior teeth makes the planning and control of removable partial dentures difficult since support is provided by teeth and mucosa, which present different anatomical characteristics. The planning of removable partial dentures supported on a distal implant renders tooth-implant-supported rather than tooth-mucosa-support dentures an option in distal extension cases. In the present case report, a patient with a Kennedy class II dental arch was rehabilitated using a distal implant-supported removable partial denture. Fitting of the implant resulted in more stable occlusion, improving the functionality of the denture and providing greater comfort to the patient. This approach might be a suitable treatment alternative because of its low cost compared to implant-retained dentures. However, long-term follow-up of a series of cases is necessary for the routine use of this treatment method.Artigo Infected orthokeratinized odontogenic cyst: a rare cause of facial cellulitis(2012) Carvalho, Cyntia Helena Pereira de; Aquino, Ana Rafaela Luz de; Nonaka, Cassiano Francisco Weege; Silva, José Sandro Pereira da; Germano, Adriano da Rocha; Pinto, Leão PereiraOrthokeratinized odontogenic cysts (OOCs) are relatively uncommon developmental cysts lined with orthokeratinized epithelium consisting of a prominent granular layer and a basal layer of low cuboidal flattened cells that show no tendency for nuclear palisading. These cysts have been considered a distinct entity from odontogenic keratocysts since they exhibit a less aggressive behavior and a very low rate of recurrence. Developmental odontogenic cysts can become infected but serious complications, such as potentially life-threatening cellulitis, are rare. This report describes a rare case of facial cellulitis secondary to an infected OOC located in the mandible of a 27-year-old man. The relevant literature about the clinical-pathological features of OOC is reviewed.Artigo Longitudinal Clinical Evaluation of Undercut Areas and Rest Seats of Abutment Teeth in Removable Partial Denture Treatment(2011) Aquino, Ana Rafaela Luz de; Barreto, Alessandra Oliveira; Aquino, Luana Maria Martins de; Ferreira, Maria Angela Fernandes; Carreiro, Adriana da Fonte PortoPURPOSE: Adequate preparation of abutment teeth for removable partial denture (RPD) rest seats allows appropriate masticatory force transmission, retention, and stability of supporting structures. It follows that careful preparation will be important for the longevity of the rehabilitation. The present study aimed to clinically evaluate rest seats and undercut areas of abutment teeth in RPD wearers after 2 years of use. MATERIALS AND METHODS: A total of 193 occlusal, incisal, and cingulum rest seats were evaluated in terms of shape, rest adaptation, wear, caries, fractures, and surface type (enamel, composite resin, or amalgam). Two hundred and fourteen undercut areas were evaluated in terms of surface type (enamel or restoration) and integrity. This study was approved by the Research Ethics Committee of the Federal University of Rio Grande do Norte, resolution 196/1996, protocol number 11/05. RESULTS: Intact preparations accounted for 92.2% of the total. Application of the Pearson test (p= 0.289) found no statistically significant differences among the materials on which the rest seats were prepared. For the undercut areas, 20.7% of those obtained on restorative material were nonintact. In addition, Fisher's exact test showed a statistically significant difference (p= 0.001) in surface type; enamel surfaces were shown to be 14 times more stable than restored surfaces. CONCLUSIONS: The results of this study suggest that rest seats are stable, regardless of the material on which they are prepared. Retentive areas were shown to be more stable when they were located in enamel.Artigo Multiple Second Primary Oral Squamous Cell Carcinomas in a Nonsmoker and Nondrinker Woman: Case Report and Review of the Literature(2015) Lopes, Maria Luiza Diniz de Sousa; Aquino, Ana Rafaela Luz de; Morais, Maria de Lourdes Silva de Arruda; Medeiros, Lélia Bristo de; Silveira, Ericka Janine Dantas daAIM: This manuscript aims to describe an unusual case of multiple second primary squamous cell carcinomas (SCCs) in several sites of the oral mucosa in a nonsmoker and nondrinker woman and to discuss the diagnostic criteria, clinicopathological aspects and outcome of second primary tumor (SPT). BACKGROUND: Patients treated for SCC of the head and neck are at high risk for developing SPT arising from the same dysplastic mucosal feld. Currently, there is no reliable method to predict which of the patients will develop SPT. CASE DESCRIPTION: A 64-year-old nonsmoker and nondrinker woman developed several second primary oral SCCs in 7 years of follow-up, most of them being synchronic, treated by surgery without and with chemotherapy and radiotherapy. CONCLUSION: Patients treated for SCC require a long-term and careful follow-up as the development of SPT contributes with significantly negative impact on the prognosis. CLINICAL SIGNIFICANCE: This report describes the diagnosis and management of a very unusual case of several SPTs affecting different sites of the oral mucosa in the same patient. Moreover, the patient had no apparent risk factors associated with the development of the oral cancer. Therefore, a brief update concerning SPT and its diagnosis and management is also provided.Artigo Prevention of oral lesions in children with acute lymphoblastic leukemia(ELSEVIER, 2006) Pinto, Leao Pereira; Souza, Lelia Batista de; Gordón-Núñez, Manuel Antonio; Soares, Rosilene Calasanz; Costa, Edja Maria Melo de Brito; Aquino, Ana Rafaela Luz de; Fernandes, Maria ZéliaAcute lymphoblastic leukemia (ALL) is the most common form of cancer in children and is responsible for severe stomatologic complications. Treatment consists of four phases of chemotherapy, the main side effect of methotrexate, the drug most used during the intensification phase, is oral mucositis. OBJECTIVE: To evaluate the clinical aspects of the oral mucosa of children with ALL and to determine the effect of 0.12% chlorhexidine gluconate on the prevention of stomatologic complications in these patients. PATIENTS AND METHODS: Thirty-three children treated for ALL ranging in age from 2 to 15 years, without distinction of gender or race, were submitted to visual examination, digital palpation of the oral mucosa and cytologic examination of the buccal mucosa, and divided into two groups: group I consisted of 23 children using an oral solution of 0.12% chlorhexidine gluconate twice a day, and group II consisted of 10 children who did not receive this solution. All children received daily oral hygiene care guided by the dentist throughout treatment. RESULTS: Mucositis was observed in six children of group I and eight of group II, and was characterized by erythema, edema and ulcers. Uniform cytologic findings were obtained for the two groups, with a clear predominance of cells of the intermediate layer in all smears, in addition to a perinuclear halo in 18% of the smears. CONCLUSION: The present results suggest that systematic preventive treatment with 0.12% chlorhexidine gluconate and oral hygiene care reduce the occurrence of oral complications in children with ALL undergoing antineoplastic chemotherapy.Artigo Prótese parcial removível retida por implantes: relato de caso(2013) Aquino, Ana Rafaela Luz de; Sousa, Samira Albuquerque de; Carreiro, Adriana da Fonte Porto; Germano, Adriano Rocha; Mendonça, Steyner Lima; Holanda, Danielle Vanessa Miranda deThe absence of lower posterior teeth makes the planning and control of removable partial dentures difficult since support is provided by teeth and mucosa, which present different anatomical characteristics. The planning of removable partial dentures supported on a distal implant renders tooth-implant-supported rather than tooth-mucosa-support dentures an option in distal extension cases. In the present case report, a patient with a Kennedy class II dental arch was rehabilitated using a distal implant-supported removable partial denture. Fitting of the implant resulted in more stable occlusion, improving the functionality of the denture and providing greater comfort to the patient. This approach might be a suitable treatment alternative because of its low cost compared to implant-retained dentures. However, long-term follow-up of a series of cases is necessary for the routine use of this treatment method.