Navegando por Autor "André, Eunice"
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Dissertação O antagonismo do receptor de serotonina do tipo 7 da substância cinzenta periaquedutal dorsal reduz a ansiedade experimental basal, mas não aquela gerada pela retirada do etanol em ratos(Universidade Federal do Rio Grande do Norte, 2014-09-02) Silveira, Marana Ali; Rachetti, Vanessa de Paula Soares; ; http://lattes.cnpq.br/1524215726056886; ; http://lattes.cnpq.br/8360948057275383; Gavioli, Elaine Cristina; ; http://lattes.cnpq.br/1759328747578795; André, Eunice; ; http://lattes.cnpq.br/8906770743620827Indivíduos dependentes de etanol que diminuem ou interrompem a sua utilização podem apresentar a Síndrome de Abstinência do Álcool, caracterizada por sinais e sintomas desagradáveis que favorecem a recaída, dentre eles, a ansiedade. Por ser uma droga psicotrópica, o etanol é capaz de promover mudanças comportamentais e neurofisiológicas, atuando sobre diversos sistemas de neurotransmissão, dentre outros o sistema serotonérgico, que vem sendo diretamente relacionado aos estados aversivos, como é o caso da ansiedade. O presente estudo teve por objetivo investigar a participação do receptor de serotonina do tipo 7 (5-HT7) da substância cinzenta periaquedutal dorsal (DPAG) na ansiedade experimental basal e naquela gerada pela retirada de etanol. Para isso, ratos Wistar com 75-100 dias foram submetidos a dois experimentos. No primeiro, os animais receberam as doses de 2,5; 5,0 e 10 nmoles do antagonista de receptor 5-HT7 SB269970(SB) ou veículo intra-DPAG e, dez minutos após, foram expostos ao labirinto em cruz elevado (LCE). No dia seguinte, os animais foram submetidos aos mesmos tratamentos e testados no campo aberto (CA). No segundo experimento, os animais receberam concentrações crescentes (2%, 4%, 6%) de etanol como única fonte de dieta líquida ou água (grupo controle), ambos com acesso livre à ração. Setenta e duas e noventa e seis horas após a retirada do etanol, os animais receberam SB (2,5 e 5,0 nmoles) intra-DPAG dez minutos anteriores ao teste no LCE e no CA, respectivamente. No experimento 1, a dose do antagonista de 10 nmoles foi capaz de reverter a ansiedade gerada pela exposição ao LCE. No experimento 2, as doses ineficazes no LCE de SB (2,5 e 5,0 nmoles) não foram capazes de reverter a ansiedade gerada pela retirada de etanol no LCE, embora a dose de 2,5 nmoles de SB tenha revertido o seu efeito hipolocomotor neste teste. Esses resultados sugerem que o receptor 5- HT7 participa modulando a ansiedade experimental basal de ratos, mas não a ansiedade gerada pela retirada do etanol na DPAG.Artigo The elevated T-maze task as an animal model to simultaneously investigate the effects of drugs on long-term memory and anxiety in mice(Elsevier, 2012-04-10) Soares, Bruno Lobão; Asth, Laila; André, Eunice; Soares, Vanessa de Paula; Gavioli, Elaine CristinaThe elevated T-maze (ETM) is an apparatus derived from the elevated plus-maze test, which is used to evaluate anxiety. Because anxiety is a biasing factor in models of memory, this study proposed the ETM as a task for the simultaneous assessment of memory and anxiety in mice. The ETM consists of one enclosed and two open arms. The procedure is based on the avoidance of open spaces learned during training session, in which mice were exposed to the enclosed arm as many times as needed to stay 300 s. In the test session, memory is assessed by re-exposing the mouse to the enclosed arm and the latency to enter an open arm was recorded. The anxiolytic diazepam (DZP; 1 or 2 mg/kg) and the amnestic biperiden (BPR; 0.5, 1 or 3 mg/kg) were injected at three distinct times: pre-training, post-training, and pre-test. Pretraining administration of BPR 1 and DZP 2 increased the number of trials needed to reach the avoidance criterion, suggesting a passive avoidance learning impairment. However, BPR induced hyperlocomotion, which could bias the interpretation of any BPR-induced effects during the training session. Pre-training injection of BPR did not affect the spontaneous increase in the latency to enter an open arm between trials, while DZP reduced latencies in the first three trials suggesting anxiolysis. In the test session, pre-training injection of BPR 1 and DZP 2 reduced latencies to enter an open arm, indicating memory impairment. Post-training and pre-test injection of DZP or BPR did not affect memory. In conclusion, the proposed ETM task is practical for the detection of the anxiolytic and amnesic effects of drugs.Artigo There is more to the picture than meets the rat: A study on rodent geometric shape and proportion preferences(Elsevier, 2015-05-01) Winne, Jéssica; Teixeira, Leslie; Pessoa, Jéssica de Andrade; Gavioli, Elaine Cristina; Rachetti, Vanessa Soares; André, Eunice; Soares, Bruno LobãoIn rodents, the novel object preference test has been used as a behavioral parameter for evaluation of neotic exploratory behavior, and also for memory consolidation tasks. Geometric patterns of this preference are poorly understood, and may vary among species. We evaluated in Wistar rats (Rattus norvergicus) a possible exploration preference considering aluminum tripartite rounded and cylindrical objects of different proportions: 1.2; 1.618; 1.8. At the first day, animals were exposed to 1.2; 1.6 and 1.8 rounded objects. At 24 h after, these animals were exposed to the same objects, together with three new steel cylindrical objects (same proportions). ANOVA and T tests were used to quantify object exploration for each animal (p < 0.05). Data analysis pointed to a longer exploration time of the object 1.2 at the three different protocols indicating a preference pattern on the first day exposition. On the second day the exploration was similar in both familiar and unfamiliar objects, revealing no novel object preference for cylinders. However, we found an object preference related to the 1.2 proportion (balls plus cylinders), in two of three position protocols. In addition, on a single exposition with both cylinders and rounded objects, rats revealed a rounded object preference. The 1.2 preference disclosed by rats also reflected the proportion of their body. From nine main measures of body ratios, seven were close to 1.2 ratio. The correspondence between body ratios and object preference may be explained by habituation learning and by sexual selection, and highlight innate factors regarding aesthetic preferences among species.