CCS - TCC - Farmácia
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TCC Avaliação da toxicidade aguda e eficácia anti-inflamatória do extrato hidroetanólico das raízes da Harpalyce brasiliana Benth no edema de pata induzido por carragenina(Universidade Federal do Rio Grande do Norte, 2021-08-23) Rocha, Enos; Araújo, Renata Mendonça de; Torres-Rêgo, Manoela; 0000-0002-8535-1533; http://lattes.cnpq.br/0735782214033188; 0000-0001-5514-5192; http://lattes.cnpq.br/4163879756935913; 0000-0002-5148-9630; http://lattes.cnpq.br/5043323418030972; Passos, Júlia Gabriela Ramos; 0000-0003-4207-9709; http://lattes.cnpq.br/1969453327313987; Medeiros, Renato Dantas de; 0000-0003-2208-7808; http://lattes.cnpq.br/9604683278634721Harpalyce brasiliana Benth (Leguminoseae), a small tree endemic to the north and northeast of Brazil, popularly known as “snake root”. In folk medicine is used for the treatment of inflammation and snakebites, but there is a shortage of studies that justify its use in popular medicine. So, this study aims to evaluate the cytotoxicity, acute toxicity and anti-inflammatory potential of H. brasiliana root extracts in in vivo model. The cytotoxicity of the extract was analyzed through of hemolytic assay, using doses of 31-2000 µg/mL of extract, and the results demonstrated be safe. The acute toxicity test in vivo was performed through of administration of the of 2000 mg/kg of extract, by gavage route. The animals were observed for 14 days and, at the end of the period, the body and organs were weight and the hematological and biochemical parameters were verified and its was evidenced that not showed alterations significative. For the anti-inflammatory activity in vivo, the carrageenan-induced paw edema model was used. The mice received carrageenan intraplanar and simultaneously was treated, by gavage route, with extract at doses of 100, 200 and 400 mg/kg. In this assay, the extract revealed to decrease of paw edema and myeloperoxidase level. For the first time, our data demonstrate that the hydroethanolic extract from H. brasiliana roots is not cytotoxic, has low toxicity in vivo and has anti-inflammatory effect in preclinical analyses, corroborated its use in folk medicine.TCC Efeito inibitório do extrato hidroetanólico das raízes da Harpalyce brasiliana no envenenamento local induzido pela peçonha da serpente Bothrops leucurus(Universidade Federal do Rio Grande do Norte, 2022-07-01) Cavalcanti, Felipe França; Araujo, Renata Mendonça; Rêgo, Manoela Torres do; 0000-0002-8535-1533; http://lattes.cnpq.br/0735782214033188; 0000-0001-5514-5192; http://lattes.cnpq.br/4163879756935913; 0000-0003-0971-044X; http://lattes.cnpq.br/1305445788644920; Silva, Alessandra Daniele; 0000-0003-1650-3836; http://lattes.cnpq.br/1245062709759151; Silva, Juliana Félix da; 0000-0003-3131-9025; http://lattes.cnpq.br/7927574480239375In northeastern Brazil the Bothrops leucurus is the main causative agent of snakebites. The symptoms of bothropic envenomation are severe pain, edema, hemorrhage, necrosis, amputations and even death. Antivenom serum is not accessible in many regions and present same limitations, requiring alternative therapies. The hydroethanolic extract from the roots of Harpalyce brasiliana has been used for decades as an alternative therapy for snakebites. Therefore, the aim of this study was to evaluate the ability of the hydroethanolic extract of H. brasiliana to inhibit the local effects of B. leucurus snake venom. For this, the assays of inhibition of the enzymatic activity of the venom were carried out in vitro. The evaluation of the inhibition of edematogenic, hemorrhagic and myotoxic activity was performed using experimental models in vivo. The results showed in the enzyme inhibition assays that the extract was not able to decrease phospholipases activity, however it was efficient in hyaluronidase and proteolytic inhibition activities of the venom. In all the concentrations used (100, 200 and 400 mg/kg) the extract was able to significantly reduce the percentage of edema formed, slightly higher than the antivenom serum. Similarly, there was a reduction in myeloperoxidase and pro-inflammatory interleukins levels. Finally, the extract also significantly inhibited the parameters of hemorrhagic and myotoxic activities. These data indicate a potential antiophidic activity of H. brasiliana in poisoning by B. leucurus, especially to reduce local poison effects.