CCS - DNUT - Artigos publicados em periódicos

URI Permanente para esta coleçãohttps://repositorio.ufrn.br/handle/1/56

Navegar

Agora exibindo 1 - 4 de 4

Adipocytes and intestinal epithelium dysfunctions linking obesity to inflammation induced by high glycemic index pellet-diet in wistar rats
(Bioscience Reports, 2018-06) Maciel, Bruna Leal Lima; Luz, Anna Beatriz Santana; Figueredo, Júlia Braga dos Santos; Salviano, Bianca Damásio Pereira Dantas; Aguiar, Ana Júlia Felipe Camelo; Pinheiro, Luiza Gabriella Soares Dantas; Krause, Matheus Felipe Dantas; Camillo, Christina da Silva; Ladd, Fernando Vagner Lobo; Bortolin, Raul Hernandes; Silbiger, Vivian Nogueira; Morais, Ana Heloneida de Araújo
We investigated the inflammatory effect of a pellet-diet with high glycemic index and load (HGLI) on the histological organization of adipocytes, intestinal epithelium, and fat in liver and pancreas in adult male Wistar rats. Two groups (n=10) received for 17 weeks: (1) HGLI diet or (2) Standard diet (LabinaR ). Histological analyses of adipose tissue, jejunum, liver, and pancreas were performed. Stereology analysis, visceral adiposity index, gene expression, and immunohistochemistry of tumor necrosis factor-α (TNF-α) in visceral adipose tissue and plasma TNF-α were also assessed. The HGLI diet-induced hypertrophy of adipocytes with adipocyte volume density equal to 97.0%, cross-sectional area of adipocytes equivalent to 1387 μm2 and a total volume of adipocytes of 6.97 cm3 an elevation of 8%, 25%, and 58%, respectively. Furthermore, the HGLI diet increased liver and pancreatic fat deposition, altered and inflamed the intestinal epithelia, and increased TNF-α gene expression (P=0.014) with a positive immunostaining in visceral adipose tissue and high plasma TNF-α in comparison with standard diet. The results suggest that this diet was able to generate changes commonly caused to solid diets with high fat or fructose-rich beverages. To the best of our knowledge, this is the first report in the literature concerning the properties of low-cost, sucrose-rich pellet-diet presenting high glycemic index and high glycemic load efficient on the development of obesity complications in Wistar rats that were subjected to diet-induced obesity. Therefore, the HGLI pellet-diet may be considered an effective tool to be used by the scientific community in experimental research
Anti-obesity therapeutic targets studied in silico and in vivo: a systematic review
(International Journal of Molecular Sciences, 2024-04) Maia, Juliana Kelly da Silva; Medeiros, Wendjilla Fortunato de; Gomes, Ana Francisca Teixeira; Aguiar, Ana Júlia Felipe Camelo; Queiroz, Jaluza Luana Carvalho de; Bezerra, Ingrid Wilza Leal; Piuvezam, Grasiela; Morais, Ana Heloneida de Araújo
In the age of information technology and the additional computational search tools and software available, this systematic review aimed to identify potential therapeutic targets for obesity, evaluated in silico and subsequently validated in vivo. The systematic review was initially guided by the research question “What therapeutic targets have been used in in silico analysis for the treatment of obesity?” and structured based on the acronym PECo (P, problem; E, exposure; Co, context). The systematic review protocol was formulated and registered in PROSPERO (CRD42022353808) in accordance with the Preferred Reporting Items Checklist for Systematic Review and Meta-Analysis Protocols (PRISMA-P), and the PRISMA was followed for the systematic review. The studies were selected according to the eligibility criteria, aligned with PECo, in the following databases: PubMed, ScienceDirect, Scopus, Web of Science, BVS, and EMBASE. The search strategy yielded 1142 articles, from which, based on the evaluation criteria, 12 were included in the systematic review. Only seven these articles allowed the identification of both in silico and in vivo reassessed therapeutic targets. Among these targets, five were exclusively experimental, one was exclusively theoretical, and one of the targets presented an experimental portion and a portion obtained by modeling. The predominant methodology used was molecular docking and the most studied target was Human Pancreatic Lipase (HPL) (n = 4). The lack of methodological details resulted in more than 50% of the papers being categorized with an “unclear risk of bias” across eight out of the eleven evaluated criteria. From the current systematic review, it seems evident that integrating in silico methodologies into studies of potential drug targets for the exploration of new therapeutic agents provides an important tool, given the ongoing challenges in controlling obesity
In silico structure-based design of peptides or proteins as therapeutic tools for obesity or diabetes mellitus a protocol for systematic review and meta analysis
(Medicine, 2023) Medeiros, Isaiane; Aguiar, Ana Júlia Felipe Camelo; Fortunato, Wendjilla Medeiros; Teixeira, Ana Francisca Gomes; Silva, Emilly Guedes Oliveira e; Bezerra, Ingrid Wilza Leal; Maia, Juliana Kelly da Silva; Piuvezam, Grasiela; Morais, Ana Heloneida de Araújo
Background: In silico studies using dynamic simulation or molecular docking have boosted the screening and identification of molecules and/or targets in studies aimed at treating diseases such as obesity and diabetes mellitus, optimizing the development of new drugs. This study aims to describe a systematic review protocol on peptides and proteins evaluated in silico as potential therapeutic agents for obesity or diabetes mellitus. Methods: This protocol followed the Preferred Reporting Items for Systematic Reviews and Meta-analyses Protocols and was registered in the International Prospective Register of Systematic Reviews database (number: CRD42022355540). The databases to be searched will be PubMed, ScienceDirect, Scopus, Web of Science, virtual health library, and EMBASE. It will be included in silico studies that evaluate the simulation by dynamics or molecular docking of proteins or peptides involved in treating obesity or diabetes mellitus. Two independent reviewers will select studies, extract data, and assess methodological quality using the adapted Strengthening the reporting of empirical simulation studies. A narrative synthesis of the included studies will be performed for the systematic reviews. Results: This protocol contemplates the production of 2 systematic reviews to be developed focusing on obesity or diabetes mellitus. Conclusion: The reviews will enable knowledge of peptides and proteins involved in research treating these diseases and will emphasize the importance of in silico studies in this context and for the development of future studies
Prospecting in silico antibacterial activity of a peptide from trypsin inhibitorisolated from tamarind seed
(Journal of Enzyme Inhibition and Medicinal Chemistry, 2022-08-28) Passos, Thais Souza; Oliveira, Gerciane Silva de; Nascimento, Amanda Maria de Souza; Luz,Anna Beatriz Santana; Aguiar, Ana Júlia Felipe Camelo; Lima, Mayara Santa Rosa; Matias, Lídia Leonize Rodrigues; Amado, Isabel Rodríguez; Damasceno, Karla Suzane Florentino da Silva Chaves; Monteiro, Norberto de Kássio Vieira; Moreira, Susana Margarida Gomes; Pastrana, Lorenzo; Morais, Ana Heloneida de Araújo; https://orcid.org/0000-0003-2054-1544; https://orcid.org/0000-0002-5847-5733; https://orcid.org/0000-0002-6460-911X
Bacterial infections have become a global concern, stimulating the growing demand for natural and bio-logically safe therapeutic agents with antibacterial action. This study was evaluated the genotoxicity ofthe trypsin inhibitor isolated from tamarind seeds (TTI) and the antibacterial effect of TTI theoric model,number 56, and conformation number 287 (TTIp 56/287) and derived peptides in silico. TTI (0.3 and0.6 mg.mL 1 ) did not cause genotoxicity in cells (p > 0.05). In silico, a greater interaction of TTIp 56/287with the Gram-positive membrane (GP) was observed, with an interaction potential energy (IPE) of -1094.97 kcal.mol-1 . In the TTIp 56/287-GP interaction, the Arginine, Threonine (Thr), and Lysine residuespresented lower IPE. In molecular dynamics (MD), Peptidotrychyme59 (TVSQTPIDIPIGLPVR) showed an IPEof -518.08 kcal.mol-1 with the membrane of GP bacteria, and the Thr and Arginine residues showed thegreater IPE. The results highlight new perspectives on TTI and its derived peptides antibacterial activity

SIGAA

Navegar

Navegando CCS - DNUT - Artigos publicados em periódicos por Autor "Aguiar, Ana Júlia Felipe Camelo"